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1.
PLoS Negl Trop Dis ; 15(2): e0009177, 2021 02.
Article in English | MEDLINE | ID: mdl-33630852

ABSTRACT

BACKGROUND: In this work, a platform was developed and tested to allow to detect a variety of candidate viral, bacterial and parasitic pathogens, for acute fever of unknown origin. The platform is based on a centrifugal microfluidic cartridge, the LabDisk ("FeverDisk" for the specific application), which integrates all necessary reagents for sample-to-answer analysis and is processed by a compact, point-of-care compatible device. METHODOLOGY/PRINCIPAL FINDINGS: A sample volume of 200 µL per FeverDisk was used. In situ extraction with pre-stored reagents was achieved by bind-wash-elute chemistry and magnetic particles. Enzymes for the loop-mediated isothermal amplification (LAMP) were pre-stored in lyopellet form providing stability and independence from the cold chain. The total time to result from sample inlet to read out was 2 h. The proof-of-principle was demonstrated in three small-scale feasibility studies: in Dakar, Senegal and Khartoum, Sudan we tested biobanked samples using 29 and 9 disks, respectively; in Reinfeld, Germany we tested spiked samples and analyzed the limit of detection using three bacteria simultaneously spiked in whole blood using 15 disks. Overall during the three studies, the FeverDisk detected dengue virus (different serotypes), chikungunya virus, Plasmodium falciparum, Salmonella enterica Typhi, Salmonella enterica Paratyphi A and Streptococcus pneumoniae. CONCLUSIONS/SIGNIFICANCE: The FeverDisk proved to be universally applicable as it successfully detected all different types of pathogens as single or co-infections, while it also managed to define the serotype of un-serotyped dengue samples. Thirty-eight FeverDisks at the two African sites provided 59 assay results, out of which 51 (86.4%) were confirmed with reference assay results. The results provide a promising outlook for future implementation of the platform in larger prospective clinical studies for defining its clinical sensitivity and specificity. The technology aims to provide multi-target diagnosis of the origins of fever, which will help fight lethal diseases and the incessant rise of antimicrobial resistance.


Subject(s)
Fever , Molecular Diagnostic Techniques/methods , Nucleic Acid Amplification Techniques/methods , Point-of-Care Systems , Chikungunya virus , DNA, Bacterial , Diagnosis, Differential , Germany , Humans , Prospective Studies , Salmonella/genetics , Salmonella paratyphi A , Senegal , Sudan
2.
Methods Mol Biol ; 2142: 147-164, 2020.
Article in English | MEDLINE | ID: mdl-32367366

ABSTRACT

Two one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of Zika virus (ZIKV) were developed, based on two different primer design approaches: (1) open source, based on a combination of sequence diversity clustering (phylogeny and principal component analysis) and LAVA algorithm, using 45 whole genome ZIKV sequences retrieved from the National Center for Biotechnology Information (NCBI) database; (2) standard software for LAMP primer design (Primer Explorer V4), using 59 sequences of the ZIKV 3' UTR. The assays were firstly evaluated with External Quality Assessment panels from INSTAND e.V. (Germany) and EVD-LabNet (The Netherlands) including 4 and 12 unknown samples, respectively, and secondly, with 9 human, mosquito, and monkey ZIKV isolates from Africa (Senegal, Ivory Coast, and Uganda) and America (Brazil). The limit of detection as determined by probit analysis was 181 molecules for both RT-LAMP assays, and 100% reproducibility in the assays was obtained for 103 molecules (4/8 repetitions were positive for 102 molecules). Both assays were specific, amplifying only ZIKV RNA and not cross-detecting other arboviruses included in this study.


Subject(s)
Algorithms , Molecular Diagnostic Techniques/methods , Nucleic Acid Amplification Techniques/methods , Software , Zika Virus Infection/diagnosis , Zika Virus/genetics , Africa , Animals , Brazil , Cells, Cultured , Culicidae/virology , Germany , Haplorhini , Humans , Laboratory Proficiency Testing , Limit of Detection , Molecular Diagnostic Techniques/standards , Netherlands , Nucleic Acid Amplification Techniques/standards , RNA, Viral/genetics , Reproducibility of Results , Sensitivity and Specificity , Zika Virus/isolation & purification , Zika Virus Infection/veterinary
3.
Methods Mol Biol ; 2142: 165-179, 2020.
Article in English | MEDLINE | ID: mdl-32367367

ABSTRACT

Molecular detection of Zika virus (ZIKV) is a key element of outbreak management. Multiple PCR and isothermal ZIKV assays targeting different ZIKV sequences have been published. In this study, we compared a qRT-PCR, 2 RT-LAMP assays (based on different primer design approaches), and an RT-RPA for the detection of African and Asian/American lineages of ZIKV isolates from human, mosquito, and monkey. Results showed that RT-LAMP detected 100% of samples with a time threshold (Tt) of 18.01 ± 11.71 min while qRT-PCR detected 88.88% of samples with a Tt of 58.30 ± 16.58 min and RT-RPA 50% of samples with a Tt of 3.70 ± 0.44 min.


Subject(s)
Molecular Diagnostic Techniques/methods , Nucleic Acid Amplification Techniques/methods , Real-Time Polymerase Chain Reaction/methods , Zika Virus Infection/diagnosis , Zika Virus/genetics , Animals , Brazil , Cells, Cultured , Cote d'Ivoire , Culicidae , Haplorhini , Humans , Recombinases/metabolism , Reproducibility of Results , Senegal , Uganda , Zika Virus/isolation & purification , Zika Virus Infection/virology
4.
J Fish Dis ; 42(8): 1191-1200, 2019 Aug.
Article in English | MEDLINE | ID: mdl-31184398

ABSTRACT

Francisella noatunensis subsp. orientalis is a pathogen of tilapia and other warm-water fish for which no vaccines are commercially available. In this study, a whole cell formalin-inactivated vaccine was developed for the first time using the highly virulent isolate STIR-GUS-F2f7 and the oil-based adjuvant Montanide™ ISA 763A VG. The efficacy of the vaccine was assessed in red Nile tilapia via intraperitoneal (i.p.) injection using homologous experimental infection and correlates of protection such as seral antibody production and bacterial loads in the spleen. For immunization, fish were i.p. injected with 0.1 ml of the vaccine, the adjuvant alone or PBS. At 840 degree days post-vaccination, all fish were i.p. injected with 4.0 × 103 CFU/fish of pathogenic bacteria. The RPS at the end of the trial was 100% in the vaccinated group with significantly higher survival than in the adjuvant and control groups. The RPS in the adjuvant group was 42%, and no significant difference was seen in survival between this and the PBS group. Moreover, significantly higher antibody titres in the serum and significantly lower bacterial loads in the spleen were detected in the vaccinated fish by ELISA and qPCR, respectively. These findings highlight the potential of autogenous vaccines for controlling francisellosis in tilapia.


Subject(s)
Autovaccines/administration & dosage , Cichlids , Francisella/immunology , Gram-Negative Bacterial Infections/prevention & control , Vaccination/veterinary , Animals , Gram-Negative Bacterial Infections/immunology , Gram-Negative Bacterial Infections/microbiology , Injections, Intraperitoneal/veterinary , Vaccines, Inactivated/administration & dosage
5.
PLoS Negl Trop Dis ; 12(5): e0006381, 2018 05.
Article in English | MEDLINE | ID: mdl-29813062

ABSTRACT

BACKGROUND: 4 one-step, real-time, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of dengue virus (DENV) serotypes by considering 2,056 full genome DENV sequences. DENV1 and DENV2 RT-LAMP assays were validated with 31 blood and 11 serum samples from Tanzania, Senegal, Sudan and Mauritania. DENV3 and DENV4 RT-LAMP assays were validated with 25 serum samples from Cambodia. METHODOLOGY/PRINCIPAL FINDINGS: 4 final reaction primer mixes were obtained by using a combination of Principal Component Analysis of the full DENV genome sequences, and LAMP primer design based on sequence alignments using the LAVA software. These mixes contained 14 (DENV1), 12 (DENV2), 8 (DENV3) and 3 (DENV4) LAMP primer sets. The assays were evaluated with an External Quality Assessment panel from Quality Control for Molecular Diagnostics. The assays were serotype-specific and did not cross-detect with other flaviviruses. The limits of detection, with 95% probability, were 22 (DENV1), 542 (DENV2), 197 (DENV3) and 641 (DENV4) RNA molecules, and 100% reproducibility in the assays was obtained with up to 102 (DENV1) and 103 RNA molecules (DENV2, DENV3 and DENV4). Validation of the DENV2 assay with blood samples from Tanzania resulted in 23 samples detected by RT-LAMP, demonstrating that the assay is 100% specific and 95.8% sensitive (positive predictive value of 100% and a negative predictive value of 85.7%). All serum samples from Senegal, Sudan and Mauritania were detected and 3 untyped as DENV1. The sensitivity of RT-LAMP for DENV4 samples from Cambodia did not quite match qRT-PCR. CONCLUSIONS/SIGNIFICANCE: We have shown a novel approach to design LAMP primers that makes use of fast growing sequence databases. The DENV1 and DENV2 assays were validated with viral RNA extracted clinical samples, showing very good performance parameters.


Subject(s)
Dengue Virus/genetics , Dengue Virus/isolation & purification , Dengue/virology , Nucleic Acid Amplification Techniques/methods , DNA Primers/genetics , Dengue Virus/classification , Dengue Virus/metabolism , Humans , Nucleic Acid Amplification Techniques/instrumentation , Phylogeny , RNA, Viral/genetics , Reverse Transcription , Tanzania
6.
PLoS Negl Trop Dis ; 12(5): e0006448, 2018 05.
Article in English | MEDLINE | ID: mdl-29813065

ABSTRACT

BACKGROUND: A single-tube one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of chikungunya virus (CHIKV) targeting the conserved 6K-E1 target region was developed. The assay was validated with sera collected from a CHIKV outbreak in Senegal in 2015. METHODOLOGY/PRINCIPAL FINDINGS: A novel design approach by combining Principal Component Analysis and phylogenetic analysis of 110 available CHIKV sequences and the LAMP oligonucleotide design software LAVA was used. The assay was evaluated with an External Quality Assessment panel from the European Network for Diagnostics of "Imported" Viral Diseases and was shown to be sensitive and specific and did not cross-detect other arboviruses. The limit of detection as determined by probit analysis, was 163 molecules, and 100% reproducibility in the assays was obtained for 103 molecules (7/8 repetitions were positive for 102 molecules). The assay was validated using 35 RNA samples extracted from sera, and results were compared with those obtained by quantitative RT-PCR carried out at the Institut Pasteur Dakar, demonstrating that the RT-LAMP is 100% sensitive and 80% specific, with a positive predictive value of 97% and negative predictive value of 100%. CONCLUSIONS/SIGNIFICANCE: The RT-LAMP appeared to show superior performance with material stored for months compared to qRT-PCR and can be therefore recommended for use in infrastructures with poor settings.


Subject(s)
Chikungunya Fever/virology , Chikungunya virus/isolation & purification , Genome, Viral , Nucleic Acid Amplification Techniques/methods , Chikungunya Fever/diagnosis , Chikungunya virus/classification , Chikungunya virus/genetics , DNA Primers/genetics , Humans , Phylogeny , Sensitivity and Specificity
7.
Fish Shellfish Immunol ; 78: 270-278, 2018 Jul.
Article in English | MEDLINE | ID: mdl-29702239

ABSTRACT

The immune response of European sea bass to RGNNV and SJNNV infections has been evaluated by quantifying the transcription of some genes involved in the IFN I system, as well as in the inflammatory and adaptive immune mechanisms. The transcription of IFN-I, ISG-12, ISG-15 and MxA genes has been analyzed in brain and head kidney, showing that RGNNV genotype induces a more intense response of the IFN I system than SJNNV in both organs. In addition, the results obtained indicate the importance of the inflammatory response in nodavirus pathogenesis, with the transcription of IL-8 and TNF-α significantly higher in brain than in head kidney, being RGNNV the strongest inductor. An important difference between the immune response induced by both genotypes refers to the IgM titre in sera, which was higher in SJNNV-inoculated fish. The acquired response is also important locally, since TR-γ transcription is higher in brain than in head kidney (especially in the RGNNV-inoculated group). To our knowledge, this is the first study addressing the sea bass anti-SJNNV immune response.


Subject(s)
Bass/immunology , Fish Diseases/immunology , Immunity, Innate/genetics , Nodaviridae/physiology , Nodaviridae/pathogenicity , Transcription, Genetic/immunology , Animals , Antibodies, Viral/metabolism , Brain/immunology , Head Kidney/immunology , RNA Virus Infections/immunology , Virulence
8.
PLoS One ; 13(2): e0192979, 2018.
Article in English | MEDLINE | ID: mdl-29444148

ABSTRACT

Francisella noatunensis subsp. orientalis (Fno) is the causative agent of piscine francisellosis in warm water fish including tilapia. The disease induces chronic granulomatous inflammation with high morbidity and can result in high mortality. Early and accurate detection of Fno is crucial to set appropriate outbreak control measures in tilapia farms. Laboratory detection of Fno mainly depends on bacterial culture and molecular techniques. Recombinase polymerase amplification (RPA) is a novel isothermal technology that has been widely used for the molecular diagnosis of various infectious diseases. In this study, a recombinase polymerase amplification (RPA) assay for rapid detection of Fno was developed and validated. The RPA reaction was performed at a constant temperature of 42°C for 20 min. The RPA assay was performed using a quantitative plasmid standard containing a unique Fno gene sequence. Validation of the assay was performed not only by using DNA from Fno, closely related Francisella species and other common bacterial pathogens in tilapia farms, but also by screening 78 Nile tilapia and 5 water samples. All results were compared with those obtained by previously established real-time qPCR. The developed RPA showed high specificity in detection of Fno with no cross-detection of either the closely related Francisella spp. or the other tested bacteria. The Fno-RPA performance was highly comparable to the published qPCR with detection limits at 15 and 11 DNA molecules detected, respectively. The RPA gave quicker results in approximately 6 min in contrast to the qPCR that needed about 90 min to reach the same detection limit, taking only 2.7-3 min to determine Fno in clinical samples. Moreover, RPA was more tolerant to reaction inhibitors than qPCR when tested with field samples. The fast reaction, simplicity, cost-effectiveness, sensitivity and specificity make the RPA an attractive diagnostic tool that will contribute to controlling the infection through prompt on-site detection of Fno.


Subject(s)
Fish Diseases/microbiology , Francisella/genetics , Francisella/isolation & purification , Gram-Negative Bacterial Infections/veterinary , Nucleic Acid Amplification Techniques/methods , Tilapia/microbiology , Animals , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , DNA-Directed DNA Polymerase , Egypt , Fisheries , Francisella/pathogenicity , Gram-Negative Bacterial Infections/microbiology , Nucleic Acid Amplification Techniques/statistics & numerical data , Real-Time Polymerase Chain Reaction , Recombinases , Species Specificity
9.
Vet Microbiol ; 178(1-2): 19-30, 2015 Jul 09.
Article in English | MEDLINE | ID: mdl-25935121

ABSTRACT

Salmon pancreas disease (SPD) is one of the most commercially significant viral diseases of farmed Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) in Europe. In this study, the potential for dietary mitigation of the disease using different polyunsaturated fatty acid (PUFA) profiles was assessed in rainbow trout. We experimentally infected fish with salmonid alphavirus subtype 1 (SAV-1), the causative agent of SPD. These fish were fed two diets with different n-3/n-6 PUFA ratio (high omega 3, 3.08, and high omega 6, 0.87). We assessed the influence of the diets on the fatty acid composition of the heart at 0 days post infection (d.p.i.) (after 4 weeks of feeding the experimental diets prior to SAV-1 infection), and sampled infected and control fish at 5, 15 and 30d.p.i. Viral E1 and E2 glycoprotein genes were quantified by two absolute real-time PCRs in all the organs sampled, and significantly lower levels of the virus were evident in the organs of fish fed with high omega 6. Characteristic pathological lesions were identified in infected fish as early as 5d.p.i., with no significant differences in the pathology lesion scores between the two dietary regimes. This study shows that decreasing the n-3/n-6 PUFA ratio in experimental diets of rainbow trout changes the fatty acid content of the fish, and is associated with reduced SAV-1 replication in rainbow trout.


Subject(s)
Alphavirus Infections/veterinary , Alphavirus/physiology , Dietary Fats, Unsaturated/pharmacology , Fish Diseases/virology , Oncorhynchus mykiss , Pancreatic Diseases/veterinary , Alphavirus Infections/metabolism , Animals , Dietary Fats, Unsaturated/metabolism , Fatty Acids, Omega-3/metabolism , Fatty Acids, Omega-6/metabolism , Fish Diseases/metabolism , Myocardium/metabolism , Pancreatic Diseases/metabolism , Pancreatic Diseases/virology , Real-Time Polymerase Chain Reaction/veterinary , Virus Replication/drug effects , Virus Replication/physiology
10.
J Vet Sci ; 13(4): 355-62, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23271176

ABSTRACT

The distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens was examined by immunohistochemistry in the nervous and non-nervous organs of juvenile European seabass (Dicentrarchus labrax) during the course of an intramuscular infection. Histological changes resulting from the infection were evaluated from 3 days to 2 months post-infection. The specific antibody response was also studied 2 months post-challenge. Viral proteins were present throughout the experimental period in the retina (inner nuclear layer, ganglion layer, outer limiting membrane, and outer plexiform layer), brain(cerebellum and tectum opticum), and liver (hepatocytes and endothelial cells). These proteins were also observed in the renal tubular cells, white pulp of spleen, and in fibroblasts and cartilage of caudal fin. This is the first report of RGNNV proteins appearing in these organs, where the immunostaining was only detected at certain sampling times after the onset of mortality. Brain and retina of virus-exposed fish showed high levels of vacuolation, while accumulation of fat vacuoles was observed in the liver. RGNNV infection also induced a specific antibody response as measured by an ELISA. In summary, this is the first study demonstrating the presence of viral proteins in cells of caudal fin, kidney and spleen of European seabass.


Subject(s)
Antigens, Viral/metabolism , Bass , Fish Diseases/virology , Nodaviridae , RNA Virus Infections/veterinary , Animal Fins/metabolism , Animal Fins/virology , Animals , Antibodies, Viral/metabolism , Brain/metabolism , Brain/virology , Enzyme-Linked Immunosorbent Assay/veterinary , Immunohistochemistry/veterinary , In Situ Hybridization/veterinary , Kidney/metabolism , Kidney/virology , Liver/metabolism , Liver/virology , Real-Time Polymerase Chain Reaction/veterinary , Retina/metabolism , Retina/virology , Spleen/metabolism , Spleen/virology
11.
Vet Microbiol ; 154(1-2): 86-95, 2011 Dec 29.
Article in English | MEDLINE | ID: mdl-21783331

ABSTRACT

The distribution of viral genome in the tissues of juvenile European seabass (Dicentrarchus labrax) during the course of a Red Spotted Grouper Nervous Necrosis Virus (RGNNV) infection has not yet been described. The present study addresses this and indicates which target organs may be involved in viral replication. This information should enable more accurate detection of virus in asymptomatic carriers, and in turn help to control the spread of the disease. The aim of this study was to examine the pattern of expression of viral genomic segments RNA1 and RNA2, using two absolute real-time PCRs (RT-qPCR), over the course of a RGNNV infection after administering the virus by intramuscular injection. In situ hybridization was also used to locate the RNA2 viral segment in different organs throughout the infection. The experimental challenge provoked an acute form of viral nervous necrosis (VNN), with a resulting cumulative mortality of 37%. The RT-qPCRs designed allowed the detection of both genomic segments in all the organs tested (nervous and non-nervous tissues) at all sampling times examined. The highest viral RNA copy number was found in eyes, although viral replication appeared to begin in the brain. Viral replication was also recorded in pooled internal organs and in caudal fin. However, the increase in the viral RNA copy number in these organs did not result in an increased viral titre, which may indicate that a productive infection does not take place in non-nervous tissues, possibly due to a failure in a viral post-replication step.


Subject(s)
Bass/virology , Fish Diseases/virology , Genome, Viral , Nodaviridae/pathogenicity , RNA Virus Infections/veterinary , Viral Tropism , Animals , Brain/virology , Eye/virology , In Situ Hybridization , Nodaviridae/genetics , RNA Virus Infections/virology , RNA, Viral/genetics , Real-Time Polymerase Chain Reaction , Sensitivity and Specificity , Virus Replication/genetics
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