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1.
JBRA Assist Reprod ; 21(4): 370-375, 2017 12 01.
Article in English | MEDLINE | ID: mdl-29120571

ABSTRACT

PGD for HLA typing is a procedure that can be performed when an affected child requires a transplant to treat a non-hereditary disorder related to the hematopoietic and/or immune system. Hematopoietic stem cell transplantation from an HLA-identical donor provides the best treatment option. Three conventional ovarian stimulation procedures for IVF were performed in a couple with a 10-year-old child diagnosed with T-cell acute lymphoblastic leukemia of high risk. Trophectoderm biopsy and aCGH examination were performed on 15 blastocysts, three on the first IVF procedure, four on the second cycle, and eight on the third. Three euploid blastocysts HLA-compatible with the genome of the affected child were identified. One euploid blastocyst HLA-compatible with the affected child was warmed and transferred, resulting in an HLA-matched live birth. In conclusion, combined aCGH for aneuploidy screening and Karyomapping may be performed in a single biopsy procedure.


Subject(s)
Fertilization in Vitro , Histocompatibility Testing , Female , Hematopoietic Stem Cell Transplantation , Humans , Infant, Newborn , Male , Pregnancy , United States
2.
J Assist Reprod Genet ; 34(9): 1199-1205, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28647786

ABSTRACT

PURPOSES: The purpose of this study is to describe a healthy life birth after a mosaic embryo transfer in oocyte in vitro maturation (IVM). METHODS: Patient received minimal stimulation, starting on day 3 after menstrual period. No hCG trigger was administered. Oocyte retrieval was performed and oocytes were matured for 30 h. After denuding, mature oocytes were inseminated by ICSI. Embryos were cultured until blastocyst stage and biopsied. RESULTS: One euploid embryo after array comprehensive genome hybridization (aCGH) was diagnostic. However, the next-generation sequencing (NGS) re-analysis showed that embryo was a mosaic for chromosome 13 and 21. Nevertheless, pregnancy ultrasound scans and non-invasive prenatal test (NIPT-Verifi-Illumina) indicated a normal fetus development. Finally, a healthy baby was born after 38 weeks. Its weight was 4480 g, head circumference 36 cm, and total length of 51 cm. To confirm that the baby was chromosomically normal, an NGS test was performed in buccal cells, a normal profile was obtained. CONCLUSIONS: Our finding confirmed that mosaic embryo transfer would bring a healthy offspring.


Subject(s)
In Vitro Oocyte Maturation Techniques , Live Birth , Mosaicism/embryology , Oocytes/metabolism , Adult , Blastocyst/metabolism , Embryo Transfer/methods , Female , Humans , Oocyte Retrieval/methods , Oocytes/growth & development , Pregnancy , Pregnancy Outcome , Sperm Injections, Intracytoplasmic
3.
An. Fac. Med. (Perú) ; 78(3): 298-302, 2017. ilus, tab
Article in Spanish | LILACS | ID: biblio-989277

ABSTRACT

Introducción. Los estudios genéticos preimplantacionales son cada vez más utilizados con la esperanza de conseguir mejores tasas de implantación y nacido vivo, así como una disminución en la tasa de abortos; por ello resulta necesario analizar estos procedimientos. Objetivo. Evaluar el desarrollo preimplantacional in vitro con ovodonación y estudio genético Diseño. Cohortes retrospectivas. Lugar. Laboratorio Pranor, 20082013. Material. Ciclos de fecundación in vitro con ovodonación (FIV-OD). Intervenciones. Se evaluaron 2 077 ciclos de FIV-OD, los cuales fueron clasificados en tres cohortes: 1) ciclos con biopsia embrionaria en día 3, mediante una incisión en la zona pelúcida (ZP) embrionaria, para exéresis de una blastómera (n=527); 2) ciclos con incisión láser en día 4 del desarrollo embrionario, como parte del procedimiento para la biopsia de trofoblasto (n=131); y, 3) ciclos sin intervención (n=1 419). Principales medidas de resultado. Tasa de fecundación, tasa de blastulación. Resultados. No existió diferencia significativa en la tasa de fecundación de las 3 cohortes (75,0%, 74,6% y 75,9%, respectivamente, p=0,31). La tasa de blastulación en la cohorte 1 fue 42,5%, mientras que en la cohorte 3 fue 47% (RR=1,085; IC=1,051 a 1,120; p<0,0001). Adicionalmente, la cohorte 2 tuvo 51,9%, con una diferencia estadísticamente significativa de prevención del riesgo de no blastular con respecto a la cohorte 3 (RR=0,906; IC=0,851 a -0,965; p=0,0017). Conclusiones. El desarrollo preimplantacional hasta blastocisto puede mejorar cuando se utiliza el láser embrionario en día cuatro. Es necesario realizar más estudios para confirmar nuestros resultados.


Introduction: Preimplantational genetic studies are used to achieve a better implantation rate and live birth, as well as to decrease the abortion rate; these techniques should be evaluated. Objective: To evaluate preimplantational embryo development in in vitro fertilization cycles with oocyte donation and genetic studies.. Design: Retrospective cohort study. Setting: Pranor Laboratory, 2008-2013. Material: In vitro fertilization with oocyte donation (IVF-OD) cycles. Interventions: 2 077 cycles of IVF-OD were evaluated, separated in 3 cohorts: Cohort 1, cycles with embryo biopsy on day 3, by means of an incision in the zona pellucida (ZP), for excision of a blastomer (n = 527); Cohort 2, cycles with a laser incision at day 4 of embryo development, for biopsy of the trophoblast in the blastocyst stage (n = 131) and; cohort 3, FIV-OD cycles without any intervention (n = 1 419). Main outcome measures: Fertilization rate, blastulation rate. Results: There was no difference in the fertilization rate among the three groups studied (75.0%, 74.6% and 75.9% respectively, p = 0.31). Blastulation rate in cohort 1, was 42.5%, whereas, in cohort 3, it was 47% (RR = 1.085, CI = 1.0511.120; p <0.0001). In addition, the rate for cohort 2 was 51.9%, with statistical significance, which prevents from non-blastulation risk compared with cohort 3 (RR = 0.906; IC = 0.851-0.965; p = 0.0017). Conclusions: Preimplantational embryo development would improve blastocyst formation when laser is performed on day 4. Further studies are needed to confirm our results.

4.
An. Fac. Med. (Perú) ; 74(1): 11-14, ene. 2013. ilus
Article in Spanish | LILACS-Express | LILACS, LIPECS | ID: lil-692349

ABSTRACT

Introducción: De las causas más conocidas en cuanto a la falta del éxito en el embarazo con tratamientos de reproducción asistida son aquellas relacionadas a las aneuploidías cromosómicas presentes en los embriones. El diagnóstico genético preimplantacional (PGD) es una técnica empleada en reproducción asistida para detectar estas anomalías, seleccionando aquellos que sean cromosómicamente normales, para luego transferirlos al útero de la paciente. Los embriones con aneuploidías únicas podrían tener la capacidad de sobrevivir y lograr la implantación, y por lo tanto, sin diagnóstico previo, estas podrían pasar desapercibidas. Objetivos: Determinar la incidencia de aneuploidías únicas en embriones de buena calidad embrionaria en el día 3 de desarrollo hasta blastocisto. Diseño: Estadístico y experimental. Instituciones: Reprogenetics Latinoamérica y Centro de Reproducción asistida, de la Clínica Concebir. Material Biológico: Muestras de biopsia embrionaria. Metodología: Análisis comparativo de resultados a partir de la evaluación de cada muestra obtenida por biopsia en el día tercero y día quinto de desarrollo embrionario, realizando el PGD por hibridación in situ (FISH) y genómica comparada (aCGH), respectivamente. Resultados: El 62,9% de embriones que presentaron monosomías únicas al tercer día de desarrollo embrionario resultaron ser de 8 células. Pero cuando se evaluó por aCGH en día cinco, 42,3% resultó anormal, y de estos 37,5% perteneció al estadio de 8 células. El índice de monosomías únicas en blastocisto resultó ser 57,9% de un total de 84,2% de aneuploidías únicas. Conclusiones: Los embriones de 8 células en el tercer día de desarrollo embrionario son los más probables de llegar al estadio de blastocisto, así como presentar aneuploidías únicas.


Background: Known causes of unsuccessful pregnancy in couples undergoing assisted reproduction treatment include embryo aneuploidies. Preimplantation genetic diagnosis (PGD) is a technique used in assisted reproduction in order to detect these abnormalities, select embryos chromosomally normal and subsequently transfer to the patients’ uterus. Embryos with single aneuploidies may have the ability to survive and achieve unnoticed implantation. Objectives: To determine incidence of single aneuploidies in good quality embryos in third day of development to blastocyst. Design: Statistical and experimental study. Setting: Reprogenetics Latin-America and Assisted Reproduction Center - Concebir. Biologic material: Samples of embryo biopsies. Methods: Comparative analysis of results from evaluation of each sample obtained by embryo biopsy on the third and fifth days of embryonic development, performing PGD by respectively in situ hybridization (FISH) and comparative genomics (aCGH). Results: On third day of embryonic development 62.9% of embryos with single monosomy had 8-cell morphology. Though when evaluated by aCGH in the blastocyst stage 42.3% were abnormal and 37.5% of these belonged to the 8-cell stage. Single monosomies index in the blastocyst stage was 57.9% in 84.2% of single aneuploidies. Conclusions: Eight-cell embryos on the third day of embryonic development are most likely to reach blastocyst stage and have single aneuploidies.

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