ABSTRACT
INTRODUCCIÓN: La exposición de líquido cefalorraquídeo (LCR) de pacientes con esclerosis lateral amiotrófica (ELA) induce efectos citotóxicos en cultivos celulares de neuronas motoras in vitro. MATERIAL Y MÉTODOS: Se seleccionó LCR de 32 pacientes con ELA que previamente habían demostrado efectos citotóxicos. Se implantaron con minibombas osmóticas intracerebroventriculares (ICV) en 28 ratas macho adultas y se dividieron en 3 grupos: 9 ratas de LCR de pacientes no-ELA, 15 ratas de ELA-LCR citotóxico y 4 ratas de una solución salina fisiológica. El LCR se administró por vía ICV de forma continua durante periodos de 20 o 43 días. Se realizó la evaluación clínica, electromiográfica y análisis de tejidos después de sacrificio a los 20, 45 y 82 días tras la cirugía. RESULTADOS: Los estudios inmunohistoquímicos muestran daño en los tejidos con características similares a las encontradas en formas esporádicas de ELA, tales como sobre expresión de cistatina C, transferrina y la proteína en el TDP-43 citoplasmática. Los primeros cambios observados parecían jugar un papel protector por la sobreexpresión de periferina, panAKT, fosfoAKT y metalotioneínas; esta expresión habría disminuido al momento de analizar las ratas que se sacrificaron al día 82, en el que hay un aumento de apoptosis. Los primeros cambios celulares identificados fueron la constatación de activación de la microglía seguido por astrogliosis con sobreexpresión de GFAP y proteína S100B. CONCLUSIONES: Nuestros datos parecen indicar que la ELA podría propagarse a través del LCR, y que la administración ICV de ELA-LCR citotóxico produce cambios similares a los encontrados en las formas esporádicas de la enfermedad
INTRODUCTION: Cerebrospinal fluid (CSF) from amyotrophic lateral sclerosis (ALS) patients induces cytotoxic effects in in vitro cultured motor neurons. MATERIAL AND METHODS: We selected CSF with previously reported cytotoxic effects from 32 ALS patients. Twenty-eight adult male rats were intracerebroventricularly implanted with osmotic mini-pumps and divided into 3 groups: 9 rats injected with CSF from non-ALS patients, 15 rats injected with cytotoxic ALS-CSF, and 4 rats injected with a physiological saline solution. CSF was intracerebroventricularly and continuously infused for periods of 20 or 43days after implantation. We conducted clinical assessments and electromyographic examinations, and histological analyses were conducted in rats euthanised 20, 45, and 82days after surgery. RESULTS: Immunohistochemical studies revealed tissue damage with similar characteristics to those found in the sporadic forms of ALS, such as overexpression of cystatin C, transferrin, and TDP-43 protein in the cytoplasm. The earliest changes observed seemed to play a protective role due to the overexpression of peripherin, AKTpan, AKTphospho, and metallothioneins; this expression had diminished by the time we analysed rats euthanised on day 82, when an increase in apoptosis was observed. The first cellular changes identified were activated microglia followed by astrogliosis and overexpression of GFAP and S100B proteins. CONCLUSION: Our data suggest that ALS could spread through CSF and that intracerebroventricular administration of cytotoxic ALS-CSF provokes changes similar to those found in sporadic forms of the disease
Subject(s)
Humans , Animals , Male , Adult , Rats , Amyotrophic Lateral Sclerosis/cerebrospinal fluid , Cerebrum/pathology , Cerebrospinal Fluid/metabolism , Infusions, Intraventricular , Spinal Cord/pathology , Amyotrophic Lateral Sclerosis/pathology , Cerebrospinal Fluid/chemistry , Cytotoxins/pharmacology , Models, Animal , Motor Neurons/cytology , Motor Neurons , Motor Neurons/metabolism , Cells, CulturedABSTRACT
INTRODUCTION: Cerebrospinal fluid (CSF) from amyotrophic lateral sclerosis (ALS) patients induces cytotoxic effects in in vitro cultured motor neurons. MATERIAL AND METHODS: We selected CSF with previously reported cytotoxic effects from 32 ALS patients. Twenty-eight adult male rats were intracerebroventricularly implanted with osmotic mini-pumps and divided into 3 groups: 9 rats injected with CSF from non-ALS patients, 15 rats injected with cytotoxic ALS-CSF, and 4 rats injected with a physiological saline solution. CSF was intracerebroventricularly and continuously infused for periods of 20 or 43days after implantation. We conducted clinical assessments and electromyographic examinations, and histological analyses were conducted in rats euthanised 20, 45, and 82days after surgery. RESULTS: Immunohistochemical studies revealed tissue damage with similar characteristics to those found in the sporadic forms of ALS, such as overexpression of cystatinC, transferrin, and TDP-43 protein in the cytoplasm. The earliest changes observed seemed to play a protective role due to the overexpression of peripherin, AKTpan, AKTphospho, and metallothioneins; this expression had diminished by the time we analysed rats euthanised on day 82, when an increase in apoptosis was observed. The first cellular changes identified were activated microglia followed by astrogliosis and overexpression of GFAP and S100B proteins. CONCLUSION: Our data suggest that ALS could spread through CSF and that intracerebroventricular administration of cytotoxic ALS-CSF provokes changes similar to those found in sporadic forms of the disease.
Subject(s)
Amyotrophic Lateral Sclerosis/cerebrospinal fluid , Brain/pathology , Cerebrospinal Fluid/metabolism , Infusions, Intraventricular , Spinal Cord/pathology , Adult , Amyotrophic Lateral Sclerosis/pathology , Animals , Cells, Cultured , Cerebrospinal Fluid/chemistry , Cytotoxins/pharmacology , Disease Models, Animal , Humans , Male , Motor Neurons/cytology , Motor Neurons/drug effects , Motor Neurons/metabolism , RatsABSTRACT
We reviewed the progression of spinal rotation in 100 consecutive patients with 119 curves. Eighty-four patients had a single curve and composed this study. Thirty-nine cases were due to failure of segmentation, 38 cases were due to failure of formation, and seven cases were unclassifiable. Sixty-five of the patients eventually underwent spinal arthrodesis, and 19 of the patients were treated with nonoperative means, such as bracing or observation. Cases of unilateral bar, hemivertebra, and wedge vertebra showed progressive rotation, regardless of the type of treatment; however, progression of curve magnitude was limited by spinal arthrodesis. Rotation of the spine was not seen in patients with block vertebrae. Rotation of the spine and progression of curve magnitude varied in cases of complex vertebral deformities. Because of the uncertainty of remaining growth potential in congenitally dysplastic vertebrae, future growth in the spine should be considered before the undertaking of operative procedures.
Subject(s)
Scoliosis/congenital , Scoliosis/therapy , Thoracic Vertebrae/growth & development , Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Male , Orthotic Devices , Prognosis , Rotation , Scoliosis/diagnosis , Spinal Fusion/methods , Thoracic Vertebrae/pathology , Thoracic Vertebrae/surgery , Tomography, X-Ray ComputedABSTRACT
Perioperative cefazolin prophylaxis is commonly employed in posterior spinal fusion (PSF) procedures, which are often lengthy and involve significant blood loss. In this study, serum levels of prophylactically administered cefazolin were measured serially during spinal fusion procedures, and the possible relationship of antibiotic concentrations to blood loss and fluid therapy were examined. Serum antibiotic levels, measured at 30-min intervals, declined with a half-life of 90 min, similar to the previously reported experience with normal adult volunteers. There was no relationship between serum half-life and blood loss or fluid replacement. An explanation for these findings is offered, along with recommendations for dosing.
Subject(s)
Blood Loss, Surgical , Cefazolin/blood , Spinal Fusion , Adolescent , Cefazolin/administration & dosage , Cefazolin/pharmacokinetics , Child , Female , Humans , Injections, Intravenous , Intraoperative Care , Intraoperative Period , Male , Premedication , Scoliosis/blood , Scoliosis/surgery , Time FactorsABSTRACT
Chronic recurrent multifocal osteomyelitis is a rare entity that is easily confused with pyogenic osteomyelitis. Three cases that illustrate the common modes of presentation and roentgenographic findings are presented. The current orthopaedic literature is reviewed, and management guidelines are discussed.
