Theoretical grounds and experimental confirmation of the antiviral effect of the preparation Ukrain.

The preparation Ukrain was studied in order to confirm its theoretically substantiated properties in comparison with amitozin as its structural and functional analogue, and two other functional analogues, namely methisazone (against herpesvirus and poxvirus) and remantadine (against grippevirus). The experiments were carried out on albino Swiss mice of either sex weighing initially 18-20 g. They were kept at room temperature (20-21 degrees C) on a natural day-night cycle, were housed in groups of ten per cage and had free access to food and water. The Ukrain preparation's activity as concerns grippe virus of A type (England) 42/72 (H3N2) was determined in ovo and in vivo, the treatment beginning 2 h after infection. The control animals, infected by appropriate virus dose, were injected with placebo. For the activities of the remedies investigated, our estimate was based on the median duration of life in the mice of the subexperimental and control groups (duration of experiments 14 days), the degree of intensity of pulmonary tissue changes, and the difference in median HR titres. The totality of Chelidonium majus alkaloids, thiophosphamide (the alkalizing agent), the sum of alkaloids modified by thiotepa, and some other alkaloids as their alkalized derivatives, all showed a statistically reliable inhibiting effect. The most significant biological activity was demonstrated by Ukrain as concerns the models of viral processes, and by the sum of alkaloids and amitozin as concerns the models of associated infections. This coincides with the results of biological assessment of modified products (1, 3, 5) with metabolites, alkaloids in particular, or alkaline substances.

Biological activity of some thiophosphamide derivatives of alkaloids with respect to influenza virus.

Certain newly synthesized compounds belonging to the class of alkaloid ethylenimines, and those known but not subjected to the test for antiviral selection, were studied. The in vitro, in ovo and in vivo experiments detected the substances (Ukrain, amitozin, colchamine-thiotepa, thiotepa) with developed antiviral effect relative to the influenza A/H3 N2/virus strain Port Chalmers 1/73. In the work we used, 1, 10 and 100 ID50 in 0,1 ml. The virus was injected at 1, 10 and 100 EID50 per embryo. The assessment in vivo was carried out on mice of 16-18 g weight. The LD50 for mice having been determined by the probit-analysis method, therapeutic concentrations were used at 1/10 of the LD50. The preparations were found to possess an inhibitory action for the virus, along with insignificant toxicity for the systems studied. Antigrippe action was revealed by the T alkilic agent, in this case thiophosphamide, in all systems. The comparative study of the main component of the alkaloid sum, and of 2 products of their modification (amitozin and Ukrain), showed promise great for creating remedies using such methods and materials. Especially worthy of attention was the Ukrain preparation, which may be recommended as a potential antiviral remedy with the aim of widening the choice of preparations with an antiviral action.