[Design of reference panels of serum for testing the quality of HIV antibody tests in practical laboratories]. / Konstruirovanie otsenochnykh panelei syvorotok dlia testirovaniia kachestva provedeniia analizov na antitela k VICh v prakticheskikh laboratoriiakh.

The authors define the scientific basis for development of panels of reference sera intended for effective control of the quality of enzyme immunoassay of HIV antibody screening. Special attention was paid to developing the technology of preparing standards highly stable serum samples with a preset concentration of anti-HIV antibodies. The resultant panel of reference sera was tried in screening for anti-HIV antibodies by enzyme immunoassay at seven diagnostic laboratories. Mathematical analysis of the results permits the detection of the minimal errors in studies with the use of serum panel of practical laboratories.

[The principles of creating standard serum panels with a low concentration of HIV-1 antibodies for the quality control of commercial immunoenzyme test systems]. / Printsipy sozdaniia standartnykh panelei syvorotok s nizkoi kontsentratsiei antitel k VICh-1 dlia kontrolia kachestva kommercheskikh immunofermentnykh test-sistem.

The main approaches are defined to the production of sera with the required concentration of antibodies to HIV by the dilution method and to formation on their basis of serum panels according to theoretical distribution of optic density (OD) values. It was shown to be principally possible to prepare panels of positive sera with low concentration of HIV-specific antibodies in a dry stable form, and their practical importance in the evaluation of the sensitivity of enzyme immunoassays was demonstrated. The evaluation of the quality of commercial test systems is based on the position and shape of the histogram of OD values distribution for panel sera for the controlled test systems.

[Isolation and characteristics of a new strain of diploid cells from human embryonic lung tissue]. / Poluchenie i kharakteristika novogo shtamma diploidnykh kletok iz émbrional'noi tkani legkogo cheloveka.

A new strain of the embryonic human fibroblasts L-68 was obtained and thoroughly characterized. It completely met all the requirements of the International Committee on the Cells Cultures. This strain can be recommended as a substrate for production of viral vaccines, diagnostic preparations and for research purposes.

[Modification of chromosome aberrations caused by kilham virus in rat embryo cells]. / Modifikatsiia khromosomnykh aberratsii pod deistiviem virusa kilkhéma v kletkakh krysinykh émbrionov.

Mutagenic effect of Kilham virus on the frequency of chromosome aberrations in the cells of primary and continous rat embryo cultures and the modification effect of cadmium salt on the mutagenic potential of this virus was studied. The frequency of chromosome aberrations increased in the primary rat embryo culture after Kilham virus enfection. Rat embryo culture chronically infected with Kilham virus did not differ from control continuous cells in the frequency level of chromosome aberrations. Isertion of cadmium in the process of cultivation increased the mutagenic effect of kilham virus in the primary rat embryo culture.