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The aim of this study was to investigate histone deacetylase 6 (HDAC6) modifies the heat shock protein 90 (HSP90) and heat shock transcription factor 1 (HSF1) affect the levels of pathological markers such as Aß oligomers (Aßo) and Tau phosphorylation (p-Tau) in APP/PS1 double transgenic mice hippocampal tissues or HT22 neurons as well as the changes in cognitive behavioral functions of mice. (1) APP/PS1 transgenic mice (6 months old, 25 ~ 30 g) were randomly assigned to 5 experimental groups, C57BL/6J mice (6 months old, 25 ~ 30 g) were used as 4 control groups, with 8 mice in each group. All mice underwent intracerebroventricular (i.c.v.) cannulation, and the experimental groups were administered with normal saline (APP + NS group), HDAC6 agonist tubastatin A hydrochloride (TSA) (APP + TSA group) or HDAC6 agonist theophylline (Theo) (APP + Theo group), HSP90 inhibitor Ganetespib (Gane) (APP + Gane group), or a combination of pre-injected Gane by TSA (APP + Gane + TSA group); the control group received i.c.v. injections of Gane (Gane group), TSA (TSA group), Theo (Theo group) or NS (NS group), respectively. (2) Mouse hippocampal neurons HT22 were randomly divided into a control group (Control) and an Aß1-42 intervention group (Aß). Within the Aß group, further divisions were made for knockdown HSP90 (Aß + siHSP90 group), overexpression HSP90 (Aß + OE-HSP90 group), knockdown HSF1(Aß + siHSF1 group) and knockdown HSF1 followed by overexpression HSP90 (Aß + siHSF1 + OE-HSP90 group), resulting in a total of 6 groups. Morris water maze test was used to evaluate the cognitive behavior of the mice. Western blot and immunohistochemistry or immunofluorescence were performed to detect the levels of HDAC6, HSP90, HSF1, Aß1-42, Tau protein, and p-Tau in the hippocampal tissue or HT22 cells. qRT-PCR was used to measure the levels of hdac6, hsp90, and hsf1 mRNA in the hippocampus or nerve cells. (1) The levels of HDAC6, Aß1-42 and p-Tau were elevated, while HSP90 and HSF1 were decreased in the hippocampal tissue of APP/PS1 transgenic mice (all P < 0.01). Inhibiting HDAC6 upregulated the expressions of HSP90 and HSF1 in the hippocampal tissue of APP/PS1 mice, while decreasing the levels of Aß1-42 and p-Tau as well as improving the spatial cognitive behavior in mice (P < 0.05 or P < 0.01). The opposite effects were observed upon HDAC6 activation. However, inhibiting HSP90 reduced the expression of HSF1 (P < 0.01) and increased the levels of Aß1-42 and p-Tau (P < 0.05 or P < 0.01) but did not significantly affect the expression of HDAC6 (P > 0.05). No significant changes were observed in the aforementioned indicators in the 4 control groups (P > 0.05). (2) In the Aß1-42 intervention group, HDAC6 and Aß1-42, p-Tau expression levels were elevated, while HSP90 and HSF1 expressions were all decreased, and cell viability was reduced (P < 0.05 or P < 0.01). Overexpression of HSP90 upregulated HSF1 expression, decreased the levels of Aß1-42 and p-Tau, and increased cell viability (P < 0.05 or P < 0.01). Knocking down HSP90 had the opposite effect; and knocking down HSF1 increased the levels of Aß1-42 and p-Tau and decreased cells viability (all P < 0.01), but did not result in significant changes in the expression levels of HSP90 (P > 0.05). Inhibiting HDAC6 can upregulate the expressions of HSP90 and HSF1 but reduce the levels of Aß1-42 and p-Tau in the hippocampus of APP/PS1 mice and improvement of cognitive behavioral function in mice; Overexpression of HSP90 can increase HSF1 but decrease Aß1-42 and p-Tau levels in the hippocampal neurons and increase cell activity. It is suggested that HDAC6 may affect the formation of Aß oligomers and the changes in Tau protein phosphorylation levels in the hippocampus of AD transgenic mouse as well as the alterations in cognitive behavioral functions by regulating the HSP90-HSF1 pathway.
Subject(s)
HSP90 Heat-Shock Proteins , Heat Shock Transcription Factors , Hippocampus , Histone Deacetylase 6 , Mice, Inbred C57BL , Mice, Transgenic , Animals , Histone Deacetylase 6/metabolism , Histone Deacetylase 6/antagonists & inhibitors , HSP90 Heat-Shock Proteins/metabolism , HSP90 Heat-Shock Proteins/antagonists & inhibitors , Hippocampus/metabolism , Hippocampus/drug effects , Hippocampus/pathology , Mice , Heat Shock Transcription Factors/metabolism , Signal Transduction/physiology , Signal Transduction/drug effects , Amyloid beta-Peptides/metabolism , Cognition/physiology , Cognition/drug effects , Alzheimer Disease/pathology , Alzheimer Disease/metabolism , tau Proteins/metabolism , Male , Amyloid beta-Protein Precursor/genetics , Amyloid beta-Protein Precursor/metabolismABSTRACT
In this study, we synthesized two new two-dimensional (2D) covalent organic frameworks (COFs), COF-TA and COF-DP, by combining 4-connected D2h-symmetric and 2-connected non-centrosymmetric C2-symmetric building blocks. Unlike the typical sql topology, these COFs exhibit an unconventional kgm topology characterized by a favorable anti-parallel stacking arrangement, which results in a lower energy configuration. Notably, COF-DP, with its unique D-A-D structural motif and photosensitive properties, demonstrates a narrow band gap and excellent photothermal conversion capabilities, making it a promising material for photothermal imaging applications.
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Objective: Schisandra chinensis (Turcz.) Baill. (S. chinensis) is a Traditional Chinese medicinal herb that can be used both for medicinal purposes and as a food ingredient due to its beneficial properties, and it is enriched with a wide of natural plant nutrients, including flavonoids, phenolic acids, anthocyanins, lignans, triterpenes, organic acids, and sugars. At present, there is lack of comprehensive study or systemic characterization of nutritional and active ingredients of S. chinensis using innovative mass spectrometry techniques. Methods: The comprehensive review was conducted by searching the PubMed databases for relevant literature of various mass spectrometry techniques employed in the analysis of nutritional components in S. chinensis, as well as their main nutritional effects. The literature search covered the past 5 years until March 15, 2023. Results: The potential nutritional effects of S. chinensis are discussed, including its ability to enhance immunity, function as an antioxidant, anti-allergen, antidepressant, and anti-anxiety agent, as well as its ability to act as a sedative-hypnotic and improve memory, cognitive function, and metabolic imbalances. Meanwhile, the use of advanced mass spectrometry detection technologies have the potential to enable the discovery of new nutritional components of S. chinensis, and to verify the effects of different extraction methods on these components. The contents of anthocyanins, lignans, organic acids, and polysaccharides, the main nutritional components in S. chinensis, are also closely associated to its quality. Conclusion: This review will provide guidelines for an in-depth study on the nutritional value of S. chinensis and for the development of healthy food products with effective components.
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ABSTRACT Introduction Tai Ji is a martial art rich in traditional Chinese ethnic characteristics. Its flexible, free and open movements make its practice popular and esteemed by many female college students. However, there is a demand for experimental research on the impacts of the art on the physical and mental health of its college practitioners. Objective Verify the effects of Tai Ji practice on the physical health of university students. Methods This study used the methods of literature, experimental and mathematical statistics, through the influence of Tai Ji style number 24 on the physical health of female university students in different groups. Results The research shows that under the intervention of this style, with the same content, intensity, and time of exercise, the physical health of female college students in the low-fitness group was significantly improved. Conclusion Female college students in the medium and high fitness group improved their physical health indicators due to their physical preconditioning. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução O Tai Chi Chuan é uma arte marcial rica em características étnicas tradicionais chinesas. Seus movimentos flexíveis, livres e abertos fazem com que a sua prática seja popular e estimada por uma ampla gama de estudantes universitárias. Porém, há uma demanda de pesquisas experimentais sobre os impactos da arte sobre a saúde física e mental de seus praticantes universitários, no intuito de conduzir e orientar os seus praticantes durante a realização dos eventos esportivos. Objetivo Verificar os efeitos da prática de Tai Chi Chuan sobre a saúde física das estudantes universitárias. Métodos Este estudo utilizou os métodos da literatura, experimental e estatística matemática, através da influência do Tai Chi Chuan estilo número 24 na saúde física das estudantes universitárias femininas em diferentes grupos. Resultados A pesquisa mostra que sob a intervenção desse estilo, com o mesmo conteúdo, intensidade e tempo de exercício, a saúde física de estudantes universitárias do sexo feminino no grupo de baixa aptidão física foi significativamente aprimorada. Conclusão Estudantes universitárias do sexo feminino no grupo de aptidão física média e alta melhoraram seus indicadores de saúde física devido ao seu pré-condicionamento físico. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción El Taichi Chuan es un arte marcial rico en características étnicas tradicionales chinas. Sus movimientos flexibles, libres y abiertos hacen que su práctica sea popular y apreciada por un amplio abanico de estudiantes universitarios. Sin embargo, existe una demanda de investigación experimental sobre los impactos del arte en la salud física y mental de sus practicantes universitarios, con el fin de conducir y guiar a sus practicantes durante los eventos deportivos. Objetivo Verificar los efectos de la práctica de Taichi Chuan en la salud física de estudiantes universitarios. Métodos Este estudio utilizó los métodos de la literatura, experimental y estadística matemática, a través de la influencia de Taichi Chuan estilo número 24 en la salud física de las estudiantes universitarias en diferentes grupos. Resultados La investigación demuestra que, bajo la intervención de este estilo, con el mismo contenido, intensidad y tiempo de ejercicio, mejoró significativamente la salud física de las estudiantes universitarias del grupo de baja forma física. Conclusión Las estudiantes universitarias del grupo de aptitud física media y alta mejoraron sus indicadores de salud física gracias a su preacondicionamiento físico. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
ABSTRACT
Climate is a dominant factor affecting the potential geographical distribution of species. Understanding the impact of climate change on the potential geographic distribution of species, which is of great significance to the exploitation, utilization, and protection of resources, as well as ecologically sustainable development. Betula platyphylla Suk. is one of the most widely distributed temperate deciduous tree species in East Asia and has important economic and ecological value. Based on 231 species distribution data points of Betula platyphylla Suk. in China and 37 bioclimatic, soil, and topography variables (with correlation coefficients < 0.75), the potential geographical distribution pattern of Betula platyphylla Suk. under Representative Concentration Pathway (RCP) climate change scenarios at present and in the 2050s and 2070s was predicted using the MaxEnt model. We analyzed the main environmental variables affecting the distribution and change of suitable areas and compared the scope and change of suitable areas under different climate scenarios. This study found: (1) At present, the main suitable area for Betula platyphylla Suk. extends from northeastern to southwestern China, with the periphery area showing fragmented distribution. (2) Annual precipitation, precipitation of the warmest quarter, mean temperature of the warmest quarter, annual mean temperature, and precipitation of the driest month are the dominant environmental variables that affect the potential geographical distribution of Betula platyphylla Suk. (3) The suitable area for Betula platyphylla Suk. is expected to expand under global warming scenarios. In recent years, due to the impact of diseases and insect infestation, and environmental damage, the natural Betula platyphylla Suk. forest in China has gradually narrowed. This study accurately predicted the potential geographical distribution of Betula platyphylla Suk. under current and future climate change scenarios, which can provide the scientific basis for the cultivation, management, and sustainable utilization of Betula platyphylla Suk. resources.
Subject(s)
Climate Change , Ecosystem , Betula , ChinaABSTRACT
Scientific experimental racks are an indispensable supporter in space stations for experiments with regard to meeting different temperature and humidity requirements. The diversity of experiments brings enormous challenges to the thermal control system of racks. This paper presents an indirect coupling thermal control single-phase fluid loop system for scientific experimental racks, along with fuzzy incremental control strategies. A dynamic model of the thermal control system is built, and three control strategies for it, with different inputs and outputs, are simulated. A comparison of the calculated results showed that pump speed and outlet temperature of the cold plate branch are, respectively, the best choice for the control variable and controlled variable in the controller. It showed that an indirect coupling thermal control fluid loop system with a fuzzy incremental controller is feasible for the thermal control of scientific experimental racks in space stations.
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Methyl 5-N-acetyl-7-O-acetyl-4-azido-2,3-didehydro-2,4-dideoxy-neuraminic acid (4azido-Neu2en5,7 Ac(2)1Me) was synthesized regioselectively starting from 4azido-Neu2en5Ac1Me in high yield. The transformation of 4azido-Neu2en5,7Ac(2)1Me to the corresponding thermodynamically stable 4azido-Neu2en5,9Ac(2)1Me via intramolecular acetyl migration was confirmed by single-crystal X-ray diffraction analysis. The proposed rearrangement mechanism is discussed.
Subject(s)
Carbohydrates/chemistry , Carbohydrates/chemical synthesis , N-Acetylneuraminic Acid/analogs & derivatives , Carbohydrate Conformation , Crystallography, X-Ray , Hydrolysis , Models, Molecular , N-Acetylneuraminic Acid/chemical synthesis , N-Acetylneuraminic Acid/chemistryABSTRACT
Mol-ecules of the title compound, C(10)H(12)FNO(3), are nearly planar considering all non-H atoms with a mean deviation of 0.0288â Å. Mol-ecules are linked through inter-molecular N-Hâ¯O and N-Hâ¯F hydrogen bonds.
