[Detection of hepatitis B virus DNA using local nucleic acid amplification (polymerase chain reaction) in fixed tissues in chronic viral liver diseases]. / Vyiavlenie DNK virusa gepatita v metodom lokal'noi amplifikatsii nukleinovykh kislot (polimeraznaia tsepnaia reaktsiia) v fiksirovannykh tkaniiakh pri khronicheskikh virusnykh zabolevaniiakh pecheni.

PCR is a highly sensitive, convenient and rapid method for detection of viral DNA in fixed tissue samples which allows one to analyse the material from the pathology files, mainly biopsies. PCR in a fixed material requires larger amounts of DNA-polymerase and longer duration of every stage of thermo-cycles compared to PCR in the purified DNA samples.