Molecular evidence of Opisthorchis viverrini in infected bithyniid snails in the Lao People's Democratic Republic by specific hybridization probe-based real-time fluorescence resonance energy transfer PCR method.

Naturally occurring bithyniid snails, Bithynia siamensis goniomphalos (Prosobranchia: Bithyniidae), and their intermediate hosts were sampled from Khammouane Province, the Lao People's Democratic Republic, and the prevalence of the carcinogenic human liver fluke, Opisthorchis viverrini, was examined. The presence of O. viverrini cercariae in snails was examined by cercarial shedding test and then confirmed by specific hybridization probe-based real-time fluorescence resonance energy transfer (FRET) PCR method. The real-time FRET PCR method is based on a fluorescence melting curve analysis of a hybrid between an amplicon produced from the pOV-A6 specific sequence (Genbank accession no. S80278), a 162-bp repeated sequence specific to O. viverrini, and specific fluorophore-labeled probes. Mean melting temperature of O. viverrini DNA from the cercariae and each of two positive snails by shedding test was 66.3 ± 0.1. The O. viverrini infection rate in snails was 2.47% (2/81) by cercarial shedding test but was 8.52% (4/47) by real-time FRET PCR method. The real-time FRET PCR method is rapid and effective in examining a large number of snail samples simultaneously. Validation using molecular evidence from this procedure provides another tool for surveying the prevalence of O. viverrini-infected snails in Southeast Asian countries.

Snails and trematode infection after Indian Ocean tsunami in Phang-Nga Province, southern Thailand.

The tsunami and non-tsunami affected areas of Takua Pa District, Phang-Nga Province were investigated for fresh- and brackish-water snails that transmit human parasitic diseases during 2006 and 2007. Among 46 snail species found, 17 species of 8 families were freshwater snails, 28 species of another 7 families were brackish-water snails, and 1 species was a land snail. Of these species, 11 freshwater snails, 4 brackish-water snails and 1 land snail were of medical importance. The fresh-water snails were Pomacea canaliculata, Pila angelica, P. gracilis, P. polita, Filopaludina (S.) martensi, F. (F.) s. polygramma, Melanoides tuberculata, Indoplanorbis exuxtus, Radix rubiginosa, Helicorbis umbilicalis, Gyraulus convexiusculus. Four brackish-water snails were Cerithidea cingulata, C. djadjarensis, C. alata, Sermyla riqueti and Achatina fulica was the land snail. I. exutus, M. tuberculata and F. (F.) s. polygramma harbored Xiphidio, Microcercus, Furocercus, Echinostome cercariae, and cercaria without eyespots or tail with hair. Three species of brackish-water snails, Cerithidia cingulata, C. djadjariensis, and C. alata presented with 6 types of trematode cercariae and rediae. Knowledge of medically important snails and their parasitic diseases, and prevention were given to Takua Pa people by poster, pamphlets and broadcasting through community radio.

Intertidal snail-trematode communities on the southern Thailand before and after the South Asia tsunami.

Intertidal snail-trematode communities in southern Thailand were examined before and after the South Asia tsunami. Infection rates and species diversity of cercaria in the host snail Cerithidea in tidal zones did not change significantly from one year before to one month after the tsunami. However, the host snails C. quadrata, C. alata and C. obtusa disappeared from greatly damaged sites. It is important to follow up on the intertidal snail-trematode community recovery process after destruction of the intertidal ecosystem.

Malacological survey in Phang-Nga Province, southern Thailand, pre- and post-Indian Ocean tsunami.

Three malacological surveys were conducted in the Takua Pa District of Phang-Nga Province, southern Thailand, before and after the Indian Ocean Tsunami disaster. Twenty-nine species of fresh- and brackish-water snails were found, in which 10 species of freshwater snails were present, including live Pila polita; 8 species were of medical importance. Two brackish-water snails, Nerita articulata and Littorinopsis scabra, were absent after the tsunami disaster, while brackish-water Cerithidea cingulata and C. djadjariensis harbored 9 types of trematode cercariae.

The health of workers in a metal autoparts factory in eastern Thailand.

One hundred and seventy-two male employees working in the pressing and store sections of a metal autoparts factory in eastern Thailand participated in the study. The aim of this study was to survey the health and well-being condition of Thai workers prior to corporation initiatives in applied ergonomics with the workers of the company. A retrospective study of official accident information, and questionnaires regarding general information, health, muscular discomfort, accidents, posture disorders, and subjective feelings of fatigue or discomfort were filled out for the survey. The results of the study provided 48 categories of important information on the health and wellness of the employees in their workplace. Regression analysis revealed that, based on the working history of the employees, the small and large pressing sections of the workplace had a greater impact on the muscular discomfort of the employees (0.322) (p = 0.001). Based on the health information, the independent factors influencing the employee's muscular discomfort were frequency of muscular discomfort (0.240) (p = 0.004), no disease of muscle and bone (0.165) (p = 0.025), and finally, regularly taking medicine for muscular pain (0.163) (p = 0.024). The factors influencing accidents in the employees were working where they could be cut by sharp material or metal (0.257) (p = 0.008), muscular discomfort (0.169) (p = 0.059), and not using protective equipment (0.146) (p = 0.076). Thus the applied ergonomics intervention program for preventing worker injuries in the sections studied should be implemented, in order to promote the health and well-being of the employees.

An ergonomics intervention program to prevent worker injuries in a metal autoparts factory.

An ergonomics intervention program (EIP) was conducted with male employees working in the pressing and storage sections of a metal autoparts factory in Samut Prakan Province, Thailand. The objectives of this study were to assess the causes of injuries in the pressing and storage sections of that factory, and to improve working conditions by reducing worker injuries from accidents and low back muscular discomfort, using an EIR The study design used a participatory research approach which was quasi-experimental with pretest-posttest evaluations, with a non-equivalent control group. A total of 172 male participants working in Building A were the target group for assessing causes of injury. A retrospective study of official accident information, and questionnaires for general information, health and muscular discomfort, injury frequency rate (IFR), injury severity rate (ISR), medical expenses, and EIP design. Two groups of employees volunteered for the study on muscular back discomfort. The first group of 35 persons volunteered to participate in the EIP (EIP group), and the second 17 persons from Building B did not (non-EIP group). The EIP was composed of 4 major categories: (1) engineering improvement, (2) change in personal protective equipment, (3) environmental improvement, (4) administrative intervention, training, and health education. Low back muscular discomfort was measured through questionnaires on subjective feelings of muscular discomfort, and by surface electromyography (sEMG). Muscle activities were measured by sEMG of the left and right erector spinae and multifidus muscles, and evaluated by multivariate test for dependent samples (paired observation), and multivariate test for two independent samples. After EIP, IFR decreased 65.46%, ISR decreased 41.02%, and medical expenses decreased 42.79%. The low back muscular loads of the EIP group were significantly reduced, with a 95% confidence level (p < 0.05) while those of the non-EIP group were not. Subjective feelings of muscular discomfort, determined by Wilcoxon Signed Ranks test, showed that after applying the EIP to the EIP group, the mean scores for general bodily discomfort and low back muscular discomfort in the EIP group had significantly reduced, while those of the non-EIP group increased, (p < 0.05).

Brackish-water mollusks of Surat Thani Province, southern Thailand.

Brackish-water mollusks inhabiting the mangrove areas along the Gulf of Thailand of Surat Thani Province were investigated for distribution, abundance and natural infections. Nine families and 32 species of brackish-water snails were recovered from 14 sampling stations. Species belonging to the genus Ceritidea of the family Potamididae were mainly examined and Cerithidea (Cerithideopsilla) Cingulata, C. (C.) djadjariensis, and C. (Cerithidea) charbonnieri were naturally infected with 2 types of trematode cercariae, and one which was undetermined. C. (C.) cingulata had the highest infection rate (38.5%). Viewing two snail communities, the first community on the mainland and the second on Samui Island in Surat Thani Province, 28 brackish-water mollusk species were present on the mainland, 15 species were evident on Samui Island, and 11 snail species were common to both the mainland and Samui Island. Measurement of community similarity based on species presence revealed an index of similarity of 0.51. Concerning land use by the local people in the station areas investigated, brackish-water snails in Surat Thani Province are facing habitat degradation by human use.

Health promotion program for the safe use of pesticides in Thai farmers.

The purpose of this study was to determine the knowledge, attitudes and practices (KAP) concerning the safe use of pesticides of Thai farmers in Don Kha subdistrict, Bang Phae district, Ratchaburi Province. Thirty-three voluntary Thai farmers of thirty-three farming families, recruited by convenience sampling, participated in a training program for six months. Data were collected by questionnaire interviews, and KAP on the safe use of pesticides were compared by paired t-test. Research findings showed that the mean scores of KAP in the posttest were significantly higher than the pretest. The results of this study provided health professionals with information to develop more effective prevention and intervention programs. To prevent illness, the most important role of health officers should to be focus on education and information for individuals, families, and communities.

Reduction of low back muscular discomfort through an applied ergonomics intervention program.

An applied ergonomics intervention program (AEIP) was conducted with male employees who work in the pressing and storage sections of a metal auto parts factory in eastern Thailand. The objective of this study was to reduce worker muscular discomfort at the low back. The study design was a participatory research approach, with quasi-experimental pretest-posttest, and with a non-equivalent control group. Thirty-five persons participated in the AEIP (AEIP group) and 17 persons did not (non-AEIP group). The AEIP was composed of three major categories: (1) top management support; (2) equipment designed for workstations and manual material handling; and (3) administrative intervention, training, and health education. Muscle activity was measured by surface electromyography of the left and right erector spinae, and multifidus muscles; and evaluated by multivariate test for dependent samples (paired observation) and for independent samples. After the AEIP, the low back muscular loads of the AEIP group was significantly reduced, while those of the non-AEIP group were not. Comparison of the means of percentage maximum voluntary contractions (% MVC) of low back muscular activity between the AEIP group and non-AEIP group indicated that the AEIP group had significantly reduced low back muscular load, with a 95% confidence level (p-value < 0.05).