ABSTRACT
In spring 2016, Greece reported an outbreak caused by a previously undescribed Salmonella enterica subsp. enterica serotype (antigenic formula 11:z41:e,n,z15) via the Epidemic Intelligence Information System for Food- and Waterborne Diseases and Zoonoses (EPIS-FWD), with epidemiological evidence for sesame products as presumptive vehicle. Subsequently, Germany, Czech Republic, Luxembourg and the United Kingdom (UK) reported infections with this novel serotype via EPIS-FWD. Concerned countries in collaboration with the European Centre for Disease Prevention and Control (ECDC) and European Food Safety Authority (EFSA) adopted a common outbreak case definition. An outbreak case was defined as a laboratory-confirmed notification of the novel Salmonella serotype. Between March 2016 and April 2017, 47 outbreak cases were notified (Greece: nâ¯=â¯22; Germany: nâ¯=â¯13; Czech Republic: nâ¯=â¯5; Luxembourg: nâ¯=â¯4; UK: nâ¯=â¯3). Whole genome sequencing revealed the very close genetic relatedness of isolates from all affected countries. Interviews focusing on sesame product consumption, suspicious food item testing and trace-back analysis following Salmonella spp. detection in food products identified a company in Greece where sesame seeds from different countries were processed. Through European collaboration, it was possible to identify and recall sesame spread as one contaminated food item serving as vehicle of infection and trace it back to its origin.
Subject(s)
Disease Outbreaks/statistics & numerical data , Population Surveillance/methods , Salmonella enterica/isolation & purification , Sesamum/microbiology , Europe/epidemiology , Humans , Salmonella Food Poisoning/epidemiology , Salmonella Infections/epidemiology , Salmonella enterica/classification , Salmonella enterica/genetics , Serogroup , Serotyping , Whole Genome SequencingABSTRACT
We investigated 543 Listeria monocytogenes isolates from food having a temporal and spatial distribution compatible with that of the invasive listeriosis outbreak occurring 2012-2016 in southern Germany. Using forensic microbiology, we identified several products from 1 manufacturer contaminated with the outbreak genotype. Continuous molecular surveillance of food isolates could prevent such outbreaks.
Subject(s)
Contact Tracing/methods , Disease Outbreaks , Foodborne Diseases/epidemiology , Listeria monocytogenes/genetics , Listeriosis/epidemiology , Meat/microbiology , Animals , Bacterial Typing Techniques , Electrophoresis, Gel, Pulsed-Field , Food Microbiology , Germany/epidemiology , Humans , Listeria monocytogenes/classification , Listeria monocytogenes/isolation & purification , Listeriosis/transmission , Meat/poisoning , Multilocus Sequence Typing , SwineABSTRACT
Listeriosis patient isolates in Germany have shown a new identical pulsed-field gel electrophoresis (PFGE) pattern since 2012 (nâ¯=â¯66). Almost all isolates (Listeria monocytogenes serotype 1/2a) belonged to cases living in southern Germany, indicating an outbreak with a so far unknown source. Case numbers in 2015 are high (nâ¯=â¯28). No outbreak cases outside Germany have been reported. Next generation sequencing revealed the unique cluster type CT1248 and confirmed the outbreak. Investigations into the source are ongoing.
