ABSTRACT
Monitoring both the trace element and main element content of drugs forms part of their quality control. Chromium, selenium and molybdenum were determined in therapeutic diet samples by inductively coupled plasma/mass spectrometry (ICP-MS) and electrothermal atomic absorption spectrometry (ET-AAS). Samples were digested by high pressure microwave digestion or by ashing in oven. ICP-MS measurements have demonstrated that the chromium determination in liquid therapeutic diet should be estimated on the basis of 53Cr. In solid samples in some cases the value for Cr was elevated in comparison with the Cr content found by ET-AAS. The content of selenium can be determined on the basis of 77Se or 82Se after appropriate interference correction. Molybdenum content was determined on the basis of 95Mo. Control measurements were made by ET-AAS. For quality assurance purposes some of the samples were analyzed by a control laboratory.
Subject(s)
Chromium/analysis , Diet , Food, Formulated/analysis , Molybdenum/analysis , Selenium/analysis , Chlorophyta/chemistry , Powders , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Atomic , Nicotiana/chemistryABSTRACT
As, Ca, Cd, Cu, Fe, Mg, Mn, Pb and Zn were determined by ICP-MS and ET-AAS in preparations made from peppermint and nettle leaves after microwave high pressure digestion with nitric acid. In preparation from nettle leaves the Ca content was more than three times higher than in preparations made from peppermint leaves. Only very small differences (less than 10%) were observed for all the other elements. In all the samples investigated the content of inorganic elements were below the WHO limit (where existing). Variation coefficients ranged from 0.68% to 10.5% for ICP-MS measurements and from 1.48% to 10.0% for ET-ASS.
Subject(s)
Mentha piperita/chemistry , Urtica dioica/chemistry , Calibration , Elements , Mass Spectrometry , Microwaves , Plant Leaves/chemistry , Spectrophotometry, AtomicABSTRACT
Controlling of elements content in drugs is indispensable for drug quality evaluation. Zinc was selected for study because zinc contents in drugs span a wide range. Numerous multi-mineral preparations used and nutritional supplements to make up for the microelemental deficiencies in humans and animals, contain less than 0.001% zinc, i.e., trace amounts of zinc. On the other hand, external application drugs such as ointments and suppositories usually contain more than 0.1% of zinc. Zinc was determined out in Technilab's "Hemacort HC" ointments and suppositories, in Nattermann's "Dolovit Multimineral" tablets, and in Richter Pharma's "Minerasol" parenteral veterinary solution. These preparations were selected for investigation not only in view of differentiated zinc contents, but also because of the various drug forms and various procedures involved in sample preparation procedures. The tablets were dissolved with the aid of microwave energy, the ointment and suppositories were extracted, and the parenteral solution was diluted with water or with and aqueous 1% nitric acid. Atomic absorption spectrometry with graphite-tube (AAS), inductively coupled plasma mass spectrometry (ICP-MS), adsorptive stripping voltammetry (AdSV), square-wave voltammetry (SWV), and differential pulse polarography (DPP) were used for the determinations. Zinc was determined within concentration ranges of 20-40 ppb, 0.5-20 ppb, and 10-50 ppb by AAS, ICP-MS and AdSV, respectively. In the AAS and ICP-MS, zinc recoveries were contained within the limits of 101.8-114.2% and 111.0-114.2% respectively. The errors of determination (RSD) were 3.37-5.74% in AAS, 0.28-4.47% in ICP-MS and 0.19-2.50% in the electrochemical method.
Subject(s)
Zinc/analysis , Calibration , Electrochemistry , Indicators and Reagents , Mass Spectrometry , Ointments , Spectrophotometry, AtomicABSTRACT
A method for the determination of the content of Zn, Mg and Ca in Therapeutical Nutrients by flameless atomic absorption spectrometry is described. This elements were determined after microwave digestion of the samples. To establish optimum conditions for mineralization procedure, different parameters such as mass of the sample, amounts and ratios of used acids, times and power of microwave digestion were tested. The results showed that microwave digestion of samples allows to perform complete mineralization of the sample in short time and with no losses of the determined element.
