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Identification of Histoplasma capsulatum from culture extracts by real-time PCR.
Martagon-Villamil, Jose; Shrestha, Nabin; Sholtis, Mary; Isada, Carlos M; Hall, Gerri S; Bryne, Terry; Lodge, Barbara A; Reller, L Barth; Procop, Gary W.
J Clin Microbiol ; 41(3): 1295-8, 2003 Mar.
Article in English | MEDLINE | ID: mdl-12624071

ABSTRACT

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.


Subject(s)
Histoplasma/isolation & purification , Polymerase Chain Reaction/methods , DNA, Fungal/analysis , Histoplasma/genetics , Humans , Microbiological Techniques
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