Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
Chem Biodivers ; 20(7): e202300536, 2023 Jul.
Article in English | MEDLINE | ID: mdl-37335297

ABSTRACT

In this study, twelve campesterol derivatives (2-13) were prepared by esterification reaction at the hydroxy group in C-3 and catalytic hydrogenation at the carbon-carbon double bond in C-5(6). All obtained compounds were characterized by IR, 1 H-NMR, 13 C-NMR, and MS spectra. Campesterol (1) and its derivatives (2-13) were evaluated in vitro against Staphylococcus aureus (ATCC 6538), Streptococcus mutans (ATCC 0046), Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 15442), and Klebsiella pneumoniae (ATCC 10031) using the microdilution method. Among tested compounds, 4, 6, 9, 11, 12, and 13 displayed the best antibacterial activity. Moreover, to support the antibacterial activity experiments, the investigation of molecular interactions of more active compounds, and also compound 1 and neomycin, used as starting material and positive control, respectively, at the binding site of the target proteins was performed using molecular docking simulations. Four compounds (7, 9, 10 and 11) are herein described for the first time.


Subject(s)
Anti-Bacterial Agents , Phytosterols , Molecular Docking Simulation , Microbial Sensitivity Tests , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry
2.
Phytochemistry ; 203: 113338, 2022 Nov.
Article in English | MEDLINE | ID: mdl-35948140

ABSTRACT

Withajardins, uncommon modified withanolide-type steroids, have been isolated exclusively from plants of the Solanaceae family so far. Two undescribed withajardins and the known tuboanosigenin were isolated from the hexane/EtOAc 1:1 extract from Athenaea velutina leaves. Their structures were established by an extensive analysis of 1D and 2D-NMR and HRMS data. The absolute configuration was determined by X-ray diffraction (withajardin L and tuboanosigenin) and circular dichroism (CD) analyses (withajardin M). The anti-inflammatory activity of compounds was evaluated through the inhibition of the lipopolysaccharide (LPS)-induced nitric oxide (NO), TNF-α, and IL-6 release in RAW264.7 cells. The cell viability effects to RAW 264.7 cells showed IC50 values of 74.4-354.4 µM. The compounds attenuated LPS-induced release of NO and decreased pro-inflammatory cytokines TNF-α and IL-6 in RAW264.7 cells.


Subject(s)
Anti-Inflammatory Agents , Plant Extracts , Solanaceae , Withanolides , Animals , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/pharmacology , Interleukin-6 , Lipopolysaccharides , Mice , Nitric Oxide , Plant Extracts/chemistry , Plant Extracts/pharmacology , RAW 264.7 Cells , Solanaceae/chemistry , Tumor Necrosis Factor-alpha , Withanolides/chemistry , Withanolides/pharmacology
3.
Colloids Surf B Biointerfaces ; 175: 256-263, 2019 Mar 01.
Article in English | MEDLINE | ID: mdl-30544045

ABSTRACT

The ability of some microorganisms to use clarified cashew apple juice as carbon and energy source for biosurfactant production was assessed under strict controlled conditions. Twelve strains of Bacillus were isolated and evaluated regarding their biosurfactant production capabilities. The biosurfactant obtained with these selected strains showed the capacity of decreasing the surface tension of water from 72.0 to 31.8 mN.m-1 and the interfacial tension of n-hexadecane to 27.2 mN.m-1, with a critical micelle concentration of 12.5 mg.L-1. Not only did the biosurfactant present excellent stability to pH, temperature and salinity, it also showed emulsifying properties in different hydrocarbons. The behavior of the phase diagrams showed the potential of the produced biosurfactant to obtain relatively-stable emulsions for up to 96 h, which allows for its application in several areas. The semi-purified biosurfactant did not show toxicity against Lactuca sativa (lettuce) or Artemia salina (microcrustacean), presenting an LC50 of 612.27 µ mL-1. The surfactant was characterized as being a cyclic lipopeptide with molecular structure similar to that of surfactin. Furthermore, through the employment of the surfactant produced, the remediation effect in oil-contaminated soil could be significantly improved.


Subject(s)
Bacillus subtilis/metabolism , Environmental Pollution/prevention & control , Environmental Restoration and Remediation/methods , Fruit and Vegetable Juices/microbiology , Petroleum Pollution/prevention & control , Surface-Active Agents/pharmacology , Anacardium/chemistry , Animals , Artemia/drug effects , Artemia/growth & development , Emulsions , Hydrogen-Ion Concentration , Lactuca/drug effects , Lactuca/growth & development , Surface Tension/drug effects , Surface-Active Agents/isolation & purification , Surface-Active Agents/metabolism , Temperature
4.
PLoS One ; 13(12): e0208760, 2018.
Article in English | MEDLINE | ID: mdl-30532263

ABSTRACT

The present study evaluated the effect of supplementing in vitro culture medium with J. insularis compared to FSH on isolated secondary follicles and in vitro maturation of oocytes from those follicles. Secondary follicles were isolated from sheep ovaries and individually cultured for 18 days in α-MEM+ (Control), α-MEM+ supplemented with 100 ng/mL recombinant bovine follicle stimulating hormone (FSH) or with 0.3, 1.25, or 2.5 mg/mL of J. insularis extract (JI0.3, JI1.25, and JI2.5, respectively). Culture medium collected every 2 days was used to measure ROS levels. At the end of the culture period, cumulus oocytes complex (COCs) were collected and matured in vitro. Follicular walls were used for mRNA quantitation. JI0.3 led to a higher (P < 0.05) percentages of intact follicles than other groups after 18 days of culture. While follicular diameter remained unchanged from Day 6 onwards with JI0.3 and FSH, percentages of antral cavity formation were higher (P < 0.05) with JI0.3 at Day 6 than in all other treatments. No differences were observed between controls and treatment groups regarding ROS levels and mRNA expression of genes. Viability of resulting oocytes was higher (P < 0.05) in JI0.3 compared to FSH. Interestingly, in control experiment, supplementation of maturation medium with JI0.3 led to higher (P < 0.05) percentages of metaphase II compared to controls. Although more validations will be needed, it seems that this natural extract could be used as a cheap and easily available alternative to commercial FSH.


Subject(s)
Culture Media , Follicle Stimulating Hormone/administration & dosage , In Vitro Oocyte Maturation Techniques , Justicia , Plant Extracts/administration & dosage , Animals , Dose-Response Relationship, Drug , Female , Gene Expression Regulation, Developmental , Justicia/chemistry , Oocytes/drug effects , Oocytes/growth & development , Oocytes/metabolism , Oogenesis/drug effects , Oogenesis/physiology , Ovarian Follicle/drug effects , Ovarian Follicle/growth & development , Ovarian Follicle/metabolism , Phytochemicals/administration & dosage , Phytochemicals/chemistry , Plant Extracts/chemistry , Plant Leaves/chemistry , RNA, Messenger/metabolism , Reactive Oxygen Species/metabolism , Sheep
SELECTION OF CITATIONS
SEARCH DETAIL
...