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2.
Nat Struct Biol ; 8(9): 761-5, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11524677

ABSTRACT

The first crystal structure of a protein, the Z alpha high affinity binding domain of the RNA editing enzyme ADAR1, bound to left-handed Z-DNA was recently described. The essential set of residues determined from this structure to be critical for Z-DNA recognition was used to search the database for other proteins with the potential for Z-DNA binding. We found that the tumor-associated protein DLM-1 contains a domain with remarkable sequence similarities to Z alpha(ADAR). Here we report the crystal structure of this DLM-1 domain bound to left-handed Z-DNA at 1.85 A resolution. Comparison of Z-DNA binding by DLM-1 and ADAR1 reveals a common structure-specific recognition core within the binding domain. However, the domains differ in certain residues peripheral to the protein-DNA interface. These structures reveal a general mechanism of Z-DNA recognition, suggesting the existence of a family of winged-helix proteins sharing a common Z-DNA binding motif.


Subject(s)
Adenosine Deaminase/chemistry , Conserved Sequence , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/metabolism , DNA/metabolism , Glycoproteins/chemistry , Glycoproteins/metabolism , Adenosine Deaminase/metabolism , Amino Acid Sequence , Animals , Crystallography, X-Ray , DNA/chemistry , DNA/genetics , Mice , Models, Molecular , Molecular Sequence Data , Nucleic Acid Conformation , Protein Conformation , Protein Structure, Tertiary , RNA-Binding Proteins , Sequence Alignment , Ultracentrifugation
3.
Proc Natl Acad Sci U S A ; 97(25): 13532-6, 2000 Dec 05.
Article in English | MEDLINE | ID: mdl-11087828

ABSTRACT

The Zalpha domain of human double-stranded RNA adenosine deaminase 1 binds specifically to left-handed Z-DNA and stabilizes the Z-conformation. Here we report spectroscopic and analytical results that demonstrate that Zalpha can also stabilize the left-handed Z-conformation in double-stranded RNA. Zalpha induces a slow transition from the right-handed A-conformation to the Z-form in duplex r(CG)(6), with an activation energy of 38 kcal mol(-1). We conclude that Z-RNA as well as Z-DNA can be accommodated in the tailored binding site of Zalpha. The specific binding of Z-RNA by Zalpha may be involved in targeting double-stranded RNA adenosine deaminase 1 for a role in hypermutation of RNA viruses.


Subject(s)
Adenosine Deaminase/metabolism , DNA/metabolism , RNA/metabolism , Adenosine Deaminase/chemistry , Circular Dichroism , Humans , Kinetics , Nucleic Acid Conformation , Protein Binding , RNA/chemistry , RNA-Binding Proteins , Spectrum Analysis, Raman
4.
J Biol Chem ; 275(35): 26828-33, 2000 Sep 01.
Article in English | MEDLINE | ID: mdl-10843996

ABSTRACT

The Zab domain of the editing enzyme ADAR1 binds tightly and specifically to Z-DNA stabilized by bromination or supercoiling. A stoichiometric amount of protein has been shown to convert a substrate of suitable sequence to the Z form, as demonstrated by a characteristic change in the CD spectrum of the DNA. Now we show that Zab can bind not only to isolated Z-forming d(CG)(n) sequences but also to d(CG)(n) embedded in B-DNA. The binding of Zab to such sequences results in a complex including Z-DNA, B-DNA, and two B-Z junctions. In this complex, the d(CG)(n) sequence, but not the flanking region, is in the Z conformation. The presence of Z-DNA was detected by cleavage with a Z-DNA specific nuclease, by undermethylation using Z-DNA sensitive SssI methylase, and by circular dichroism. It is possible that Zab binds to B-DNA with low affinity and flips any favorable sequence into Z-DNA, resulting in a high affinity complex. Alternatively, Zab may capture Z-DNA that exists transiently in solution. The binding of Zab to potential as well as established Z-DNA segments suggests that the range of biological substrates might be wider than previously thought.


Subject(s)
Adenosine Deaminase/metabolism , DNA-Binding Proteins/metabolism , DNA/metabolism , RNA Editing , Adenosine Deaminase/chemistry , Base Sequence , Circular Dichroism , DNA/chemistry , DNA-Binding Proteins/chemistry , Humans , Isomerism , Plasmids , RNA-Binding Proteins
5.
FEBS Lett ; 458(1): 27-31, 1999 Sep 10.
Article in English | MEDLINE | ID: mdl-10518927

ABSTRACT

The Z alpha domain of the human RNA editing enzyme double-stranded RNA deaminase I (ADAR1) binds to left-handed Z-DNA with high affinity. We found by analytical ultracentrifugation and CD spectroscopy that two Z alpha domains bind to one d(CG)3T4(CG)3 hairpin which contains a stem of six base pairs in the Z-DNA conformation. Both wild-type Z alpha and a C125S mutant show a mean dissociation constant of 30 nM as measured by surface plasmon resonance and analytical ultracentrifugation. Our data suggest that short (> or = 6 bp) segments of Z-DNA within a gene are able to recruit two ADAR1 enzymes to that particular site.


Subject(s)
Adenosine Deaminase/genetics , Adenosine Deaminase/metabolism , DNA/metabolism , RNA Editing , Binding Sites , Circular Dichroism , Dose-Response Relationship, Drug , Humans , Kinetics , Mutagenesis , Nucleic Acid Conformation , Protein Binding , RNA-Binding Proteins , Time Factors , Ultracentrifugation
6.
Proc Natl Acad Sci U S A ; 96(22): 12465-70, 1999 Oct 26.
Article in English | MEDLINE | ID: mdl-10535945

ABSTRACT

Double-stranded RNA deaminase I (ADAR1) contains the Z-DNA binding domain Zalpha. Here we report the solution structure of free Zalpha and map the interaction surface with Z-DNA, confirming roles previously assigned to residues by mutagenesis. Comparison with the crystal structure of the (Zalpha)(2)/Z-DNA complex shows that most Z-DNA contacting residues in free Zalpha are prepositioned to bind Z-DNA, thus minimizing the entropic cost of binding. Comparison with homologous (alpha+beta)helix-turn-helix/B-DNA complexes suggests that binding of Zalpha to B-DNA is disfavored by steric hindrance, but does not eliminate the possibility that related domains may bind to both B- and Z-DNA.


Subject(s)
Adenosine Deaminase/chemistry , Adenosine Deaminase/metabolism , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/metabolism , DNA/metabolism , Amino Acid Sequence , Binding Sites , Humans , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular Sequence Data , Protein Conformation , RNA-Binding Proteins , Sequence Homology, Amino Acid , Solutions
7.
Acta Crystallogr D Biol Crystallogr ; 55(Pt 7): 1362-4, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10393308

ABSTRACT

The proteolytically defined Z-DNA binding domain Za of human adenosine deaminase type 1 (hADAR1) has been crystallized in complex with the DNA oligomer d(TCGCGCG). The crystals were obtained from a solution containing ammonium sulfate as precipitating agent and belong to the tetragonal space group P4212. A complete diffraction data set has been collected to a resolution of 2.4 A. The unit-cell dimensions are a = b = 85.9, c = 71.3 A. A Raman spectrum of the complex indicates that the DNA in the complex adopts the left-handed Z conformation.


Subject(s)
Adenosine Deaminase/chemistry , DNA-Binding Proteins/chemistry , Adenosine Deaminase/metabolism , Binding Sites , Crystallization , Crystallography, X-Ray , DNA/metabolism , Humans , Protein Conformation , Spectrum Analysis, Raman
8.
J Biol Chem ; 274(27): 19081-6, 1999 Jul 02.
Article in English | MEDLINE | ID: mdl-10383410

ABSTRACT

Zab is a structurally defined protein domain that binds specifically to DNA in the Z conformation. It consists of amino acids 133-368 from the N terminus of human double-stranded RNA adenosine deaminase, which is implicated in RNA editing. Zab contains two motifs with related sequence, Zalpha and Zbeta. Zalpha alone is capable of binding Z-DNA with high affinity, whereas Zbeta alone has little DNA binding activity. Instead, Zbeta modulates Zalpha binding, resulting in increased sequence specificity for alternating (dCdG)n as compared with (dCdA/dTdG)n. This relative specificity has previously been demonstrated with short oligonucleotides. Here we demonstrate that Zab can also bind tightly to (dCdG)n stabilized in the Z form in supercoiled plasmids. Binding was assayed by monitoring cleavage of the plasmids using fusion nucleases, in which Z-DNA-binding peptides from the N terminus of double-stranded RNA adenosine deaminase are linked to the nuclease domain of FokI. A fusion nuclease containing Zalpha shows less sequence specificity, as well as less conformation specificity, than one containing Zab. Further, a construct in which Zbeta has been replaced in Zab with Zalpha, cleaves Z-DNA regions in supercoiled plasmids more efficiently than the wild type but with little sequence specificity. We conclude that in the Zab domain, both Zalpha and Zbeta contact DNA. Zalpha contributes contacts that produce conformation specificity but not sequence specificity. In contrast, Zbeta contributes weakly to binding affinity but discriminates between sequences of Z-DNAs.


Subject(s)
Adenosine Deaminase/metabolism , DNA-Binding Proteins/metabolism , DNA/metabolism , Deoxyribonucleases/metabolism , Base Sequence , Binding Sites , Deoxyribonucleases/genetics , Humans , Molecular Sequence Data , Nucleic Acid Conformation , RNA-Binding Proteins , Recombinant Fusion Proteins/metabolism , Substrate Specificity
9.
Science ; 284(5421): 1841-5, 1999 Jun 11.
Article in English | MEDLINE | ID: mdl-10364558

ABSTRACT

The editing enzyme double-stranded RNA adenosine deaminase includes a DNA binding domain, Zalpha, which is specific for left-handed Z-DNA. The 2.1 angstrom crystal structure of Zalpha complexed to DNA reveals that the substrate is in the left-handed Z conformation. The contacts between Zalpha and Z-DNA are made primarily with the "zigzag" sugar-phosphate backbone, which provides a basis for the specificity for the Z conformation. A single base contact is observed to guanine in the syn conformation, characteristic of Z-DNA. Intriguingly, the helix-turn-helix motif, frequently used to recognize B-DNA, is used by Zalpha to contact Z-DNA.


Subject(s)
Adenosine Deaminase/chemistry , DNA/metabolism , Adenosine Deaminase/metabolism , Amino Acid Sequence , Binding Sites , Crystallography, X-Ray , DNA/chemistry , Helix-Turn-Helix Motifs , Humans , Hydrogen Bonding , Models, Molecular , Molecular Sequence Data , Nucleic Acid Conformation , Protein Conformation , Protein Structure, Secondary , RNA-Binding Proteins , Substrate Specificity , Water/metabolism
10.
EMBO J ; 18(2): 470-9, 1999 Jan 15.
Article in English | MEDLINE | ID: mdl-9889202

ABSTRACT

RNA editing alters pre-mRNA through site-selective adenosine deamination, which results in codon changes that lead to the production of novel proteins. An enzyme that catalyzes this reaction, double-stranded RNA adenosine deaminase (ADAR1), contains two N-terminal Z-DNA-binding motifs, Zalpha and Zbeta, the function of which is as yet unknown. In this study, multidimensional NMR spectroscopy was used to show that the topology of Zalpha is alpha1beta1alpha2alpha3beta2beta3. Long-range NOEs indicate that beta1 and beta3 interact with each other. Site-directed mutagenesis was used to identify residues in alpha3, beta3 and the loop connecting beta2 to beta3 that affect Z-DNA binding. Also identified were 11 hydrophobic residues that are essential for protein stability. Comparison with known structures reveals some similarity between Zalpha and (alpha + beta) helix-turn-helix proteins, such as histone 5 and the family of hepatocyte nuclear factor-3 winged-helix-turn-helix transcription factors. Taken together, the structural and functional data suggest that recognition of Z-DNA by Zalpha involves residues in both the alpha3 helix and the C-terminal beta-sheet.


Subject(s)
Adenosine Deaminase/chemistry , Adenosine Deaminase/metabolism , DNA/metabolism , Adenosine Deaminase/genetics , Amino Acid Sequence , Binding Sites/genetics , Circular Dichroism , Escherichia coli/genetics , Helix-Turn-Helix Motifs , Humans , In Vitro Techniques , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Protein Conformation , Protein Structure, Secondary , RNA-Binding Proteins , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism
11.
J Biol Chem ; 274(5): 2899-906, 1999 Jan 29.
Article in English | MEDLINE | ID: mdl-9915827

ABSTRACT

Zalpha is a peptide motif that binds to Z-DNA with high affinity. This motif binds to alternating dC-dG sequences stabilized in the Z-conformation by means of bromination or supercoiling, but not to B-DNA. Zalpha is part of the N-terminal region of double-stranded RNA adenosine deaminase (ADAR1), a candidate enzyme for nuclear pre-mRNA editing in mammals. Zalpha is conserved in ADAR1 from many species; in each case, there is a second similar motif, Zbeta, separated from Zalpha by a more divergent linker. To investigate the structure-function relationship of Zalpha, its domain structure was studied by limited proteolysis. Proteolytic profiles indicated that Zalpha is part of a domain, Zab, of 229 amino acids (residues 133-361 in human ADAR1). This domain contains both Zalpha and Zbeta as well as a tandem repeat of a 49-amino acid linker module. Prolonged proteolysis revealed a minimal core domain of 77 amino acids (positions 133-209), containing only Zalpha, which is sufficient to bind left-handed Z-DNA; however, the substrate binding is strikingly different from that of Zab. The second motif, Zbeta, retains its structural integrity only in the context of Zab and does not bind Z-DNA as a separate entity. These results suggest that Zalpha and Zbeta act as a single bipartite domain. In the presence of substrate DNA, Zab becomes more resistant to proteases, suggesting that it adopts a more rigid structure when bound to its substrate, possibly with conformational changes in parts of the protein.


Subject(s)
Adenosine Deaminase/metabolism , DNA-Binding Proteins/metabolism , DNA/metabolism , Binding Sites , Cell Line , Chymotrypsin/metabolism , Circular Dichroism , Humans , Nucleic Acid Conformation , Protein Conformation , Protein Folding , Protein Structure, Secondary , RNA Editing , RNA-Binding Proteins , Repetitive Sequences, Amino Acid , Serine Endopeptidases/metabolism , Thermolysin/metabolism , Trypsin/metabolism
12.
Biochemistry ; 37(38): 13313-21, 1998 Sep 22.
Article in English | MEDLINE | ID: mdl-9748339

ABSTRACT

Double-stranded RNA adenosine deaminase (ADAR1) is an ubiquitous enzyme in metazoa that edits pre-mRNA changing adenosine to inosine in regions of double-stranded RNA. Zalpha, an N-terminal domain of human ADAR1 encompassing 76 amino acid residues, shows apparent specificity for the left-handed Z-DNA conformation adopted by alternating (dGdC) polymers modified by bromination or methylation, as well as for (dGdC)13 inserts present in supercoiled plasmids. Here, a combination of circular dichroism, fluorescence, and gel-retardation studies is utilized to characterize recombinant Zalpha peptide and to examine its interaction with DNA. Results from laser-Raman spectroscopy experiments provide direct evidence for the existence of Z-DNA in peptide-DNA complexes.


Subject(s)
Adenosine Deaminase/chemistry , DNA/chemistry , RNA Editing , Adenosine Deaminase/metabolism , Amino Acid Sequence , Animals , Chickens , Circular Dichroism , DNA/metabolism , Deoxycytidine/chemistry , Deoxyguanosine/chemistry , Hot Temperature , Humans , Macromolecular Substances , Molecular Sequence Data , Nucleic Acid Conformation , Peptides/chemistry , Polydeoxyribonucleotides/chemistry , Protein Conformation , Protein Denaturation , RNA-Binding Proteins , Spectrometry, Fluorescence , Spectrum Analysis, Raman
13.
Nucleic Acids Res ; 26(15): 3486-93, 1998 Aug 01.
Article in English | MEDLINE | ID: mdl-9671809

ABSTRACT

Z-DNA, the left-handed conformer of DNA, is stabilized by the negative supercoiling generated during the movement of an RNA polymerase through a gene. Recently, we have shown that the editing enzyme ADAR1 (double-stranded RNA adenosine deaminase, type 1) has two Z-DNA binding motifs, Zalpha and Zbeta, the function of which is currently unknown. Here we show that a peptide containing the Zalpha motif binds with high affinity to Z-DNA as a dimer, that the binding site is no larger than 6 bp and that the Zalpha domain can flip a range of sequences, including d(TA)3, into the Z-DNAconformation. Evidence is also presented to show that Zalpha and Zbeta interact to form a functional DNA binding site. Studies with atomic force microscopy reveal that binding of Zalpha to supercoiled plasmids is associated with relaxation of the plasmid. Pronounced kinking of DNA is observed, and appears to be induced by binding of Zalpha. The results reported here support a model where the Z-DNA binding motifs target ADAR1 to regions of negative supercoiling in actively transcribing genes. In this situation, binding by Zalpha would be dependent upon the local level of negative superhelicity rather than the presence of any particular sequence.


Subject(s)
Adenosine Deaminase/metabolism , DNA-Binding Proteins/metabolism , DNA/metabolism , Binding Sites , Humans , Microscopy, Atomic Force , Protein Binding , Protein Conformation , RNA-Binding Proteins
14.
Genetics ; 148(1): 233-42, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9475735

ABSTRACT

HeT-A, a major component of Drosophila telomeres, is the first retrotransposon proposed to have a vital cellular function. Unlike most retrotransposons, more than half of its genome is noncoding. The 3' end contains > 2.5 kb of noncoding sequence. Copies of HeT-A differ by insertions or deletions and multiple nucleotide changes, which initially led us to conclude that HeT-A noncoding sequences are very fluid. However, we can now report, on the basis of new sequences and further analyses, that most of these differences are due to the existence of a small number of conserved sequence subfamilies, not to extensive sequence change during each transposition event. The high level of sequence conservation within subfamilies suggests that they arise from a small number of replicatively active elements. All HeT-A subfamilies show preservation of two intriguing features. First, segments of extremely A-rich sequence form a distinctive pattern within the 3' noncoding region. Second, there is a strong strand bias of nucleotide composition: The DNA strand running 5' to 3' toward the middle of the chromosome is unusually rich in adenine and unusually poor in guanine. Although not faced with the constraints of coding sequences, the HeT-A 3' noncoding sequence appears to be under other evolutionary constraints, possibly reflecting its roles in the telomeres.


Subject(s)
Drosophila/genetics , Retroelements/genetics , Telomere/genetics , Animals , Conserved Sequence , Sequence Alignment
15.
Genetica ; 100(1-3): 73-84, 1997.
Article in English | MEDLINE | ID: mdl-9440260

ABSTRACT

Transposable elements are abundant in the genomes of higher organisms but are usually thought to affect cells only incidentally, by transposing in or near a gene and influencing its expression. Telomeres of Drosophila chromosomes are maintained by two non-LTR retrotransposons, HeT-A and TART. These are the first transposable elements with identified roles in chromosome structure. We suggest that these elements may be evolutionarily related to telomerase; in both cases an enzyme extends the end of a chromosome by adding DNA copied from an RNA template. The evolution of transposable elements from chromosomal replication mechanisms may have occurred multiple times, although in other organisms the new products have not replaced the endogenous telomerase, as they have in Drosophila. This is somewhat reminiscent of the oncogenes that have arisen from cellular genes. Perhaps the viruses that carry oncogenes have also arisen from cellular genetic systems.


Subject(s)
Drosophila/genetics , Evolution, Molecular , Retroelements , Telomere , Amino Acid Sequence , Animals , Molecular Sequence Data , Repetitive Sequences, Nucleic Acid , Sequence Homology, Amino Acid , Telomerase/genetics
16.
J Mol Evol ; 43(6): 572-83, 1996 Dec.
Article in English | MEDLINE | ID: mdl-8995054

ABSTRACT

A major component of Drosophila telomeres is the retrotransposon HeT-A, which is clearly related to other retrotransposons and retroviruses. This retrotransposon is distinguished by its exclusively telomeric location, and by the fact that, unlike other retrotransposons, it does not encode its own reverse transcriptase. HeT-A coding sequences diverge significantly, even between elements within the same genome. Such rapid divergence has been noted previously in studies of gag genes from other retroelements. Sequence comparisons indicate that the entire HeT-A coding region codes for gag protein, with regions of similarity to other insect retrotransposon gag proteins found throughout the open reading frame (ORF). Similarity is most striking in the zinc knuckle region, a region characteristic of gag genes of most replication-competent retroelements. We identify a subgroup of insect non-LTR retrotransposons with three zinc knuckles of the form: (1) CX2CX4HX4C, (2) CX2CX3HX4C, (3) CX2CX3HX6C. The first and third knuckles are invariant, but the second shows some differences between members of this subgroup. This subgroup includes HeT-A and a second Drosophila telomeric retrotransposon, TART. Unlike other gag regions, HeT-A requires a -1 frameshift for complete translation. Such frameshifts are common between the gag and pol sequences of retroviruses but have not before been seen within a gag sequence. The frameshift allows HeT-A to encode two polypeptides; this mechanism may substitute for the post-translational cleavage that creates multiple gag polypeptides in retroviruses. D. melanogaster HeT-A coding sequences have a polymorphic region with insertions/deletions of 1-31 codons and many nucleotide changes. None of these changes interrupt the open reading frame, arguing that only elements with translatable ORFs can be incorporated into the chromosomes. Perhaps HeT-A translation products act in cis to target the RNA to chromosome ends.


Subject(s)
DNA Transposable Elements , Drosophila Proteins , Drosophila/genetics , Gene Products, gag , Insect Proteins/genetics , Polymorphism, Genetic , Retroelements/genetics , Telomere/genetics , Amino Acid Sequence , Animals , Binding Sites , Blotting, Northern , Conserved Sequence , Drosophila melanogaster/genetics , Insect Proteins/metabolism , Molecular Sequence Data , Open Reading Frames , Peptide Fragments/genetics , Peptide Fragments/metabolism , Protein Biosynthesis , Sequence Homology, Amino Acid , Telomere/metabolism , Transcription, Genetic , Zinc/metabolism
17.
Trends Genet ; 12(2): 48-52, 1996 Feb.
Article in English | MEDLINE | ID: mdl-8851970

ABSTRACT

In Drosophila, chromosome ends (telomeres) are composed of telomere-specific transposable elements (the retroposons HeT-A and TART). These elements are a bona fide part of the cellular machinery yet have many of the hallmarks of retrotransposable elements and retroviruses, raising the possibility that parasitic transposable elements and viruses might have evolved from mechanisms that the cell uses to maintain its chromosomes. It is striking that Drosophila, the model organism for many discoveries in genetics, development and molecular biology (including the classical concept of telomeres), should prove to have chromosome ends different from the generally accepted model. Studies of these telomere-specific retrotransposable elements raise questions about conventional wisdom concerning not only telomeres, but also transposable elements and heterochromatin.


Subject(s)
Drosophila/genetics , Evolution, Molecular , Telomere/genetics , Animals , Base Sequence , Molecular Sequence Data , Retroelements
18.
Proc Natl Acad Sci U S A ; 92(16): 7550-4, 1995 Aug 01.
Article in English | MEDLINE | ID: mdl-7638229

ABSTRACT

A M(r) 140,000 protein has been purified from chicken lungs to apparent homogeneity. The protein binds with high affinity to a non-BNA conformation, which is most likely to the Z-DNA. The protein also has a binding site for double-stranded RNA (dsRNA). Peptide sequences from this protein show similarity to dsRNA adenosine deaminase, an enzyme that deaminates adenosine in dsRNA to form inosine. Assays for this enzyme confirm that dsRNA adenosine deaminase activity and Z-DNA binding are properties of the same molecule. The coupling of these two activities in a single molecule may indicate a distinctive mechanism of gene regulation that is, in part, dependent on DNA topology. As such, DNA topology, through its effects on the efficiency and extent of RNA editing may be important in the generation of new phenotypes during evolution.


Subject(s)
Adenosine Deaminase/metabolism , Adenosine Deaminase/genetics , Adenosine Deaminase/isolation & purification , Amino Acid Sequence , Animals , Chickens , DNA/chemistry , Humans , In Vitro Techniques , Lung/enzymology , Molecular Sequence Data , Nucleic Acid Conformation , RNA-Binding Proteins , Rats , Sequence Homology, Amino Acid , Substrate Specificity
19.
Nucleic Acids Symp Ser ; (33): 16-9, 1995.
Article in English | MEDLINE | ID: mdl-8643357

ABSTRACT

A Z-DNA binding protein of 140,000 M(r) has been purified from chicken lungs by sedimentation through 40%(w/w) sucrose and Z-DNA affinity chromatography. Specificity of the protein for Z-DNA was confirmed by competition with polyd(CG) that had been stabilized in the Z-DNA conformer by chemical bromination and also with a supercoiled plasmid that contains a Z-DNA-forming insert. In addition to a Z-DNA binding site, the protein also has a separate binding site for double-stranded RNA. Peptide sequence of the protein shows that it has high similarity to the RNA editing enzyme double-stranded RNA adenosine deaminase (dsRAD), which deaminates adenosine in dsRNA to form inosine. The Z-DNA binding protein has this enzymatic activity, confirming its identity to dsRAD. Recombinant human dsRAD also binds to Z-DNA. Z-DNA is stabilized in a sequence-dependent manner by negative supercoiling, which occurs in actively transcribed genes upstream to RNA polymerase. It is proposed that Z-DNA links editing to transcription by localizing dsRAD to a particular region of a gene and thus determines the efficiency with which an RNA is edited. The presence of Z-DNA forming elements in many genes raises the possibility that RNA editing by dsRAD is far more prevalent than is currently thought.


Subject(s)
Adenosine Deaminase/metabolism , DNA/metabolism , Adenosine Deaminase/chemistry , Adenosine Deaminase/isolation & purification , Animals , Binding Sites , Chickens , Chromatography, Affinity , DNA/chemistry , DNA/genetics , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/isolation & purification , DNA-Binding Proteins/metabolism , Humans , In Vitro Techniques , Male , Molecular Weight , Nucleic Acid Conformation , RNA Editing , RNA-Binding Proteins , Recombinant Proteins/metabolism , Salmon , Substrate Specificity , Transcription, Genetic
20.
Proc Natl Acad Sci U S A ; 90(8): 3339-42, 1993 Apr 15.
Article in English | MEDLINE | ID: mdl-8475077

ABSTRACT

A protein (Z alpha) that appears to be highly specific for the left-handed Z-DNA conformer has been identified in chicken blood nuclear extracts. Z alpha activity is measured in a band-shift assay by using a radioactive probe consisting of a (dC-dG)35 oligomer that has 50% of the deoxycytosines replaced with 5-bromodeoxycytosine. In the presence of 10 mM Mg2+, the probe converts to the Z-DNA conformation and is bound by Z alpha. The binding of Z alpha to the radioactive probe is specifically blocked by competition with linear poly(dC-dG) stabilized in the Z-DNA form by chemical bromination but not by B-form poly(dC-dG) or boiled salmon-sperm DNA. In addition, the binding activity of Z alpha is competitively blocked by supercoiled plasmids containing a Z-DNA insert but not by either the linearized plasmid or by an equivalent amount of the parental supercoiled plasmid without the Z-DNA-forming insert. Z alpha can be crosslinked to the 32P-labeled brominated probe with UV light, allowing us to estimate that the minimal molecular mass of Z alpha is 39 kDa.


Subject(s)
Blood Cells/metabolism , Cell Nucleus/metabolism , DNA-Binding Proteins/blood , DNA/metabolism , Animals , Chickens , Cross-Linking Reagents , DNA-Binding Proteins/isolation & purification , Electrophoresis, Polyacrylamide Gel , Molecular Weight , Oligodeoxyribonucleotides/chemical synthesis , Oligodeoxyribonucleotides/metabolism , Oligonucleotide Probes
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