Application of xanthan gum and konjac gum to improve the texture, rheological properties and microstructure of Oviductus Ranae gel.

The effects of xanthan gum (XG), konjac gum (KG) and mixed gum of XG and KG (MG) on the gel properties of Oviductus Ranae (OR) were studied using texture analysis, dynamic rheometry, scanning electron microscopy and Fourier transform infrared spectroscopy. The addition of both XG and MG can increase the hardness and water-holding capacity and energy storage modulus of the OR gel, but the addition of KG has the opposite effect. When the mass ratio of XG to KG is 1:1, the hardness (730 g), springiness (1.00) and chewiness (218.48 g) of the OR gel are all maximized. The addition of MG significantly more enhances the hydrogen bond and hydrophobic interaction, compared with the addition of only XG or KG, so the OR gel forms a denser and more stable network structure. Our results provide valuable information for further design and preparation of OR gel foods containing XG and KG.

Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation.

The expressed prostatic secretions (EPSs) of men with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), infertile men and normal men were subjected to microbiological study. EPSs were collected from the subjects, which included 26 normal men, 11 infertile patients and 51 CP/CPPS patients. DNA was extracted from each specimen, and the V3 regions of the 16S rRNA genes were amplified using universal bacterial primers. The results showed that the EPS 16S rRNA gene-positive rate in the CP/CPPS and infertile patients was much higher than in the normal men, but without any difference among the three patient groups. The denaturing gradient gel electrophoresis (DGGE) method was used to characterize the EPS bacterial community structure of the prostate fluid from patients with CP/CPPS or infertility issues. Principal component analysis (PCA) and partial least squares (PLS) analyses of PCR-DGGE profiles revealed that the EPS bacterial community structure differed among the three groups. Three bands were identified as the key factors responsible for the discrepancy between CP/CPPS patients and infertile patients (P<0.05). Two bands were identified as priority factors in the discrepancy of category IIIA and category IIIB prostatitis patients (P<0.05). According to this research, the ecological balance of the prostate and low urethra tract, when considered as a microenvironment, might play an important role in the maintenance of a healthy male reproductive tract.

[Genetic diversity of different geographical populations of Oxya chinensis based on AFLP analysis].

In order to study the genetic diversity and genetic structure of populations, 7 populations of Oxya chinensis from 7 provinces (or cities) of China were analyzed using AFLP technique. A total of 336 reproducible bands were amplified with 7 primer combinations from 128 individuals. Two hundred and ninety-two bands (86.90%) were polymorphic. High genetic diversity was found among O. chinensis populations and Wanning population had higher genetic diversity than other populations. Mantel test (r=0.27, P=0.89) suggested that there was no significant association between genetic distance and geographic distance. Remarkable genetic differentiation was found among populations. Unweighted pair group method average (UPGMA) tree showed that the 7 O. chinensis populations were divided into 3 groups: Changping of Beijing, Tai-yuan of Shanxi and Jining of Shandong populations in the north; Hanzhong of Shaanxi, Changsha of Hunan and Laibin of Guangxi populations in the south; and Wanning of Hainan population. Principal component analysis indicated significant genetic differentiation of the north and the south populations and island and continent populations existed in the 7 O. chinensis populations because of geographic isolation.