Floral diversity and pollination strategies of three rheophytic Schismatoglottideae (Araceae).

Homoplastic evolution of 'unique' morphological characteristics in the Schismatoglottideae - many previously used to define genera - prompted this study to compare morphology and function in connection with pollination biology for Aridarum nicolsonii, Phymatarum borneense and Schottarum sarikeense. Aridarum nicolsonii and P. borneense extrude pollen through a pair of horned thecae while S. sarikeense sheds pollen through a pair of pores on the thecae. Floral traits of spathe constriction, presence and movement of sterile structures on the spadix, the comparable role of horned thecae and thecae pores, the presence of stamen-associated calcium oxalate packages, and the timing of odour emission are discussed in the context of their roles in pollinator management. Pollinators for all investigated species were determined to be species of Colocasiomyia (Diptera: Drosophilidae).

Invasive differences among Porphyromonas gingivalis strains from healthy and diseased periodontal sites.

BACKGROUND AND OBJECTIVE: The purpose of this study was to determine any difference between Porphyromonas gingivalis isolates from periodontally healthy sites as compared to those from diseased sites with respect to the ability to invade host cells. MATERIAL AND METHODS: Subgingival plaque samples were obtained from periodontally healthy and diseased sites using paper points. P. gingivalis colonies were isolated and tested, using an antibiotic protection assay, for their ability to invade KB cells. P. gingivalis 381 and Escherichia coli MC1061 were used as controls. RESULTS: Mean values of 16.79 +/- 0.86 x 10(3) colony-forming units/mL and 26.14 +/- 2.11 x 10(3) colony-forming units/mL were observed in invasion assays for isolates from periodontally healthy and diseased sites, respectively. P. gingivalis present in diseased sites had significantly greater invasive abilities than strains isolated from healthy sites. No statistical difference was noted between male or female subjects concerning the degree of invasion; isolates from diseased sites from both genders had significantly greater invasion abilities than those from healthy sites. A significant correlation was found between the increased invasive capabilities of P. gingivalis isolates vs. an increased probing depth. CONCLUSION: The increased invasion noted with P. gingivalis isolates from diseased sites vs. healthy sites, and the increased invasive capabilities with increasing probing depth, indicate that P. gingivalis isolates have a varying ability to invade host cells in the periodontal pocket.

Linear correlation between axial and lateral bone mineral density of lumbar vertebrae.

The placement of most spinal interbody devices is above the cancellous region of the vertebral bodies. Dual X-ray absorptiometry (DXA) scans of patients are usually taken from the anterior-posterior or the lateral projection. DXA scans taken in vitro from the axial direction would most closely indicate the bone mineral density (BMD) of the bone at this region. So far, in vitro studies have not been conducted to determine the correlation between axial and lateral scans. Because lateral DXA BMD scans are noninvasive and have low radiation dosage, they could be used as a predictive tool to determine the quality of cancellous bone at the centrum of the vertebral bodies, where spinal interbody devices are commonly placed.

Cyclic pamidronate infusion improves bone mineralisation and reduces fracture incidence in osteogenesis imperfecta.

UNLABELLED: A prospective open study was performed to determine the efficacy and safety of pamidronate in improving bone mineralisation and reducing fracture incidence in osteogenesis imperfecta (OI). Intravenous pamidronate was administered at 1.5 mg/kg bi-monthly to six children with OI, over 12-23 months. The number of fractures decreased from median of 3 (range 1-12) to 0 fractures/year (range 0-4) (P<0.05). After 12 months of treatment, there was significant improvement in areal bone mineral density (BMD) z-scores of the lumbar spine from median of -2.40 (range -3.20 to -1.67) to -1.90 (range -2.38 to -0.91) (P<0.05) and in the volumetric BMD which increased from median of 0.095 to 0.146 g/cm3 (P<0.05). Urine N-telopeptide levels (bone resorption marker) decreased from a median of 461.5 bone collagen equivalent/creatinine (BCE/Cr) (range 129-721 BCE/Cr) to 223.5 BCE/Cr (range 107-312 BCE/Cr) (P<0.05) and serum alkaline phosphatase (ALP) (bone formation marker) from a median of 230.0 U/l (range 148-305 U/l) to 133.5 U/l (range 79-233 U/l) (P<0.05), reflecting reduced bone turnover. This may represent a net reduction in bone resorption and provides a biochemical explanation for the increase in bone mineralisation. Height standard deviation scores were not affected and there were no significant adverse effects. CONCLUSION: 1 year cyclical pamidronate is effective and safe in improving bone mineralisation and reducing fracture incidence in osteogenesis imperfecta.

Periprosthetic bone remodelling after cementless total hip replacement. A prospective comparison of two different implant designs.

In a prospective study of 14 patients undergoing total hip replacement we have used dual-energy X-ray absorptiometry (DEXA) to investigate remodelling of the bone around two different designs of cementless femoral prosthesis. The bone mineral density (BMD) was measured at 12-weekly intervals for a year. Eight patients (group A) had a stiff, collarless implant and six (group B) a flexible isoelastic implant. Patients in group A showed a decrease in BMD from 14 weeks after operation. By 12 months, the mean loss in BMD was 27%, both medially and laterally to the proximal part of the implant. Those in group B showed an overall increase in BMD which reached a mean of 12.6% on the lateral side of the distal portion of the implant. Our results support the current concepts of the effects of stem stiffness and flexibility on periprosthetic remodelling.

Effect of femoral rotation on bone mineral density measurements with dual energy X-ray absorptiometry.

Precision in femoral neck scans with dual energy X-ray absorptiometry (DXA) is affected by variability in positioning and subsequent repositioning of the femur for repeated scans. To study the in vitro effect of femoral rotation on the bone mineral density (BMD), four fresh-frozen cadaveric femurs were fixed in a specially designed jig which allows for rotation of the femurs. BMD measurements of the femurs were done in neutral position (0 degrees) i.e., with the femoral neck axis parallel to the surface of the couch and at 15 degrees, 30 degrees, and 45 degrees of internal and external rotation. In vivo precision of the femoral neck scan was determined in five normal male subjects. The scans were first done with the legs positioned using the manufacturer's foot block. Five scans were performed, with repositioning, on the left hip of each subject. The procedure was then repeated with the legs positioned using a custom-designed positioning jig to minimize the rotation of the hips during a scan. In the in vitro study, the femoral neck BMD value was minimum at neutral position (0 degrees) and increased when the femur was rotated internally or externally. In vivo precision error of the femoral neck scan was reduced by almost 50% with the use of the positioning jig when compared with the manufacturer's foot block. Femoral rotation was shown to have a significant effect on BMD measurements, and proper positioning of the femur during a scan can improve precision significantly.

Effect of decalcification on bone mineral content and bending strength of feline femur.

The relationships between bone mineral content (BMC), bone calcium, and bone strength were studied in fractionally demineralized feline femurs. In 44 pairs of cat femurs, the right bones were decalcified in ethylene diaminetetra acetic acid (EDTA) to 20%, 40%, 60%, 80%, and 100% of the mineral content of the intact left bone (= control). The bones were then loaded to failure, and maximum strength values were recorded. The data were then used to calculate the percentage strength of the right relative to the left femurs. A correlation coefficient (r) of 0.970 was found between the percentage decalcification and percentage bending strength. A direct relationship (r = 0.876) was also observed between the total calcium extracted and total loss in BMC. The EDTA solutions were spot checked for protein content to determine if the organic matrices had been altered by demineralization. Protein was never detected. Nor did the demineralized tissues display histologic evidence of gross microscopic damage. This study has shown that in cat femurs, 20% decalcification led to about 35% loss in bending strength, and 60% decalcification caused 75% loss in strength. These values are significant as they highlight the importance of calcium to the strength of osteopenic bone.

Vertebral body index and bone mineral density in women with spinal fractures. 66 probands compared with controls.

The bone mineral density (BMD) was measured in 61 women with compression fractures of the spine and 66 normal pre-menopausal women. Radiographs of the lateral spine were also obtained and the vertebral body index (VBI) was measured in the region L2-L4. Women with spinal fractures had lower BMD and VBI values. Using both BMD and VBI, 3 regions of fracture risk were defined. All but 2 of the fracture patients were in the moderate- or high-risk region for fracture.