ABSTRACT
The immunosuppressive activity of peptidoglycan, obtained from S. aureus was studied in mouse experiments on the model of delayed hypersensitivity (DH) to guinea pig antigens. The study revealed that PG suppressed DH, this action being linked with its insoluble fraction. The soluble part of PG did not suppress DH. Experiments with the use of immunosorbent demonstrated the existence of serological similarity between the active factor of the soluble part of PG and its insoluble part.
Subject(s)
Immunosuppressive Agents/pharmacology , Peptidoglycan/pharmacology , Staphylococcus aureus , Animals , Escherichia coli/immunology , Guinea Pigs , Hypersensitivity, Delayed/immunology , Immunity, Cellular/drug effects , Immunosuppressive Agents/immunology , Immunosuppressive Agents/isolation & purification , Mice , Mice, Inbred CBA , Peptidoglycan/immunology , Peptidoglycan/isolation & purification , Salmonella/immunology , Shigella sonnei/immunology , Solubility , Spleen/immunology , Time FactorsABSTRACT
Aquatic-phenol trichloroacetic and salt extracts of Pseudomonas aeruginosa have been studied for their effect on hypersensitivity of the delayed type to guinea pig antigen in mice. It is found that salt extracts of wild strains contain thermostable immunosuppressive factors which are of the lipid nature, have high molecular weight and can be inactivated by trichloroacetic acid and phenol. The salt extracts of museum strains as well as aquatic-phenol and trichloroacetic extracts obtained from wild and museum strains had no immunosuppressive activity.
