ABSTRACT
Objective: To verify the performance of a new method for obtaining platelet-rich plasma, while avoiding contaminationof the sample during its processing.Study Design: Twenty healthy patients were selected, from whom 21 ml of blood was ex tracted. W ethen proceededto study the platelets and growth factors in basal blood after centrifuging the sample by using a new closedsystem for obtaining platelet-rich plasma (PRP).Results: After centrifuging the blood sample, double the amount of platelets as that found in basal blood wasobtained. Of the four growth factors analyzed, only the factor similar to insulin (IGF) contained the same concentrationafter the centrifuge process. The platelet-derived growth factor (PDGF) and the vascular growth factor(VGF) were multiplied by six with respect to the basal values and disproportionately increased the levels of thetransforming growth factor â (TGF-â).Conclusions: The new closed method for obtaining PRP, after avoiding contamination of the sample following itsuse, offers levels of platelet concentrate and growth factors necessary for regeneration (AU)
Subject(s)
Humans , Platelet-Rich Plasma/physiology , Specimen Handling/methods , Intercellular Signaling Peptides and Proteins/isolation & purification , Centrifugation/methodsABSTRACT
OBJECTIVE: To verify the performance of a new method for obtaining platelet-rich plasma, while avoiding contamination of the sample during its processing. STUDY DESIGN: Twenty healthy patients were selected, from whom 21 ml of blood was extracted. We then proceeded to study the platelets and growth factors in basal blood after centrifuging the sample by using a new closed system for obtaining platelet-rich plasma (PRP). RESULTS: After centrifuging the blood sample, double the amount of platelets as that found in basal blood was obtained. Of the four growth factors analyzed, only the factor similar to insulin (IGF) contained the same concentration after the centrifuge process. The platelet-derived growth factor (PDGF) and the vascular growth factor (VGF) were multiplied by six with respect to the basal values and disproportionately increased the levels of the transforming growth factor ß (TGF-ß). CONCLUSIONS: The new closed method for obtaining PRP, after avoiding contamination of the sample following its use, offers levels of platelet concentrate and growth factors necessary for regeneration.
Subject(s)
Intercellular Signaling Peptides and Proteins/analysis , Platelet-Rich Plasma/chemistry , Centrifugation , Hematology/methods , Humans , Young AdultABSTRACT
La obtención del plasma rico en plaquetas (PRP) está muy extendida en diferentes especialidades médicas y se están utilizando diferentes métodos. En este artículo hacemos un estudio sobre la obtención de PRP de forma ambulatoria con dos protocolos diferentes y tres centrífugas , analizando los resultados en la concentración de las plaquetas en estos protocolos aplicados a 3 diferentes centrífugas (AU)
Abstract: The use and obtain of platelet rich plasma is very extended in many medical especialities in actuallity; and his get is made for several methods. In this article, we perform a study over to get platelet rich plasma to ambulatory way; we put to use in 2 procedures on 3 centrifugal machines; we obtain diferent concentrations of platelet in the 3 centrifugal machines (AU)
Subject(s)
Humans , Platelet-Rich Plasma , Centrifugation , Oral Surgical Procedures/methods , Intercellular Signaling Peptides and Proteins/analysis , Tissue and Organ Procurement/methodsABSTRACT
La radiología digital está avanzando, de forma que hoy en día se puede equiparar en capacidad diagnóstica a la radiología convencional, ya que la resolución y la capacidad de manipulación de las imágenes mejoran la interpretación por parte del profesional, eliminando el procesado de las películas y disminuyendo las dosis sobre los pacientes (AU)
Digital radiology is evolving in such a way that nowadays is comparable in diagnostic capabilities to conventional radiology, This is so because the improved resolution of the digital images and the ability to manipulate the allow the professional a better interpretation of the results. In addition, the dose absorbed by the patient is reduced as capered to the traditional method and the (..) (AU)
