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Chem Commun (Camb) ; 53(88): 11992-11995, 2017 Nov 14.
Article in English | MEDLINE | ID: mdl-28984884

ABSTRACT

The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2',5'-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.


Subject(s)
DNA, Catalytic/chemistry , Fluorescent Dyes/chemistry , Guanosine Triphosphate/chemistry , RNA/analysis , RNA/chemistry , Staining and Labeling/methods , DNA, Catalytic/metabolism , Fluorescent Dyes/chemical synthesis , Guanosine Triphosphate/chemical synthesis , Sulfhydryl Compounds/chemistry
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