ABSTRACT
A convenient synthesis of oximes of steroidal chalcones (4a-4j) was performed and structural assignment of the products was confirmed on the basis of IR, (1)HNMR, (13)C NMR, MS and analytical data. The synthesized compounds were screened for in vitro antioxidant activity by using DPPH method and in vitro antimicrobial activity against different bacterial and fungal strains by agar diffusion method. The activity of the tested compounds against each microbe varied due to structural differences between them. Presence and position of different substituents on the benzene ring of the chalconyl pendent had a marked effect on the activity of the compounds. From the results it can be inferred that the compounds 4a-j showed significant antioxidant activity and antimicrobial activity against all microbial strains used for testing.
Subject(s)
Anti-Bacterial Agents/pharmacology , Antifungal Agents/pharmacology , Chalcones/pharmacology , Free Radical Scavengers/pharmacology , Oximes/pharmacology , Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/chemistry , Antifungal Agents/chemical synthesis , Antifungal Agents/chemistry , Aspergillus niger/drug effects , Bacillus subtilis/drug effects , Biphenyl Compounds/chemistry , Chalcones/chemical synthesis , Chalcones/chemistry , Disk Diffusion Antimicrobial Tests , Free Radical Scavengers/chemical synthesis , Free Radical Scavengers/chemistry , Free Radicals/chemistry , Oximes/chemical synthesis , Oximes/chemistry , Penicillium chrysogenum/drug effects , Picrates/chemistry , Proteus vulgaris/drug effects , Pseudomonas aeruginosa/drug effects , Staphylococcus epidermidis/drug effects , Structure-Activity RelationshipABSTRACT
An efficient and facile synthesis of 17-pyrazolinyl derivatives of pregnenolone and their evaluation as potential anticancer agents against various human cancer cell lines are reported. The scheme involves the transformation of the starting pregnenolone acetate into pregnenolone, conversion of pregnenolone to the corresponding benzylidine derivatives and finally the conversion of this derivative to the stable steroidal 17-pyrazoline. Various compounds 4b, 4c, 4e, 4f, 4h and 4j showed significant cytotoxic activity especially against HT-29, HCT-15, 502713 cell lines.
