Subject(s)
Animal Care Committees , Animal Welfare/standards , Ethical Review/standards , Models, Animal , Peer Review, Research/standards , Research Design/standards , Animal Experimentation , Animal Rights , Animals , Decision Making , Peer Review, Research/methods , Reproducibility of Results , Time FactorsSubject(s)
Polymerase Chain Reaction/methods , Animal Welfare , Animals , Biotechnology , DNA/genetics , DNA/isolation & purification , Mice , Mice, Transgenic , Pain , SalivaSubject(s)
Analgesia/veterinary , Anesthesia/veterinary , Animal Welfare , Animals, Laboratory , Pain/veterinary , Animals , Research DesignABSTRACT
Male Sprague-Dawley rats were fed semisynthetic diets containing 25% (w/w) experimental lipid. A partially hydrogenated herring oil (PHHO), containing 25% C22:1 as an isomeric mixture dominated by cetoleic (cis-docos-11-enoic), but partially altered to the trans form and containing other positional isomers, was compared with lard:corn oil 3:1(LCO) (w/w). The feeding period extended up to 111 weeks. Ten animals from both groups each were sacrificed at 2, 8, 16, 24, and 32 weeks. The remaining animals from each group were kept on the diets either until spontaneous death occurred or until Week 111. The LCO rats had higher serum cholesterol levels. The PHHO rats had transient myocardial lipidosis and very severe, progressive myocardial inflammation and scarring.
Subject(s)
Cardiomyopathies/etiology , Dietary Fats/toxicity , Fish Oils/toxicity , Animals , Body Weight , Cardiomyopathies/pathology , Cholesterol/blood , Eating , Erucic Acids/toxicity , Lipidoses/etiology , Myocarditis/etiology , Oils/toxicity , Rats , Rats, Inbred Strains , Time FactorsSubject(s)
Animals, Laboratory , Research , Animals , History, 19th Century , History, 20th Century , United States , Vivisection/historyABSTRACT
High levels of the heme precursor, protoporphyrin, were found in a sample of 36 Aotus trivirgatus. The owl monkeys were of five different karyotypes and from three separate colonies. In many animals, a significant proportion of the protoporphyrin was not bound to zinc, as is normal, but existed as the free base protoporphyrin IX. The protoporphyrinemia may be related to major pathologies of captive Aotus.
