ABSTRACT
OBJECTIVE: The aim: improving the effectiveness of treatment of dental caries in children using the method of photodynamic therapy. PATIENTS AND METHODS: Materials and methods: The treatment of permanent tooth decay was performed in 35 children of the Upper Tysa region aged 12-15 years. Using the polymerase chain reaction method, the clinical efficacy of photodynamic therapy in the treatment of dentin caries was determined. RESULTS: Results: During the study, by PCR analysis, dentin caries most commonly revealed genetic markers of DNA of the five most virulent anaerobic bacteria: Prevotella intermedia, Fusobacterium spp., Enterococcus Faecalis, Veilonella spp., Candida albicans. After treatment of the carious cavity by the method of photodynamic therapy with exposure of 30 s - fusobacterium spp. were not detected, the detection rate of prevotella intermedia decreased 3-fold, enterococcus faecalis 3.5-fold, veilonella spp.- 5-fold, and candida albicans- 8-fold; after treatment with carious cavities with 60 s exposure - anaerobic microorganisms were not detected in the investigated samples. The effect of photodynamic therapy with laser exposure of 60 seconds on caries-causing streptococci resulted in their total death, and with exposure of 30 seconds - the frequency of isolated strains decreased several folds. CONCLUSION: Conclusions: The use of photodynamic therapy in the treatment of dentine caries is a highly effective and pathogenetically sound method of treatment that provides a significant reduction in the optional and obligate types of cariesogenic microorganisms.
