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1.
PLoS One ; 17(1): e0262055, 2022.
Article in English | MEDLINE | ID: mdl-35081139

ABSTRACT

Many methodologies are used to predict the genetic merit in animals and plants, but some of them require priori assumptions that may increase the complexity of the model. Artificial neural network (ANN) has advantage to not require priori assumptions about the relationships between inputs and the output allowing great flexibility to handle different types of complex non-additive effects, such as dominance and epistasis. Despite this advantage, the biological interpretability of ANNs is still limited. The aim of this research was to estimate the heritability and markers effects for two traits in Coffea canephora using an additive-dominance architecture ANN and to compare it with genomic best linear unbiased prediction (GBLUP). The data used consists of 51 clones of C. canephora varietal Conilon, 32 of varietal group Robusta and 82 intervarietal hybrids. From this, 165 phenotyped individuals were genotyped for 14,387 SNPs. Due to the high computational cost of ANNs, we used Bagging decision tree to reduce the dimensionality of the data, selecting the markers that accumulated 70% of the total importance. An ANN with three hidden layers was run, each varying from 1 to 40 neurons summing 64,000 neural networks. The network architectures with the best predictive ability were selected. The best architectures were composed by 4, 15, and 33 neurons in the first, second and third hidden layers, respectively, for yield, and by 13, 20, and 24 neurons, respectively for rust resistance. The predictive ability was greater when using ANN with three hidden layers than using one hidden layer and GBLUP, with 0.72 and 0.88 for yield and coffee leaf rust resistance, respectively. The concordance rate (CR) of the 10% larger markers effects among the methods varied between 10% and 13.8%, for additive effects and between 5.4% and 11.9% for dominance effects. The narrow-sense ([Formula: see text]) and dominance-only ([Formula: see text]) heritability estimates were 0.25 and 0.06, respectively, for yield, and 0.67 and 0.03, respectively for rust resistance. The ANN was able to estimate the heritabilities from an additive-dominance genomic architectures and the ANN with three hidden layers obtained best predictive ability when compared with those obtained from GBLUP and ANN with one hidden layer.


Subject(s)
Genomics
2.
PLoS One ; 16(12): e0260997, 2021.
Article in English | MEDLINE | ID: mdl-34965248

ABSTRACT

Breeding programs of the species Coffea canephora rely heavily on the significant genetic variability between and within its two varietal groups (conilon and robusta). The use of hybrid families and individuals has been less common. The objectives of this study were to evaluate parents and families from the populations of conilon, robusta, and its hybrids and to define the best breeding and selection strategies for productivity and disease resistance traits. As such, 71 conilon clones, 56 robusta clones, and 20 hybrid families were evaluated over several years for the following traits: vegetative vigor, incidence of rust and cercosporiosis, fruit ripening time, fruit size, plant height, canopy diameter, and yield per plant. Components of variance and genetic parameters were estimated via residual maximum likelihood (REML) and genotypic values were predicted via best linear unbiased prediction (BLUP). Genetic variability among parents (clones) and hybrid families was detected for most of the evaluated traits. The Mulamba-Rank index suggests potential gains up to 17% for the genotypic aggregate of traits in the hybrid population. An intrapopulation recurrent selection within the hybrid population would be the best breeding strategy because the genetic variability, narrow and broad senses heritabilities and selective accuracies for important traits were maximized in the crossed population. Besides, such strategy is simple, low cost and quicker than the concurrent reciprocal recurrent selection in the two parental populations, and this maximizes the genetic gain for unit of time.


Subject(s)
Coffea/genetics , Disease Resistance/genetics , Hybridization, Genetic , Plant Breeding , Plant Diseases/genetics , Quantitative Trait, Heritable , Environment , Genotype , Likelihood Functions
3.
Rev Sci Instrum ; 92(4): 044101, 2021 Apr 01.
Article in English | MEDLINE | ID: mdl-34243480

ABSTRACT

An experimental approach is described in which well-defined perturbations of the gas feed into an Ambient Pressure X-ray Photoelectron Spectroscopy (APXPS) cell are fully synchronized with the time-resolved x-ray photoelectron spectroscopy data acquisition. These experiments unlock new possibilities for investigating the properties of materials and chemical reactions mediated by their surfaces, such as those in heterogeneous catalysis, surface science, and coating/deposition applications. Implementation of this approach, which is termed perturbation-enhanced APXPS, at the SPECIES beamline of MAX IV Laboratory is discussed along with several experimental examples including individual pulses of N2 gas over a Au foil, a multi-pulse titration of oxygen vacancies in a pre-reduced TiO2 single crystal with O2 gas, and a sequence of alternating precursor pulses for atomic layer deposition of TiO2 on a silicon wafer substrate.

4.
J Synchrotron Radiat ; 28(Pt 2): 588-601, 2021 Mar 01.
Article in English | MEDLINE | ID: mdl-33650571

ABSTRACT

The SPECIES beamline has been transferred to the new 1.5 GeV storage ring at the MAX IV Laboratory. Several improvements have been made to the beamline and its endstations during the transfer. Together the Ambient Pressure X-ray Photoelectron Spectroscopy and Resonant Inelastic X-ray Scattering endstations are capable of conducting photoelectron spectroscopy in elevated pressure regimes with enhanced time-resolution and flux and X-ray scattering experiments with improved resolution and flux. Both endstations offer a unique capability for experiments at low photon energies in the vacuum ultraviolet and soft X-ray range. In this paper, the upgrades on the endstations and current performance of the beamline are reported.

5.
PLoS One ; 15(5): e0233290, 2020.
Article in English | MEDLINE | ID: mdl-32442213

ABSTRACT

Path analysis allows understanding the direct and indirect effects among traits. Multicollinearity in correlation matrices may cause a bias in path analysis estimates. This study aimed to: a) understand the correlation among soybean traits and estimate their direct and indirect effects on gain oil content; b) verify the efficiency of ridge path analysis and trait culling to overcome colinearity. Three different matrices with different levels of collinearity were obtained by trait culling. Ridge path analysis was performed on matrices with strong collinearity; otherwise, a traditional path analysis was performed. The same analyses were run on a simulated dataset. Trait culling was applied to matrix R originating the matrices R1 and R2. Path analysis for matrices R1 and R2 presented a high determination coefficient (0.856 and 0.832, respectively) and low effect of the residual variable (0.379 and 0.410 respectively). Ridge path analysis presented low determination coefficient (0.657) and no direct effects greater than the effects of the residual variable (0.585). Trait culling was more effective to overcome collinearity. Mass of grains, number of nodes, and number of pods are promising for indirect selection for oil content.


Subject(s)
Edible Grain/genetics , Glycine max/genetics , Soybean Oil , Phenotype , Plant Breeding , Quantitative Trait Loci
6.
PLoS One ; 14(4): e0215315, 2019.
Article in English | MEDLINE | ID: mdl-30998705

ABSTRACT

At present, single-trait best linear unbiased prediction (BLUP) is the standard method for genetic selection in soybean. However, when genetic selection is performed based on two or more genetically correlated traits and these are analyzed individually, selection bias may arise. Under these conditions, considering the correlation structure between the evaluated traits may provide more-accurate genetic estimates for the evaluated parameters, even under environmental influences. The present study was thus developed to examine the efficiency and applicability of multi-trait multi-environment (MTME) models by the residual maximum likelihood (REML/BLUP) and Bayesian approaches in the genetic selection of segregating soybean progeny. The study involved data pertaining to 203 soybean F2:4 progeny assessed in two environments for the following traits: number of days to maturity (DM), 100-seed weight (SW), and average seed yield per plot (SY). Variance components and genetic and non-genetic parameters were estimated via the REML/BLUP and Bayesian methods. The variance components estimated and the breeding values and genetic gains predicted with selection through the Bayesian procedure were similar to those obtained by REML/BLUP. The frequentist and Bayesian MTME models provided higher estimates of broad-sense heritability per plot (or heritability of total effects of progeny; [Formula: see text]) and mean accuracy of progeny than their respective single-trait versions. Bayesian analysis provided the credibility intervals for the estimates of [Formula: see text]. Therefore, MTME led to greater predicted gains from selection. On this basis, this procedure can be efficiently applied in the genetic selection of segregating soybean progeny.


Subject(s)
Gene-Environment Interaction , Genotype , Glycine max/genetics , Models, Genetic , Multifactorial Inheritance , Selection, Genetic
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