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1.
Heliyon ; 10(1): e23970, 2024 Jan 15.
Article in English | MEDLINE | ID: mdl-38268593

ABSTRACT

The workforce is becoming increasingly heterogeneous in terms of age, gender, race/ethnicity, education, values, cognition, and culture. Thus, team diversity management is regarded as an important development strategy that organizations can use to gain advantages. However, in the diversity literature, empirical studies investigating the effects of cognitive diversity on creativity have not yielded conclusive findings. This has called into question the importance of team cognitive diversity. To address this, we investigate when and how team cognitive diversity fosters individual creativity. Drawing on the categorization-elaboration model (CEM) and literature on Chaxu climate, we develop a multilevel mediated moderation model in which the team Chaxu climate is treated as the moderator and team knowledge sharing is treated as the mediator. Using two-wave paired data collected from 46 teams and 368 members, we find that Chaxu climate mitigates the positive effect of team cognitive diversity on team knowledge sharing. In turn, team knowledge sharing mediates the interaction effect between team cognitive diversity and Chaxu climate on individual creativity. Our study facilitates a shift from an automatic-oriented lens to a contingent-oriented lens by identifying a new contingent factor and advances research on the underlying mechanisms by identifying a new process factor. Ultimately, this study enriches our knowledge on the function of cognitive diversity in the field of business strategy.

2.
Insects ; 14(12)2023 Dec 01.
Article in English | MEDLINE | ID: mdl-38132593

ABSTRACT

Insects' gut microbiota plays a crucial role in their host selection, adaptation, and plasticity. This study explored the impact of gut bacteria on the adaptation of host selection under different stresses (diverse feeding preferences and no feeding preferences). The seventh instar E. signifer larvae were artificially transferred from the most-selected host E. grandis × E. urophylla (Es) to more preferred hosts, M. apelta (Ma), as well as the non-preferred host, B. papyrifera (Bp). We then obtained the larval gut of three different feeding preference hosts. The gut bacterial DNA was sequenced and analyzed based on 16S rRNA. There were significant differences in the composition of dominant gut bacteria between Es with Ma and Bp, but without significant differences between Ma and Bp. In the process, Burkholderia and Microbacillus with degrading pesticides had significant changes, and Enterococcus with insect gut probiotics also had significant changes. The presence of enterococcus may be one of the main causes of intestinal microbiota changes before and after host transfer. Notably, when the feeding of E. signifer changes, the complex connections that exist between gut bacteria also change. Additionally, there was a negative correlation between the feeding preferences of E. signifer and the metabolic functions of their gut bacteria. This study provided a theoretical basis for the prediction and use of gut bacteria to interfere with the feeding of E. signifer.

3.
Life (Basel) ; 13(12)2023 Nov 27.
Article in English | MEDLINE | ID: mdl-38137865

ABSTRACT

Endoclita signifer is a prominent wood-boring insect species in eucalyptus plantations in Guangxi, China, causing significant ecological and economic damage. A novel approach to controlling the challenging wood-boring pest involves disrupting the olfactory communication between insects and the volatile compounds emitted by plants. To identify the olfactory proteins contributing to host selection based on 11 GC-EAD-active volatiles from eucalyptus leaves and to discover the highly expressed olfactory proteins, we conducted a study on the antennal transcriptomes of adult E. signifer and screened key olfactory proteins in the antennae. We identified a total of 69 olfactory proteins. When compared to the larval transcriptomes, the antennal transcriptome of adult E. signifer revealed the presence of 17 new odorant-binding proteins (OBPs), including 2 pheromone-binding proteins (PBPs), 7 previously unreported chemosensory proteins (CSPs), 17 new odorant receptors (ORs), 4 new gustatory receptors (GRs), 11 novel ionotropic receptors (IRs), and 2 sensory neuron membrane proteins (SNMPs). Through the phylogenetic tree of OBPs and ORs, we identified EsigPBP2 and EsigPBP3 as two of the three PBPs, designated EsigOR13 as EsigOrco, and recognized EsigOR10 and EsigOR22 as the newly discovered EsigPRs in E. signifer. In the adult antennae, the expression levels of EsigGOBP14, EsigGOBP13, EsigOBP14, EsigOBP17, EsigCSP14, and EsigOR16 were notably high, indicating that these proteins could be pivotal in binding to plant volatiles.

5.
ChemSusChem ; 16(13): e202300143, 2023 Jul 07.
Article in English | MEDLINE | ID: mdl-37055348

ABSTRACT

Utilization of renewable raw materials as feedstock defossilizes industrial manufacturing while subsequent carbon capture reduces carbon footprint. We applied this concept to design a new pyrolysis-based process for synthesis of biogenic multi-walled carbon nanotubes (MWCNTs) and H2 from biomass. It was demonstrated that the conversion of hydrocarbon compounds in pyrolysis gas into MWCNTs and H2 is detrimentally influenced by accompanied CO2 released from biomass decomposition. Capturing CO2 with a calcium sorbent upgraded the pyrolysis gas into a suitable gaseous precursor for downstream production of MWCNTs and H2 -rich gas. Furthermore, the results suggest that CO2 capture with the sorbent has a potential to outperform a liquid alkaline scrubber owing to avoided liquid organic waste generation, sorbent regenerability and higher H2 recovery from biomass pyrolysis gas.


Subject(s)
Carbon Dioxide , Nanotubes, Carbon , Biomass , Hydrogen , Pyrolysis
6.
Environ Technol ; 44(25): 3897-3910, 2023 Nov.
Article in English | MEDLINE | ID: mdl-35543664

ABSTRACT

The remediation of dyes in wastewater using activated carbon produced from sewage sludge pyrolysis char (PYC) is an environmentally friendly and sustainable process. However, traditional activators can cause corrosion of the processing facility, thereby increasing the costs of waste disposal. Here, activated carbons were prepared from sewage sludge PYC, and the effects of activation conditions (different activators, temperature and time, and char:activator mass ratio) on their specific surface areas and adsorption of iodine and methylene blue (MB; model dye) were studied. The results showed that a value of 952 m2/g could be attained for the specific surface area and values of 882 and 162 mg/g for the adsorption of iodine and MB, respectively, by heating PYC with KHCO3 (PYC- KHCO3: 1:2 w/w) for 60 min at 800 ℃. Compared with activation by KOH, the adsorption of MB using PYC-KHCO3 was slightly lower but the yield was 13.7% higher. Optimization of the activation process using surface response modelling indicated that sensitivity of three key factors to the adsorption of iodine and MB followed the order: Mass ratio > temperature > time. Systematic investigation of the effects of time, pH and temperature on the removal of MB by the activated carbon revealed that adsorption conformed to the Langmuir model and pseudo-second-order kinetics. The proposed mechanisms of MB adsorption involved ion exchange, functional group complexation and physical/π-π interactions. This study provides a basis for the efficient remediation of dyes in wastewater using activated carbon prepared from sustainable sewage sludge PYC and green chemistry.


Subject(s)
Iodine , Water Pollutants, Chemical , Water Purification , Sewage , Wastewater , Charcoal , Coloring Agents , Water Purification/methods , Adsorption , Methylene Blue , Kinetics
7.
Front Pharmacol ; 13: 1028046, 2022.
Article in English | MEDLINE | ID: mdl-36353487

ABSTRACT

Background: Euodiae Fructus, a well-known herbal medicine, is widely used in Asia and has also gained in popularity in Western countries over the last decades. It has known side effects, which have been observed in clinical settings, but few studies have reported on its cardiotoxicity. Methods: In the present study, experiments using techniques of untargeted metabolomics clarify the hazardous effects of Euodiae Fructus on cardiac function and metabolism in rats in situations of overdosage and unsuitable syndrome differentiation. In vitro assays are conducted to observe the toxic effects of evodiamine and rutaecarpine, two main chemical constituents of Euodiae Fructus, in H9c2 and neonatal rat cardiomyocytes (NRCMs), with their signaling mechanisms analyzed accordingly. Results: The cardiac cytotoxicity of evodiamine and rutaecarpine in in vivo experiments is associated with remarkable alterations in lactate dehydrogenase, creatine kinase, and mitochondrial membrane potential; also with increased intensity of calcium fluorescence, decreased protein expression of the cGMP-PKG pathway in H9c2 cells, and frequency of spontaneous beat in NRCMs. Additionally, the results in rats with Yin deficiency receiving a high-dosage of Euodiae Fructus suggest obvious cardiac physiological dysfunction, abnormal electrocardiogram, pathological injuries, and decreased expression of PKG protein. At the level of endogenous metabolites, the cardiac side effects of overdose and irrational usage of Euodiae Fructus relate to 34 differential metabolites and 10 metabolic pathways involving among others, the purine metabolism, the glycerophospholipid metabolism, the glycerolipid metabolism, and the sphingolipid metabolism. Conclusion: These findings shed new light on the cardiotoxicity induced by Euodiae Fructus, which might be associated with overdose and unsuitable syndrome differentiation, that comes from modulating the cGMP-PKG pathway and disturbing the metabolic pathways of purine, lipid, and amino acid. Continuing research is needed to ensure pharmacovigilance for the safe administration of Chinese herbs in the future.

8.
ACS Omega ; 7(40): 35911-35923, 2022 Oct 11.
Article in English | MEDLINE | ID: mdl-36249376

ABSTRACT

This study was performed to investigate the herb-drug interactions (HDIs) of citrus herbs (CHs), which was inspired by the "grapefruit (GF) juice effect". Based on network analysis, a total of 249 components in GF and 159 compounds in CHs exhibited great potential as active ingredients. Moreover, 360 GF-related genes, 422 CH-related genes, and 111 genes associated with drug transport and metabolism were collected, while 25 and 26 overlapping genes were identified. In compound-target networks, the degrees of naringenin, isopimpinellin, apigenin, sinensetin, and isoimperatorin were higher, and the results of protein-protein interaction indicated the hub role of UGT1A1 and CYP3A4. Conventional drugs such as erlotinib, nilotinib, tamoxifen, theophylline, venlafaxine, and verapamil were associated with GF and CHs via multiple drug transporters and drug-metabolizing enzymes. Remarkably, GF and CHs shared 48 potential active compounds, among which naringenin, tangeretin, nobiletin, and apigenin possessed more interactions with targets. Drug metabolism by cytochrome P450 stood out in the mutual mechanism of GF and CHs. Molecular docking was utilized to elevate the protein-ligand binding potential of naringenin, tangeretin, nobiletin, and apigenin with UGT1A1 and CYP3A4. Furthermore, in vitro experiments demonstrated their regulating effect. Overall, this approach provided predictions on the HDIs of CHs, and they were tentatively verified through molecular docking and cell tests. Moreover, there is a demand for clinical and experimental evidence to support the prediction.

9.
Int J Mol Sci ; 23(16)2022 Aug 17.
Article in English | MEDLINE | ID: mdl-36012538

ABSTRACT

Endoclita signifer larvae show olfactory recognition towards volatiles of eucalyptus trunks and humus soils. Further, EsigGOBP1 was identified through larval head transcriptome and speculated as the main odorant-binding proteins in E. signifer larvae. In this study, the highest expression of EsigGOBP1 was only expressed in the heads of 3rd instar larvae of E. signifer, compared with the thorax and abdomen; this was consistent with the phenomenon of habitat transfer of 3rd instar larvae, indicating that EsigGOBP1 was a key OBP gene in E. signifer larvae. Results of fluorescence competition binding assays (FCBA) showed that EsigGOBP1 had high binding affinities to eight GC-EAD active ligands. Furthermore, screening of key active odorants for EsigGOBP1 and molecular docking analysis, indicated that EsigGOBP1 showed high binding activity to alpha-phellandrene in 3rd instar larvae of E. signifer. Conformational analysis of the EsigGOBP1-alpha-phellandrene complex, showed that MET49 and GLU38 were the key sites involved in binding. These results demonstrated that EsigGOBP1 is a key odorant-binding protein in E. signifer larvae, which recognizes and transports eight key volatiles from eucalyptus trunk, especially the main eucalyptus trunks volatile, alpha-phellandrene. Taken together, our results showed that EsigGOBP1 is involved in host selection of E. signifer larvae, which would aid in developing EsigGOBP1 as molecular targets for controlling pests at the larval stage.


Subject(s)
Lepidoptera , Receptors, Odorant , Animals , Cyclohexane Monoterpenes , Insect Proteins/genetics , Larva/metabolism , Lepidoptera/metabolism , Molecular Docking Simulation , Protein Binding , Receptors, Odorant/metabolism
10.
Nature ; 605(7909): 225, 2022 05.
Article in English | MEDLINE | ID: mdl-35538364
11.
IEEE J Biomed Health Inform ; 26(4): 1582-1590, 2022 04.
Article in English | MEDLINE | ID: mdl-35196250

ABSTRACT

Early detection and identification of malignant thyroid nodules, a vital precursory to the treatment, is a difficult task even for experienced clinicians. Many Computer-Aided Diagnose (CAD) systems have been developed to assist clinicians in performing this task on ultrasonic images. Learning-based CAD systems for thyroid nodules generally accommodate both nodule detection/ segmentation and fine-grained classification for its malignancy, and prior researches often treat aforementioned tasks in separate stages, leading to additional computational costs. In this paper, we utilize an online class activation mapping (CAM) mechanism to guide the network to learn discriminative features for identifying thyroid nodules in ultrasound images, called CAM attention network. It takes nodule masks as localization cues for direct spatial attention of the classification module, thereby avoiding isolated training for classification. Meanwhile, we propose a deformable convolution module to add offsets to the regular grid sampling locations in the standard convolution, guiding the network to capture more discriminative features of nodule areas. Furthermore, we use a generative adversarial network (GAN)to ensure reliable deformations of nodules from the deformable convolution module. Our proposed CAM attention network has already achieved the 2nd place in the classification task of TN-SCUI 2020, a MICCAI 2020 Challenge with the largest set of thyroid nodule ultrasound images according to our knowledge. The further inclusion of our proposed GAN-guided deformable module allows for capturing more fine-grained features between benign and malignant nodules, and further improves the classification accuracy to a new state-of-the-art level.


Subject(s)
Thyroid Nodule , Humans , Thyroid Nodule/diagnostic imaging , Ultrasonography/methods
12.
Langmuir ; 38(4): 1422-1431, 2022 Feb 01.
Article in English | MEDLINE | ID: mdl-35034443

ABSTRACT

Emulsion-templated, hydrophilic-oleophobic porous materials are promising for the removal of a small amount of water from oil-water mixtures, but the maneuver and complete collection of these porous materials are challenging. Herein, we report the fabrication of magnetic, hydrophilic-oleophobic polyHIPE composites from reactive Fe3O4 nanoparticle-stabilized high internal phase emulsions through simultaneous bulk polymerization of water-soluble monomers and interface-catalyzed polycondensation of 1H,1H,2H,2H-perfluorooctyltriethoxysilane. The resulting composites were hydrophilic-oleophobic, with water droplets rapidly absorbed (within 20 s), and exhibited designable magnetic responsiveness. The hydrophilicity-oleophobicity enabled water to be removed through selective absorption from oil-water mixtures (including surfactant-stabilized water-in-oil emulsions), with a high separation rate over 99%. The magnetic-responsiveness enabled both the dry and the swollen composites to be maneuvered in a remote and contactless manner and to be fully collected. Therefore, the magnetic, hydrophilic-oleophobic polyHIPE composites are excellent candidates for the removal of water from water-oil mixtures with complete collection.

13.
Soft Matter ; 17(42): 9653-9663, 2021 Nov 03.
Article in English | MEDLINE | ID: mdl-34633025

ABSTRACT

Macroporous materials templated using high internal phase emulsions (HIPEs) are promising for various applications. To date, new strategies to create emulsion-templated porous materials and to tune their properties (especially wetting properties) are still highly required. Here, we report the fabrication of macroporous polymers from oil-in-water HIPEs, bereft of conventional monomers and crosslinking monomers, by simultaneous ring-opening polymerization and interface-catalyzed condensation, without heating or removal of oxygen. The resulting macroporous polymers showed drying condition-dependent wetting properties (e.g., hydrophilicity-oleophilicity from freezing drying, hydrophilicity-oleophobicity from vacuum drying, and amphiphobicity from heat drying), densities (from 0.019 to 0.350 g cc-1), and compressive properties. Hydrophilic-oleophilic and amphiphobic porous polymers turned hydrophilic-oleophobic simply by heating and protonation, respectively. The hydrophilic-oleophobic porous polymers could remove a small amount of water from oil-water mixtures (including surfactant-stabilized water-in-oil emulsions) by selective absorption and could remove water-soluble dyes from oil-water mixtures. Moreover, the transition in wetting properties enabled the removal of water and dyes in a controlled manner. The feature that combines simply preparation, tunable wetting properties and densities, robust compression, high absorption capacity (rate) and controllable absorption makes the porous polymers to be excellent candidates for the removal of water and water-soluble dyes from oil-water mixtures.

14.
J Environ Manage ; 296: 113247, 2021 Oct 15.
Article in English | MEDLINE | ID: mdl-34265665

ABSTRACT

European Union (EU) has set ambitious energy and climate targets for the EU Member States. Some EU Member States (MS) confirmed to attain 100% of renewables in power generation by 2050 and implement carbon-free economy target. The purpose of this study is to assess and compare the avoided external costs of electricity generation due to the penetration of renewables in the Baltic States by implementing EU energy and climate targets for 2020, 2030 and 2050. The switching to renewables has significant social benefits for the EU member states, as fossil fuel burning is linked with the climate change and emission of classical air pollutants (SO2, NOx, particulate matter, NH3, NMVOC), which can have various negative impacts on the human health. The analysis and forecasts of external costs of power generation by 2050 in the selected countries, i.e., the Baltic States, has shown that Estonia is able to achieve the highest avoided external costs and highest health benefits from renewable promotion due to the oil shale that is dominating in the power generation structure. The main input of this study performed in the Baltic States is to show how subsidies for renewables correspond to the reduction of external costs of power generation and to provide important policy implications for these countries. Moreover, this can be an example for other countries how to assess the benefits of renewables support.


Subject(s)
Air Pollutants , Air Pollutants/analysis , Climate Change , Electricity , Fossil Fuels , Humans , Particulate Matter/analysis
15.
Materials (Basel) ; 13(23)2020 Nov 29.
Article in English | MEDLINE | ID: mdl-33260482

ABSTRACT

The steam oxidation behavior of three heterogeneous HR3C alloys was investigated at 650 °C comparatively. After a long-term oxidation process for 1000 h, the results demonstrated that the commercial HR3C alloy already exhibited a high oxidation resistance. However, the spallation resistance of the oxide scale was low during the initial oxidation period. The addition of a moderate amount of Nb into the alloy (1#HR3C) increased the oxidation resistance of the alloy. In addition, the improvement of the microstructural stability was substantially influenced by solid solution strengthening and fine grain strengthening. However, the addition of excessive Nb could significantly affect the growth model of the oxide scale and negatively affect the oxidation performance and microstructural evolution of the alloy (2#HR3C).

16.
Article in English | MEDLINE | ID: mdl-32707758

ABSTRACT

Implementation of strict policies for mitigating climate change has a direct impact on public health as far as the external health costs of electricity generation can be reduced, thanks to the reduction of emission of typical pollutants by switching to cleaner low carbon fuels and achieving energy efficiency improvements. Renewables have lower external health costs due to the lower life cycle emission of typical air pollutants linked to electricity generation, such as SO2, NOx, particulate matter, NH3, or NMVOC (Non-methane volatile organic compounds), which all appear to have serious negative effects on human health. Our case study performed in the Baltic States analyzed the dynamics of external health costs in parallel with the dynamics of the main health indicators in these countries: life expectancy at birth, mortality rates, healthy life years, self-perceived health, and illness indicators. We employed the data for external health costs retrieved from the CASES database, as well as the health statistics data compiled from the EUROSTAT database. The time range of the study was 2010-2018 due to the availability of consistent health indicators for the EU Member States. Our results show that the decrease of external health costs had a positive impact on the increase of the self-perceived good health and reduction of long-standing illness as well as the decrease of infant death rate. Our conclusions might be useful for other countries as well as for understanding the additional benefits of climate change mitigation policies and tracking their positive health impacts. The cooperation initiatives on clean energy and climate change mitigation between countries like One Belt One Road initiative by the Chinese government can also yield additional benefits linked to the public health improvements.


Subject(s)
Electricity , Air Pollutants/analysis , Air Pollution/analysis , Baltic States , Health Care Costs , Particulate Matter/analysis
17.
Psychiatr Danub ; 30(1): 26-34, 2018 Mar.
Article in English | MEDLINE | ID: mdl-29546855

ABSTRACT

BACKGROUND: It has been suggested that individual job characteristics have a significant impact on job burnout, and the process is subject to the regulation of demographic variables. However, the influence path of job characteristics on job burnout is still a "black box". SUBJECTS AND METHODS: On the basis of a systematic literature review by employing Pub Med, Science Direct, Web of Science, Google Scholar, CNKI and Scopus for required information with the several keywords "Job burnout", "Emotion regulation", "Personality traits", and "Psychological stress", in this study, an improved mine rescue workers-oriented job demands-resources (JD-R) model was put forward. Then, a novel analysis framework, to explore the impact of job characteristics on job burnout from the view of emotion regulation theory, was proposed combining the personality trait theory. RESULTS: This study argues that job burnout is influenced by job demands through expressive suppression and by job resources through cognitive reappraisal respectively. Further more, job demands and job resources have the opposite effects on job burnout through the "loss-path" caused by job pressure and the "gain-path" arised from job motivation, respectively. Extrovert personality traits can affect the way the individual processes the information of work environment and then how individual further adopts emotion regulation strategies, finally resulting in indirectly affecting the influence path of mine rescue workers' job characteristics on job burnout. CONCLUSIONS: This present study can help managers to realize the importance of employees' psychological stress and job burnout problems. The obtained conclusions provide significant decision-making references for managers in intervening job burnout, managing emotional stress and mental health of employees.


Subject(s)
Burnout, Professional/psychology , Emotional Adjustment , Emotional Intelligence , Job Description , Models, Psychological , Occupational Stress/psychology , Workload/psychology , Adult , Burnout, Professional/prevention & control , Character , Female , Humans , Job Satisfaction , Male , Personnel Management , Young Adult
18.
Iran J Public Health ; 45(10): 1247-1260, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27957431

ABSTRACT

BACKGROUND: With the increased range of medical insurance coverage in China, the proportion of medical expenditure shouldered by individuals is declining. The problem is the rapidly growing scale of medical expenditures challenges the sustainability of medical insurance funds. METHODS: This study used the Heckman selection model, survival analysis, and ordered probit model to evaluate the effect of medical insurance on the expenditures in outpatient and inpatient health care, survival time, and improvement of self-rated health of test subjects, respectively. RESULTS: Medical insurance exerts a differential effect on the expenditures in outpatient and inpatient health care. On average, the expenditures in outpatient and inpatient health care of test subjects participating in premium health insurance plans increased by 38.6% and 72.6%, respectively. Participation in medical insurance plans exhibits no significant correlation with the survival time of test subjects, but their self-rated health shows a significant correlation (P < 0.01). CONCLUSION: Although medical insurance does not significantly reduce mortality or prolong the survival time of test subjects, it improves their health status. This study suggests that the Chinese government should eliminate deductible medical insurance payments and utilize medical resources on minor ailment treatment and disease prevention to improve the health status of people.

19.
Iran J Public Health ; 43(11): 1478-85, 2014 Nov.
Article in English | MEDLINE | ID: mdl-26060714

ABSTRACT

A detailed study and analysis of previous research has been carried out to illustrate the relationships between a range of environmental emergencies, and their effects on the emotional state of the rescuers involved in responding to them, by employing Pub Med, Science Direct, Web of Science, Google Scholar, CNKI and Scopus for required information with the several keywords "emergency rescue", "occupational safety", "natural disaster", "emotional management". The effect of the rescuers' emotion on their occupational safety and immediate and long-term emotional behavior is then considered. From these considerations, we suggested four research propositions related to the emotional effects at both individual and group levels, and to the responsibilities of emergency response agencies in respect of ensuring the psychological and physical occupational safety of rescuers during and after environmental emergencies. An analysis framework is proposed which could be used to study the influence paths of these different aspects of emotional impact on a range of occupational safety issues for rescue workers. The authors believe that the conclusions drawn in this paper can provide a useful theoretical reference for decision-making related to the management and protection of the occupational safety of rescuers responding to natural disasters and environmental emergencies.

20.
J Ethnopharmacol ; 120(3): 452-7, 2008 Dec 08.
Article in English | MEDLINE | ID: mdl-18951966

ABSTRACT

AIM OF THE STUDY: To evaluate the anti-fibrotic effects of BJ-JN (a traditional Chinese formulation) in CCl(4)-induced liver fibrosis in rats. MATERIALS AND METHODS: BJ-JN (0.5, 1.0, 2.0 g/kg) was administrated via gavage once a day starting from the fifth weeks after the CCl(4) treatment for subsequent 9 weeks. Evaluated with liver and spleen index, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), nitric oxide (NO), hepatic malondialdehyde (MDA) content and superoxide dismutase (SOD) activity, as well as with histopathologic changes of liver. The proliferation and collagen synthesis of primary hepatic stellate cells (HSCs) from normal, model and BJ-JN (2.0 g/kg) treatment rats were examined with (3)H-TdR and (3)H-Pro uptake assay, respectively. RESULTS: BJ-JN (0.5, 1.0, 2.0 g/kg) effectively reduced the elevated levels of liver and spleen index, serum ALT, AST, NO, HA, and hepatic MDA contents, enhance the reduced hepatic SOD activity in CCl(4)-treated rats. The histopathological analysis suggested that BJ-JN obviously alleviated the degree of liver fibrosis induced by CCl(4). The proliferation and collagen synthesis of HSC isolated from BJ-JN (2.0 g/kg) treatment rats were remarkably inhibited. CONCLUSIONS: Those results suggest BJ-JN has a protective and therapeutic effect on liver fibrosis induced by CCl(4), which might be associated with its anti-oxidative activity, inhibitory activity on HSC proliferation and collagen synthesis.


Subject(s)
Carbon Tetrachloride Poisoning/drug therapy , Drugs, Chinese Herbal/therapeutic use , Hepatic Stellate Cells/drug effects , Liver Cirrhosis/drug therapy , Liver Cirrhosis/prevention & control , Animals , Carbon Tetrachloride/administration & dosage , Carbon Tetrachloride/toxicity , Carbon Tetrachloride Poisoning/complications , Drugs, Chinese Herbal/pharmacology , Hepatic Stellate Cells/pathology , Liver/drug effects , Liver/pathology , Liver Cirrhosis/chemically induced , Liver Cirrhosis/pathology , Liver Function Tests , Male , Medicine, Chinese Traditional , Random Allocation , Rats , Rats, Sprague-Dawley , Spleen/drug effects
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