ABSTRACT
Two novel HLA-DRB1 alleles were detected using next generation sequencing.
Subject(s)
Alleles , HLA-DRB1 Chains , High-Throughput Nucleotide Sequencing , Histocompatibility Testing , Humans , HLA-DRB1 Chains/genetics , Exons , Sequence Analysis, DNA/methods , Base SequenceABSTRACT
Five novel HLA-C alleles detected by next-generation sequencing: HLA-C*02:02:73, -C*03:04:106, -C*06:382, -C*07:1114Q and -C*12:408.
Subject(s)
Alleles , HLA-C Antigens , High-Throughput Nucleotide Sequencing , Histocompatibility Testing , Humans , High-Throughput Nucleotide Sequencing/methods , HLA-C Antigens/genetics , Histocompatibility Testing/methods , Exons , Sequence Analysis, DNA/methodsABSTRACT
HLA-A*01:454 and HLA-A*31:229, two novel HLA-A alleles detected during routine typing by next-generation sequencing.
Subject(s)
Alleles , Exons , HLA-A Antigens , High-Throughput Nucleotide Sequencing , Histocompatibility Testing , Humans , HLA-A Antigens/genetics , Sequence Analysis, DNA/methods , HLA-A1 Antigen/genetics , Base SequenceABSTRACT
A novel HLA-C*07 allele, now officially designated HLA-C*07:02:150, was identified by next-generation sequencing.
Subject(s)
Genes, MHC Class I , HLA-C Antigens , Humans , HLA-C Antigens/genetics , Alleles , High-Throughput Nucleotide SequencingABSTRACT
Novel HLA-B*55:01:31, HLA-C*07:1113 alleles and confirmatory HLA-C*12:392 allele were detected during the HLA typing process.
Subject(s)
Genes, MHC Class I , HLA-C Antigens , Humans , Alleles , HLA-C Antigens/genetics , HLA-B Antigens/genetics , Histocompatibility TestingABSTRACT
A novel HLA-B*35 allele, officially designated HLA-B*35:594, was identified by next-generation sequencing.
Subject(s)
Alleles , Exons , High-Throughput Nucleotide Sequencing , Histocompatibility Testing , Humans , HLA-B35 Antigen/genetics , Sequence Analysis, DNA , Base Sequence , Codon , HLA-B Antigens/genetics , Tissue DonorsABSTRACT
We identified two novel HLA-DQB1 alleles by NGS, HLA-DQB1*03:519 and HLA-DQB1*06:01:35.
Subject(s)
High-Throughput Nucleotide Sequencing , Humans , Alleles , HLA-DQ beta-Chains/geneticsABSTRACT
Identification of the novel HLA-A*02:1148 and HLA-B*44:386 alleles by next-generation sequencing.
Subject(s)
HLA-A Antigens , High-Throughput Nucleotide Sequencing , Humans , Alleles , HLA-B Antigens/geneticsABSTRACT
HLA-B*35:592 differs from HLA-B*35:03:01:01 by one nucleotide substitution in exon 4.
Subject(s)
Genes, MHC Class I , Nucleotides , Humans , Alleles , Base Sequence , Russia , Sequence Analysis, DNAABSTRACT
We identified two novel HLA alleles by NGS, HLA-B*51:395 and HLA-DQB1*06:478.
Subject(s)
HLA-B Antigens , High-Throughput Nucleotide Sequencing , Humans , Alleles , HLA-DQ beta-Chains/genetics , HLA-B Antigens/geneticsABSTRACT
Detection of radiation-induced changes of the brain white matter is important for brain neoplasms repeated surgery. We investigated the influence of irradiation on the scattering properties of the white matter using optical coherence tomography (OCT). Healthy Wistar rats undergone the irradiation of the brain right hemisphere. At seven time points from the irradiation procedure (2-14 weeks), an ex vivo OCT study was performed with subsequent calculation of attenuation coefficient values in the corpus callosum followed by immunohistochemical analysis. As a result, we discovered acute and early-delayed changes characterized by the edema of different severity, accompanied by a statistically significant decrease in attenuation coefficient values. In particular, these changes were found at 2 weeks after irradiation in the irradiated hemisphere, while at 6- and 12-week time points they affected both irradiated and contralateral hemisphere. Thus, radiation-induced changes occurring in white matter during the first 3 months after irradiation can be detected by OCT.
Subject(s)
Brain Neoplasms , White Matter , Rats , Animals , White Matter/diagnostic imaging , Rats, Wistar , Tomography, Optical Coherence/methods , Brain Neoplasms/diagnostic imaging , Brain/diagnostic imaging , Brain/radiation effectsABSTRACT
Eighteen HLA allele sequences were confirmed and extended: 3 HLA-A, 6 HLA-B, 3 HLA-C, 2 HLA-DRB1, and 4 HLA-DQB1 alleles.
Subject(s)
Bone Marrow , HLA-A Antigens , Humans , Alleles , Gene Frequency , HLA-A Antigens/genetics , Haplotypes , HLA-DQ beta-Chains/genetics , HLA-B Antigens/genetics , HLA-DRB1 Chains/genetics , Registries , RussiaABSTRACT
Characterization of the novel HLA-C*07:1093 allele in a Russian female bone marrow donor.
Subject(s)
HLA-C Antigens , Nucleotides , Humans , Female , HLA-C Antigens/genetics , Alleles , Histocompatibility Testing , Exons/genetics , Tissue DonorsABSTRACT
We identified two novel HLA class II alleles by next-generation sequencing, HLA-DRB1*12:107 and HLA-DQB1*06:476.
Subject(s)
High-Throughput Nucleotide Sequencing , Humans , HLA-DRB1 Chains/genetics , Gene Frequency , Alleles , Haplotypes , HLA-DQ beta-Chains/geneticsABSTRACT
Characterization of the novel HLA-C*07:1083 allele in a Russian bone marrow donor.
Subject(s)
Genes, MHC Class I , HLA-C Antigens , Humans , HLA-C Antigens/genetics , Base Sequence , Alleles , RussiaABSTRACT
The new HLA-DRB1*11:320, HLA-DRB1*15:218, HLA-DQB1*05:324 alleles characterized in bone marrow donors.
Subject(s)
HLA-DRB1 Chains , Humans , HLA-DRB1 Chains/genetics , Gene Frequency , Alleles , Haplotypes , HLA-DQ beta-Chains/geneticsABSTRACT
Vaginal wall prolapse is the most common type of pelvic organ prolapse and is mainly associated with collagen bundle changes in the lamina propria. Neodymium (Nd:YAG) laser treatment was used as an innovative, minimally invasive and non-ablative procedure for the treatment of early-stage vaginal wall prolapse. The purpose of this pilot study was to assess connective tissue changes in the vaginal wall under prolapse without treatment and after Nd:YAG laser treatment using cross-polarization optical coherence tomography (CP OCT) with depth-resolved attenuation mapping. A total of 26 freshly excised samples of vaginal wall from 26 patients with age norm (n = 8), stage I-II prolapses without treatment (n = 8) and stage I-II prolapse 1-2 months after Nd:YAG laser treatment (n = 10) were assessed. As a result, for the first time, depth-resolved attenuation maps of the vaginal wall in the B-scan projection in the co- and cross-polarization channels were constructed. Two parameters within the lamina propria were target calculated: the median value and the percentages of high (≥4 mm-1) and low (<4 mm-1) attenuation coefficient values. A significant (p < 0.0001) decrease in the parameters in the case of vaginal wall prolapse compared to the age norm was identified. After laser treatment, a significant (p < 0.0001) increase in the parameters compared to the normal level was also observed. Notably, in the cross-channel, both parameters showed a greater difference between the groups than in the co-channel. Therefore, using the cross-channel achieved more reliable differentiation between the groups. To conclude, attenuation coefficient maps allow visualization and quantification of changes in the condition of the connective tissue of the vaginal wall. In the future, CP OCT could be used for in vivo detection of early-stage vaginal wall prolapse and for monitoring the effectiveness of treatment.
ABSTRACT
Photodynamic therapy (PDT) is a modern treatment for severe or treatment-resistant vulvar lichen sclerosus (VLS). The chronic and recurrent nature of VLS requires control of recurrences at an early stage. In this paper, a non-invasive multimodal optical coherence tomography (OCT) method was used to control for early histological signs of VLS recurrence after systemic PDT using Photodithazine®. To interpret the OCT data, a histological examination was performed before PDT and 3 months after PDT. Two groups of patients were identified: with early histological signs of VLS recurrence (Group I, n = 5) and without histological signs of VLS recurrence (Group II, n = 6). We use structural OCT, OCT angiography, and OCT lymphangiography throughout 6 months after PDT to visually assess the skin components and to quantitatively assess the dermis by calculating the depth-resolved attenuation coefficient and the density of blood and lymphatic vessels. The OCT data assessment showed a statistically significant difference between the patient groups 3 months after PDT. In Group II, all the studied OCT parameters reached maximum values by the 3rd month after PDT, which indicated recovery of the skin structure. At the same time, in Group I, the values of OCT parameters did not approach the values those in Group II even after 6 months. The obtained results of multimodal OCT can be used for non-invasive control of early histological recurrence of VLS after systemic PDT and for adjusting treatment tactics in advance, without waiting for new clinical manifestations of the disease.
ABSTRACT
HLA-B*13:181 differs from HLA-B*13:02:01:01 by one nucleotide substitution in codon 72 in exon 2.
Subject(s)
Genes, MHC Class I , HLA-B Antigens , Humans , Alleles , Histocompatibility Testing , Codon , HLA-B Antigens/genetics , Sequence Analysis, DNAABSTRACT
Two novel HLA alleles were detected using next generation sequencing technology.
