Application of an anti-HQIgY antibody for the measurement of IgY concentrations of hen's and quail's serum and yolk.

The development of a sensitive ELISA for the measurement of quail IgY (QIgY) was the main purpose of our study. The suitable antibody (AB) was prepared in rabbits. Both quail IgY (QIgY) and hen IgY (HIgY) were precipitated by this developed AB. For this reason it was marked as anti-hen-quail-IgY (a-HQIgY). The purified AB was conjugated with horseradish peroxidase (aHQIgY-HRP) and a sensitive direct ELISA was developed, based on this labeled AB. The prepared aHQIgY AB which was used in this developed ELISA method was suitable for the measurement of total and specific IgY concentration in domestic hen (Gallus domesticus) and Japanese quail (Coturnix coturnix japonica) either. As a result of our experiments it is very likely that there are identical sequences of IgYs of both species. This part of IgY has good antigen character at the same time. Probably, this phenomenon has occurrence in other Galliform species, too. Further investigations will be carried out in this field.

Radioimmunoassay for a new phenothiazine derivative and its application.

Fluphenazine-4-chlorophenoxy-isobutyrate ester, a new phenothiazine derivative was synthesized in the Institute for Drug Research Budapest. Radioimmunoassay was developed for the therapeutic monitoring of the drug level after intramuscular depot injection. The fluphenazine hapten was coupled to BSA by mixed-anhydride method. Antisera were produced to this conjugation in New-Zealand white rabbits and were tested for the antibody-titer. The specificity was tested by the cross-reaction with phenothiazine-analogues and other psychotropics. Strong cross-reaction was found with compounds possessing piperazine in side chain (trifluoperazine, perphenazine), but other psychotropic drugs did not react. Tritium-labelled trifluoperazine (spec. activity: 3.5 TBq/mmol) was used as a tracer in the radioimmunoassay. The detection limit was 75 pg with a CV of < 5% in 50 microliters plasma sample (equivalent to 1.5 ng/ml concentration) and a standard curve in the 3 ng/ml-50 ng/ml GYKI-22441 concentration range showed a CV of < 10%. Preliminary pharmacokinetic study was performed in Beagle dogs after intramuscular depot injection with GYKI-22441 in sesame oil in a dose of 0.1 mg/kg. The GYKI-22441 concentration of the plasma samples were measured by the RIA method during a 28-day interval after the treatment and was evaluated by the MultiCalc Immunoassay Data Management program (Pharmacia).

[Thyroxine and triiodothyronine blood plasma concentrations in sows and newborn pigs]. / Thyroxin- und Trijodthyroninkonzentration im Blutplasma von Schweinefeten und neugeborenen Ferkeln.

Plasma thyroxine (T4) and triiodothyronine (T3) concentrations of 11 sows and their 56 fetuses have been measured by radioimmunoassay between 80--112 days of intrauterine development. Similar measurements were carried out in 20 piglets of 2 litters up to 24 hours of age and in sows; the latter within a period of 3 days before and 3 days after parturition. The average T3 concentration in the fetus was 0.30--0.41 ng/ml, that of T4 showed great variations between the litters. After 106 and 112 days of intrauterine development either a high (84 +/- and 159 +/- 24 ng/ml) or a low T4 concentration (18 +/- 10 and 11 +/- 5ng/ml) has been confirmed. At birth sows had a significantly lower plasma hormone concentration than the newborn piglets. After parturition the T4 concentration than the newborn piglets. After parturition the T4 concentration decreased significantly in the first 12 hours of life, while that of T3 increased significantly. In sows, pre- and intrapartal serum T4 and T3 concentrations were significantly lower than post partum.