Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Methods Mol Biol ; 2261: 213-227, 2021.
Article in English | MEDLINE | ID: mdl-33420992

ABSTRACT

Flow cytometry enables the simultaneous detection of multiple surface and intracellular antigens for proteomic profiling of cells. This allows characterization and identification of specific cell subtypes within a heterogeneous population and is usually called immunophenotyping. Antigen-specific antibodies, conjugated to various fluorophores, are incubated with the sample to identify each marker. Fluorescent light of various wavelengths can be separated, detected, and converted into a digital signal in a flow cytometer. Here we describe an eight-color experiment to identify key peripheral blood cell types; however, this technique can be expanded to detect more than 30 parameters simultaneously.


Subject(s)
Antigens/analysis , Flow Cytometry , Immunophenotyping , Proteins/analysis , Proteomics , Fluorescent Dyes/chemistry , Humans
SELECTION OF CITATIONS
SEARCH DETAIL
...