[Mass spectrometry virulence marker Vibrio cholerae.]

Identification mass-spectrometry of marker proteins of toxicity of V. cholerae for development the express of diagnostics of the causative agent of cholera on the basis of the computer analysis in the MALDI-ToF format of electronic profiles became the purpose of our research. Subjected to the computer analysis mass and spectrometer electronic passports 140 of strains of V. cholerae with obviously known characteristic of strains which were divided into 2 basic groups in the parameter of existence or lack of a gene of cholera toxin (ctx-and ctx+). We for the first time revealed a taxon - the specific marker protein ceous peak having a molecular mass of 3202 Da characteristic of strains of V.cholerae ctx + and unrepresentative for V.cholerae ctx-.

[The mass-spectrometric analysis of MALDI-TOF in identification and typing of strains of comma bacillus].

The data base "Protein profiles of mass-specters of representatives of species of Vibrio cholerae for program MALDI Biotyper" was used to implement typing of strains of comma bacillus isolated at the territory of the Russian Federation in 2010-2012. Also, analysis of degree of similarity and differences among constant ribosomal proteins was implemented. According the results of MALDI-TOF mass-spectrometry strains of V.cholerae were grouped in two distinct clusters. The first cluster included all epidemically dangerous strains isolated from people arrived in Moscow from India 2010-2012. The second cluster included atoxigenic vibrio with no relation to serogroups O1/O139 isolated from residents of Taganrog in 2011. The analysis of main specters of all collection permitted to identify taxon - specific components distinguishing strains of non-O1/non-O139 from strains of V.cholerae El Tor. Hence, the developed data base of proteom portraits of V.cholerae permits identifying, studying and to typing of agents of cholera and other representatives of V.cholerae species detecting their phylogenetic affinity that is ultimately useful for establishing origin of strains isolated from objects of environment and epidemiological decoding of episodes of disease.

[The application of standard approaches to determine epidemic value of cholera vibrio].

The article deals with the results of testing of new diagnostic anti-lipase preparation and bacterial technique of detection of hemolytic strains of cholera vibrio with purpose to implement them in the laboratory practice and standardization of detection of epidemically dangerous strains of cholera vibrio.

[New nutrient medium for the cultivation and isolation of the plague microbe ChDS-37 as an element of the mobilization reserve of specialized antiepidemic teams of the Russian Inspectorate for the Protection of Consumer Rights and Human Welfare].

A new nutrient medium has been designed to culture and isolate the plague microbe ChDS-37 on the basis of the pancreatic digest of baker's yeast. The results of laboratory tests of the designed medium, by using 10 plague microbe strains and those of approval during the tactical and special training of a specialized antiepidemic team (SAET), suggest that the medium has some advantage over reference media and creates prerequisites for being incorporated into the mobilization reserve of a SAET.

Ultrastructural evidence of invasive activity of Vibrio cholerae.

The development of experimental cholera in suckling rabbits is associated with typical cholerogenic syndrome: the presence of Vibrio cholerae in the blood, bile (in 60 and 70% cases, respectively), small and large intestine (in 100% cases). Simultaneously with enterocyte desquamation and increased permeability of the blood-enterocyte barrier, the vibrios are released into villous stroma and then into the microcirculatory bed. the zot toxin is involved in the mechanism of Vibrio cholerae invasion, the corresponding gene is present in the genome of the studied strain.

Ultrastructural changes in small intestinal lipofibroblasts of suckling rabbits with experimental cholera.

Ultrastructural analysis of the jejunum in suckling rabbits showed that lipofibroblasts localized in the submucosa and adjacent to crypts contain lipid inclusions (granules) with typical "melting" surface. Lipofibroblasts contained moderately widened cisternae of the granular endoplasmic reticulum and few mitochondria with dense matrix and poorly developed cristae. Experimental cholera was usually accompanied by a decrease in the number of lipid inclusions, and only in some cases by accumulation of lipid material. Our results suggest that the material accumulated in granules plays a role in the pathogenesis of cholera.

Ultrastructural changes in smooth muscle cells of the small intestine in suckling rabbits with experimental cholera.

Ultrastructural study of the small intestine in suckling rabbits with experimental cholera revealed involvement of the inner and outer smooth muscle layers into the pathological process. Smooth muscle cells were characterized by vacuolar and fatty degeneration and focal colliquative necrosis. Apoptosis played little role in gastrointestinal motility disturbances. The presence of considerable amounts of fluid in intestinal loops reflects peristaltic dysfunction due to generalized damage to smooth muscle cells.

Ultrastructural peculiarities of intranuclear inclusions in some target cells during endotoxin shock.

Intranuclear inclusions detected in hepatic, renal, and cerebral cells during endotoxemia can be subdivided into true and pseudo inclusions. True inclusions have filamentous and crystalline structure and are rarely found in control animals. However, the number of true inclusions increases during pathology and can serve as a marker of molecular pathology in the cells provoked by endotoxin.