ABSTRACT
A comparison of the volatile compounds in Rhizomes Curcumae (Ezhu) and Radix Curcumae (Yujin) was undertaken using gas chromatography-mass spectrometry (GC-MS). Ultrasonic extraction and GC-MS methods were developed for the simultaneous determination of five sesquiterpenes, namely, α-pinene, ß-elemene, curcumol, germacrone and curdione, in Ezhu and Yunjin. Good linearity (r>0.999) and high inter-day precision were observed over the investigated concentration ranges. The validated method was successfully used for the simultaneous determination of five sesquiterpenes in Ezhu and Yujin. The quantitative method can be effectively used to evaluate and monitor the quality of Chinese curcuma in clinical use.
ABSTRACT
OBJECTIVE: To analyze the chemical constituents of essential oil extracted from Rhizoma Curcumae. METHODS: Headspace-GC/MS was employed to prepare essential oil from rhizome curcuma, with TR-5 quartz capillary column, extraction temperature 90 degrees C, hold 30 min, injection volume 1.5 mL. CONCLUSION: A rapid, simple and reliable method using HS-GC/MS was developed to analyze 79 volatile components, which can be an alternative for quality control for TCMs such as Rhizome Curcuma.
Subject(s)
Curcuma/chemistry , Gas Chromatography-Mass Spectrometry/methods , Oils, Volatile/analysis , Plants, Medicinal/chemistry , Terpenes/analysis , Oils, Volatile/chemistry , Oils, Volatile/isolation & purification , Plant Oils/analysis , Plant Oils/chemistry , Plant Oils/isolation & purification , Rhizome/chemistry , Solvents , Steam , Technology, Pharmaceutical/methods , VolatilizationABSTRACT
A reliable and rapid method based on rapid-resolution liquid chromatography-diode array detection (RRLC-DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) has been developed for the isolation and characterization of multiple constituents in the root of Stellera chamaejasme L., which was extracted by sonication with methanol in an optimized procedure. Separation of the multiple constituents was achieved on an Agilent Zorbax XDB-C18 (50x3.0 mm i.d.; 1.8 microm) column using a gradient elution at a flow rate of 0.4 mL/min. The detection wavelength was 210 nm. Mass spectra were acquired in both positive and negative modes. A formula database of the known chemical constituents in the root of Stellera chamaejasme L. was established by an Agilent software. Twenty-two obvious peaks appeared in the total ion chromatogram and nine of them were characterized by TOF/MS. The RRLC-DAD and ESI-TOF/MS method with ultrasonic extraction would be useful for rapid and effective characterization of chemical constituents in the root of Stellera chamaejasme L.
Subject(s)
Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/analysis , Plant Roots/chemistry , Spectrometry, Mass, Electrospray Ionization/methods , Tandem Mass Spectrometry/methods , Thymelaeaceae/chemistry , Coumarins/analysis , Coumarins/chemistry , Diterpenes/analysis , Diterpenes/chemistry , Drugs, Chinese Herbal/chemistry , Flavonoids/analysis , Flavonoids/chemistry , Glycosides/analysis , Glycosides/chemistry , Lasers, Semiconductor , Lignans/analysis , Lignans/chemistry , Methanol/chemistry , Molecular Weight , Reproducibility of Results , Spectrophotometry, Ultraviolet , StereoisomerismABSTRACT
AIM: To determine calycosin-7-O-beta-D-glucoside, astragaloside IV and formononetin in Radix Astragali and other relative samples by HPLC-MS. METHODS: HPLC was carried out with Agilent 1100LC/MSD, equipped with Agilent Zorbax SB C18 column (250 mm x 4.6 mm ID, 5 microm) and mass spectrum detector. The mobile phase (CH3CN-H2O) was eluted in gradient mode. RESULTS: The calibration curves of calycosin-7-O-beta-D-glucoside, astragaloside IV and formononetin were linear in the range of 0.03 - 1.21 microg x mL(-1), 0.35 - 13.86 microg x mL(-1) and 0.38 - 15.22 microg x mL(-1), respectively. These recoveries of samples were from 95% to 105% with RSD less than 1.5%. CONCLUSION: The method was employed to analyse 25 samples of Radix Astragali and other relative samples, including Radix Astragali slice, Radix Astragali Preparata, Hedysarum polybotrys Hand. -Mazz, Astragalus ernestii Comb. The contents of three constituents vary greatly because of the species, place of collection and season of harvesting. This method could apply to evaluate the quality of Radix Astragali and it is simple, sensitive and reliable.
Subject(s)
Astragalus propinquus/chemistry , Glucosides/analysis , Isoflavones/analysis , Plants, Medicinal/chemistry , Saponins/analysis , Triterpenes/analysis , Astragalus Plant/chemistry , China , Chromatography, High Pressure Liquid/methods , Ecosystem , Plant Roots/chemistry , Reproducibility of Results , Seasons , Species Specificity , Spectrometry, Mass, Electrospray Ionization/methodsABSTRACT
AIM: To investigate the chemical composition of the root of Salvia przewalskii Maxim. METHODS: Compounds were isolated by silica gel column chromatography. Structures of these compounds were elucidated by spectral analysis (EI-MS, FAB-MS, 1HNMR, 13CNMR, 1H-1H COSY, 1H-13C COSY, HMBC, NOESY) and phytochemical properties. RESULTS: Eight compounds were isolated and identified as: tanshinone II-A (I), crypotanshinone (II), przewaquinone A (III), sugiol (IV), ursolic acid (V), 2 alpha, 3 alpha-dihydroxy urs-12-ene-28-acid (VI), oleanolic acid (VII), and neo-przewaquinone A (VIII). CONCLUSION: Compound VIII is a new compound, and compound II, IV, V, VI and VII are isolated from this plant for the first time.
