[Experimental study on the effect of low molecular DNA from salmon milt in pseudotuberculosis infection]. / Izuchenie deistviia nizkomolekuliarnoi DNK iz molok lososevykh ryb pri psevdotuberkuleznoi infektsii v éksperimente.

The effect of low molecular DNA from salmon milt (nDNA) in experimental pseudotuberculosis in mice was studied. When nDNA was admiministered orally, dissemination of the organs by Yersinia pseudotuberculosis lowered and the survival of the animals infected with 100-percent lethal dose of the bacteria increased. nDNA decreased contamination of the epithelial cells by the microbe in vitro and prevented the lethal effect of the Y. pseudotuberculosis toxins on the mice.

[Use of immunomodulators isolated from marine invertebrates for reducing the toxic effects of thermostable toxin and lipopolysaccharides from Yersinia pseudotuberculosis on a macroorganism]. / Ispol'zovanie immunomoduliatorov iz morskikh bespozvonochnykh dlia snizheniia toksicheskogo deistviia termostabil'nogo toksina i lipopolisakkharidov Yersinia pseudotuberculosis na makroorganizm.

The possibility to use immunomodulators isolated from marine invertebrates for the lowering of the toxic effects caused by Yersinia pseudotuberculosis thermoresistant toxin and lipopolysaccharide was investigated. Effects were evaluated by the animals survival rate in per cent and mice average lifetime after toxin lethal dose injection. It was shown that polypeptide gangleen when compared to timalin as well as glycanes mitilane and strombus had dose-dependent protective effect. These substances increased animals survival rate to 15-17 per cent and prolonged life period for about two times when compared to control group. These results demonstrates the possibility to use investigated immunomodulators is clinical practice at the treatment of the patients with pseudotuberculosis.

[Effect of peptide immunomodulator gangleen on the activity of Yersinia pseudotuberculosis thermostable toxin in oxidant and antioxidant systems of human leukocytes]. / Korrektsiia vozdeistviia termostabil'nogo toksina Yersinia pseudotuberculosis na okislitel'nuiu i antiokislitel'nuiu sistemy leikotsitov krovi cheloveka immunomoduliatorom peptidnoi prirody gangliinom.

The ability to correct activity of Y. pseudotuberculosis thermoresistant toxin on antioxidative enzymes and on active oxygen forms in neurophiles and mononuclears was investigated. Toxin at concentration 0.5 and 2.5 mcg/ml did not change O2- production and activity of superoxidedismutase (SOD) and glutathione peroxidase (GP), but significantly enhanced catalase and glutathiont reductase (GR) activity. Gangleen at concentration 0.0002-0.2 mcg/ml, when added to incubation medium with toxin, stimulated production of active oxygen forms and activity of SOD, catalase, GP in both types of leucocytes, but decreased activity of GR in mononuclears. The results of investigation proves the ability to use gangleen in correction of immune system disorders caused by Y. pseudotuberculosis thermoresistant toxin.