Sequentially suspended 3D bioprinting of multiple-layered vascular models with tunable geometries forin vitromodeling of arterial disorders initiation.

As the main precursor of arterial disorders, endothelial dysfunction preferentially occurs in regions of arteries prone to generating turbulent flow, particularly in branched regions of vasculatures. Although various diseased models have been engineered to investigate arterial pathology, producing a multiple-layered vascular model with branched geometries that can recapitulate the critical physiological environments of human arteries, such as intercellular communications and local turbulent flows, remains challenging. This study develops a sequentially suspended three-dimensional bioprinting (SSB) strategy and a visible-light-curable decellularized extracellular matrix bioink (abbreviated as 'VCD bioink') to construct a biomimetic human arterial model with tunable geometries. The engineered multiple-layered arterial models with compartmentalized vascular cells can exhibit physiological functionality and pathological performance under defined physiological flows specified by computational fluid dynamics simulation. Using different configurations of the vascular models, we investigated the independent and synergetic effects of cellular crosstalk and abnormal hemodynamics on the initiation of endothelial dysfunction, a hallmark event of arterial disorder. The results suggest that the arterial model constructed using the SSB strategy and VCD bioinks has promise in establishing diagnostic/analytic platforms for understanding the pathophysiology of human arterial disorders and relevant abnormalities, such as atherosclerosis, aneurysms, and ischemic diseases.

Remaining Useful Life Prediction for Two-Phase Nonlinear Degrading Systems with Three-Source Variability.

Recently, the estimation of remaining useful life (RUL) for two-phase nonlinear degrading devices has shown rising momentum for ensuring their safe and reliable operation. The degradation processes of such systems are influenced by the temporal variability, unit-to-unit variability, and measurement variability jointly. However, current studies only consider these three sources of variability partially. To this end, this paper presents a two-phase nonlinear degradation model with three-source variability based on the nonlinear Wiener process. Then, the approximate analytical solution of the RUL with three-source variability is derived under the concept of the first passage time (FPT). For better implementation, the offline model parameter estimation is conducted by the maximum likelihood estimation (MLE), and the Bayesian rule in conjunction with the Kalman filtering (KF) algorithm are utilized for the online model updating. Finally, the effectiveness of the proposed approach is validated through a numerical example and a practical case study of the capacitor degradation data. The results show that it is necessary to incorporate three-source variability simultaneously into the RUL prediction of the two-phase nonlinear degrading systems.

Short Circuiting Transfer, Formation, and Microstructure of Ti-6Al-4V Alloy by External Longitudinal Magnetic Field Hybrid Metal Inert Gas Welding Additive Manufacturing.

In this work, the external longitudinal magnetic field hybrid metal inert gas welding (M-MIG) additive manufacturing method is employed to produce the Ti-6Al-4V alloy part. The effect of process parameters on the droplet transfer formation and microstructure of the part was studied by a high-speed camera, optical microscope, and electron backscattered diffraction. The results showed that a typical short-circuiting transfer was obtained with the wire feeding speed of 2 m/min-4 m/min. An external longitudinal magnetic field had an obvious effect on the arc shape. The uniform formation of the deposition layer was obtained with the wire feeding speed of 4 m/min. The width of M-MIG deposition layer was greater than that of the MIG, and the width of M-MIG deposition layer was increased with the increase of the magnetic excitation current. The microstructure of the deposition layer was mainly comprised of acicular martensite α' and massive martensite αm. In addition, the ß grain size in the M-MIG was less than that of the MIG. The average microhardness of the MIG deposition layer was 281.6 HV, which was less than that of M-MIG.

A Novel Unified Data Modeling Method for Equipment Lifecycle Integrated Logistics Support.

Integrated logistics support (ILS) is of great significance for maintaining equipment operational capability in the whole lifecycle. Numerous segments and complex product objects exist in the process of equipment ILS, which gives ILS data multi-source, heterogeneous, and multidimensional characteristics. The present ILS data cannot satisfy the demand for efficient utilization. Therefore, the unified modeling of ILS data is extremely urgent and significant. In this paper, a unified data modeling method is proposed to solve the consistent and comprehensive expression problem of ILS data. Firstly, a four-tier unified data modeling framework is constructed based on the analysis of ILS data characteristics. Secondly, the Core unified data model, Domain unified data model, and Instantiated unified data model are built successively. Then, the expressions of ILS data in the three dimensions of time, product, and activity are analyzed. Thirdly, the Lifecycle ILS unified data model is constructed, and the multidimensional information retrieval methods are discussed. Based on these, different systems in the equipment ILS process can share a set of data models and provide ILS designers with relevant data through different views. Finally, the practical ILS data models are constructed based on the developed unified data modeling software prototype, which verifies the feasibility of the proposed method.

Investigation of braided stents in curved vessels in terms of "Dogbone" deformation.

"Dogbone" deformation that the diameters of two ends are larger than the middle diameter of the stent under the effect of the balloon expanding, is one of the important standards to evaluate the mechanical properties of vascular stents. It is a huge challenge to simulate and evaluate the "Dogbone" behaviors of braided stents in the curved vessels. In this study, the key work was to investigate the "Dogbone" deformations of braided stents in the curved vessels by designing main parameters including strut diameter, braiding angle, and the circumferential number of unit cell. Based on the "Dogbone" stents in the curved vessels, the impact of "Dogbone" on the fatigue properties of braided stents was analyzed under the pulsatile effect of vessels. The influence of "Dogbone" stents on stress distribution of vascular walls was studied. To evaluate the "Dogbone" behaviors of stents in the curved vessels, the calculation method of "Dogbone" was improved by calculating the centerline and the bus bar of the curved vessels. Braided stents with various parameters (strut diameter t = 100,125 and 152 µm, braiding angle α = 30, 40 and 50°, the circumferential number of unit cell N = 8, 10, and 12) were designed respectively. Numerical simulation method was used to mimic the "Dogbone" deformation after stent expansion. The results showed that strut diameter and braiding angle had more influence on "Dogbone" deformations than the circumferential number of unit cell. "Dogbone" deformation could adversely affect fatigue performance and vascular walls.

Multi-Stream Convolutional Neural Networks for Rotating Machinery Fault Diagnosis under Noise and Trend Items.

In recent years, rotating machinery fault diagnosis methods based on convolutional neural network have achieved much success. However, in real industrial environments, interfering signals are unavoidable, which may reduce the accuracy of fault diagnosis seriously. Most of the current fault diagnosis methods are of single input type, which may lead to the information contained in the vibration signal not being fully utilized. In this study, theoretical analysis and comprehensive comparative experiments are completed to investigate the time domain input, frequency domain input, and two types of time-frequency domain input. Based on this, a new fault diagnosis model, named multi-stream convolutional neural network, is developed. The model takes the time domain, frequency domain, and time-frequency domain images as input, and it automatically fuses the information contained in different inputs. The proposed model is tested based on three public datasets. The experimental results suggested that the model achieved pretty high accuracy under noise and trend items without the help of signal separation algorithms. In addition, the positive implications of multiple inputs and information fusion are analyzed through the visualization of learned features.

A simple and sensitive AuNPs-based colorimetric aptasensor for specific detection of azlocillin.

A new colorimetric biosensor for specific detection of azlocillin was developed by using DNA aptamer as recognition element and unmodified gold nanoparticles (AuNPs) as colorimetric indicator. In the absence of azlocillin, the AuNPs were protected by the aptamer and stabilized at high NaCl concentrations, displaying a red solution. In the presence of azlocillin, the aptamer reacts specifically with azlocillin, resulting in the aggregation of AuNPs and an apparent red to blue color change. The characteristic change can be easily observed by the naked eye and quantitatively detected by an ultraviolet-visible (UV-Vis) spectrometer. Under the optimal conditions, the absorbance variation at 522 nm (ΔA522) of AuNPs changed proportionally with increasing concentration of azlocillin, which exhibited a linear relationship in the concentration range of 50 nM to 500 nM, with a detection limit of 11.6 nM. Furthermore, the aptasensor was successfully used to detect azlocillin in milk and tap water samples, with recoveries ranging from 97.64% to 102.21% and a relative standard deviation (RSD) less than 3.81%.

Structural Design of Vascular Stents: A Review.

Percutaneous Coronary Intervention (PCI) is currently the most conventional and effective method for clinically treating cardiovascular diseases such as atherosclerosis. Stent implantation, as one of the ways of PCI in the treatment of coronary artery diseases, has become a hot spot in scientific research with more and more patients suffering from cardiovascular diseases. However, vascular stent implanted into vessels of patients often causes complications such as In-Stent Restenosis (ISR). The vascular stent is one of the sophisticated medical devices, a reasonable structure of stent can effectively reduce the complications. In this paper, we introduce the evolution, performance evaluation standards, delivery and deployment, and manufacturing methods of vascular stents. Based on a large number of literature pieces, this paper focuses on designing structures of vascular stents in terms of "bridge (or link)" type, representative volume unit (RVE)/representative unit cell (RUC), and patient-specific stent. Finally, this paper gives an outlook on the future development of designing vascular stents.

Investigation on Morphology and Mechanical Properties of Rod Units in Lattice Structures Fabricated by Selective Laser Melting.

Selective laser melting (SLM) fabrication of lattice structures has attracted considerable interest due to its many immanent advantages, such as high specific strength. A wide variety of lattice structures have been designed and fabricated. However, as a vital prerequisite for design optimization, a clear relation between the process constraint of SLM and the apparent properties of the fabricated lattice structure has received much less attention. Therefore, this work systematically investigates the characterization and preformation of rod units, which are the basic components of lattice structures, so as to evaluate the SLM manufacturability of lattice structures. A series of rod units with different inclination angles and diameters were fabricated by SLM. Their morphology and mechanical properties were measured by scanning electron microscope observation and a tensile test, respectively. The inclination angle was found to have significant effects on profile error and little effect on mechanical properties. The higher the inclination angle, the larger the profile error. The characteristic diameter had no significant correlation with profile errors and mechanical properties. Based on systematic studies, a formula is proposed to evaluate the cross-sectional area of the fabricated rod units and further estimate their load capacity. This has important implications for optimizing the design of lattice structures fabricated by SLM.

Risk Factors and Scoring System of Cage Retropulsion after Posterior Lumbar Interbody Fusion: A Retrospective Observational Study.

OBJECTIVE: To investigate risk factors of cage retropulsion after posterior lumbar interbody fusion (PLIF) in China and to establish a scoring system of cage retropulsion. METHODS: The retrospective analysis was based on two hospital databases. The medical data records of posterior lumbar interbody fusion with cage retropulsion were selected from August 2009 to August 2019. Inclusion and exclusion criteria were set in advance. Risk factors including patients' baseline demographics (age, gender, operation diagnosis time difference), preoperative neurological symptoms, whether the fusion involves single or double segments, screw type, intraoperative compression, preoperative bone mineral density, whether there are neurological symptoms before surgery, whether there is urine dysfunction before surgery, disease type, complete removal of the endplate, and patient's education level. The research endpoint was the retropulsion of fusion cages. The Kaplan-Meier (K-M) method was used to analyze potential risk factors, and multivariate Cox regression was used to identify independent risk factors (P < 0.05). The Statistical Package for the Social Sciences (version 22.0; SPSS, IBM, Chicago, IL, USA) software was used for statistical analysis, and univariate analysis was used to screen out the factors related to cage retropulsion. All independent risk factors were included to predict the survival time of the retropulsion of cage. RESULTS: This study included a total of 32 patients with PLIF between 2009 to 2019. All patients were residents of China. Univariate analysis showed that there were 13 patients over 60 years old and 19 patients under 60 years old. There were 20 male patients and 12 female patients. The surgical diagnosis time was seven patients within 1 month, 17 patients within 1 to 3 months, and eight patients over 3 months. The disease type was 18 cases of lumbar disc herniation, 10 cases of lumbar spinal stenosis, four cases of lumbar spondylolisthesis. The fusion segment was 18 cases of single segment, 14 cases of double segment. The intraoperative compression was seven cases of compression, 25 cases of no compression. The preoperative bone mineral density was 10 cases of low density, 18 cases of normal, four cases of osteoporosis. The screw type was 27 cases of universal screw, five cases of one-way screw. Preoperative neurological symptoms were found in 25 cases and not in seven cases. Preoperative urination dysfunction occurred in 8 cases, whereas 24 cases did not have this dysfunction. The endplate was completely removed in 10 cases and not in 22 cases. Education level was nine cases of primary school education, 10 cases of secondary school, 13 cases of university level. Cox regression analysis showed that intraoperative pressure (hazard ratio [HR] = 4.604, P = 0.015) and complete removal of the endplate (HR = 0.205, P = 0.027) are associated with the time of cage retropulsion. According to the HR of each factor, the scoring rules were formulated, and the patients were divided into the low-risk group, moderate-risk group, and high-risk group according to the final score. The three median survival times of the three groups were 66 days in the low-risk group, 55 days in the moderate-risk group, and 45 days in the high-risk group, with statistical significance (P < 0.05). CONCLUSION: Intraoperative pressure and complete removal of the intraoperative endplate can be helpful to evaluating the expected time of cage retropulsion in patients with PLIF, and this clinical model guided the selection of postoperative prevention and follow-up treatment.

Bamboo grid versus polyvinyl chloride as packing material in cooling tower: Energy efficiency and environmental impact assessment.

As an abundant and fast-growing biomass, bamboo can be used as construction materials owing to its desirable physical and mechanical properties, environmentally friendly features, and alternative to replace toxic and hazardous wastes in industrial processing. In this study, grid material made from bamboo (termed 'bamboo grid') was developed and compared to commercially used polyvinyl chloride (PVC) as packing material in cooling towers; PVC packing has drawbacks such as fouling, deposit buildup, low durability, and is harmful to environments. The cooling capacity, energy efficiency and environmental impact of bamboo grid packing were evaluated via life cycle assessment (LCA), particularly the cumulative energy demand (CED) and the Building for Environmental and Economic Sustainability (BEES). Although the thermal performance of the PVC packing was found higher than that of the bamboo grid packing, the bamboo grid packing showed improved resistance characteristic, recording a total saving of 529.2 tons of standard coal during a six-month field test in a real thermal power generation plant. LCA results revealed that the utilization of bamboo-grid packing to replace PVC packing in cooling towers reduced total CED from 3420 MJ to 561 MJ per functional unit, achieving 6 times reduction. A desirable reduction ranging from 1.5 to 10.5 times was also recorded for the BEES indices. This LCA comparison analysis confirmed the improvement of energy efficiency and reduction of environmental impact by using the bamboo grid to replace PVC as packing material in cooling towers. The major environmental impact (BEES) indices (e.g., the total Global warming potential, Acidification, Eutrophication and Smog) were reduced by 1.5-10.5 times via the use of bamboo grid. The results demonstrate that bamboo grid packing is a good alternative to replace existing grid packing materials such as concrete and PVC that are harmful to human health and environments.

Association between growth differentiation factor 5 rs143383 genetic polymorphism and the risk of knee osteoarthritis among Caucasian but not Asian: a meta-analysis.

BACKGROUND: A few months ago, the Bioscience Reports journal showed that growth differentiation factor 5 (GDF5) rs143383 genetic polymorphism increases the susceptibility of knee osteoarthritis (KOA), but previous studies' results have debates about available data. Considering the availability of more recent data, we focus on clarifying the relationship of KOA and GDF5 rs143383 genetic polymorphism by a meta-analysis of case-control trial data. METHODS: The eligible studies from the time of database established to Oct. 2019 were collected from PubMed, Springer, Cochrane library, Web of Science, China National Knowledge Infrastructure (CNKI), and Wan Fang library. Odds ratios (OR) and 95% confidence intervals (CI) were used to estimate the association between these polymorphisms and KOA risk. The meta-analysis was completed by STATA 18.0 software. RESULTS: A total of 196 studies were collected, 16 of them included in final meta-analysis (7997 cases and 12,684 controls). There was significant association between GDF5 rs143383 polymorphism and KOA in all genetic models (for Allele model (C versus T): OR = 0.84 (95% CI = 0.76-0.91); dominate model (CC+CT versus TT): OR = 0.80 (95% CI = 0.72-0.90); recessive model (CC versus CT+TT): OR = 0.79 (95% CI = 0.68-0.92); heterozygote model (CT versus CC+TT): OR = 0.89 (95% CI = 0.80-0.97); homozygous model (CC versus TT): OR = 0.71 (95% CI = 0.60-0.85)). In the subgroup analysis, we obtained the results that there is no significance among Asians. CONCLUSION: GDF5 rs143383 genetic polymorphism increases the risk of KOA among Caucasians; CC genotype and C allele are protective factors for the susceptibility of KOA among Caucasians.

Fabricating Pyramidal Lattice Structures of 304 L Stainless Steel by Wire Arc Additive Manufacturing.

Lattice structures have drawn considerable attention due to their superior mechanical properties. However, the existing fabrication methods for lattice structures require complex procedures, as they have low material utilization and lead to unreliable node connections, which greatly restricts their application. In this work, wire arc additive manufacturing is used to fabricate large-scale lattice structures efficiently, without any air holes between rods and panels. The principle and the process of fabricating the rods were analyzed systematically. The influence of the two most important parameters, including heat input and preset layer height, is disclosed. Through optical microscopy, the microstructure of the fabricated steel rods is found to consist of dendritic austenite and skeletal ferrite. The tensile strength of the rods can reach 603 MPa, and their elongation reaches 77%. These experimental results demonstrated the feasibility of fabricating lattice structures using wire arc additive manufacturing.

Lack of association between matrix metalloproteinase-1 gene rs1799750 polymorphism and osteoarthritis susceptibility: a meta-analysis.

Background. A relationship between matrix metalloproteinase-1 (MMP-1)-1607 (rs1799750) gene polymorphism and osteoarthritis (OA) susceptibility was reported in the Bioscience Reports journal; however, these results were inconsistent. To evaluate the specific relationship, we used a meta-analysis study to clarify the controversy. Methods. The relevant articles were retrieved on 20 October 2018 from PubMed, Elsevier, Springer, Ebase (Ovid), and Google Scholar. The number of alleles and genotypes for MMP-1 was obtained. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to estimate the association between MMP-1-1607 (rs1799750) 1G/2G promoter polymorphism and OA, while the Egger's test was used to assess heterogeneity among studies and publication bias. All statistical analyses were conducted using STATA 12.0 software. Results. A total of six case-control studies covering 1133 cases and 1119 controls were included in the final meta-analysis. There was no significant association between MMP-1-1607 1G/2G promoter polymorphism and OA in all genetic models (2G versus 1G: OR = 1.12, 95% CI = 0.78-1.60; 1G/2G versus 1G/1G: OR = 0.73, 95% CI = 0.32-1.67; 2G/2G versus 1G/1G: OR = 1.31, 95% CI = 0.57-2.98; the recessive model: OR = 1.23, 95% CI = 0.63-2.41; and the dominant model: OR = 1.25, 95% CI = 0.79-1.97). We obtained similar results for the subgroup analysis using ethnicity and type of disease. Conclusion. We systematically investigated the association between MMP-1-1607 (rs1799750) 1G/2G polymorphism and OA susceptibility; however, the results show no correlation.

Reducing Porosity and Refining Grains for Arc Additive Manufacturing Aluminum Alloy by Adjusting Arc Pulse Frequency and Current.

Coarse grains and gas pores are two main problems that limit the application of additive manufacturing aluminum alloys. To reduce porosity and refine grains, this paper presents a quantitative investigation into the effect of pulse frequency and arc current on the porosity and grains of arc additive manufacturing Al⁻5Si alloy. The experiment results show that pulse frequency and arc current have a significant impact on the macrostructure, microstructure, porosity, and tensile properties of the samples. Fine grains and a uniform microstructure can be obtained with low pulse frequency and low arc current as a result of the rapid cooling of the molten pool. With the increase of pulse frequency, density shows a trend that firstly escalates and attains the maximum value at 50 Hz, but later declines as a result of the relation between pores formation and gas escape. Moreover, better tensile properties can be obtained at low pulse frequency and low arc current because of the finer grains.

Research on Mechanisms and Controlling Methods of Macro Defects in TC4 Alloy Fabricated by Wire Additive Manufacturing.

Wire feeding additive manufacturing (WFAM) has broad application prospects because of its advantages of low cost and high efficiency. However, with the mode of lateral wire feeding, including wire and laser additive manufacturing, gas tungsten arc additive manufacturing etc., it is easy to generate macro defects on the surface of the components because of the anisotropy of melted wire, which limits the promotion and application of WFAM. In this work, gas tungsten arc additive manufacturing with lateral wire feeding is proposed to investigate the mechanisms of macro defects. The results illustrate that the defect forms mainly include side spatters, collapse, poor flatness, and unmelted wire. It was found that the heat input, layer thickness, tool path, and wire curvature can have an impact on the macro defects. Side spatters are the most serious defects, mainly because the droplets cannot be transferred to the center of the molten pool in the lateral wire feeding mode. This research indicates that the macro defects can be controlled by optimizing the process parameters. Finally, block parts without macro defects were fabricated, which is meaningful for the further application of WFAM.

Effect of Molten Pool Size on Microstructure and Tensile Properties of Wire Arc Additive Manufacturing of Ti-6Al-4V Alloy.

Wire arc additive manufacturing (WAAM) technique is a cost-competitive and efficient technology to produce large structure components in industry domains. Mechanical properties are mainly dominated by the microstructure of the components, which is deeply affected by the molten pool size. In this work, to investigate the effect of the molten pool size on microstructure and mechanical properties of the components, a series of Ti-6Al-4V alloy blocks with different width of molten pool (WMP) ranging from 7 mm to 22 mm were deposited by adjusting the wire feed speed (WFS) from 100 cm/min to 500 cm/min. It is interesting to find that the macrostructure changes from columnar grains to equiaxial grains, and then returns to large columnar grains with the increase of WMP, which is mainly caused by the different cooling rates and thermal gradients. Nonetheless, the tensile properties of the components have a tendency to decline with the increase of WMP.

Wire Arc Additive Manufacturing of AZ31 Magnesium Alloy: Grain Refinement by Adjusting Pulse Frequency.

Wire arc additive manufacturing (WAAM) offers a potential approach to fabricate large-scale magnesium alloy components with low cost and high efficiency, although this topic is yet to be reported in literature. In this study, WAAM is preliminarily applied to fabricate AZ31 magnesium. Fully dense AZ31 magnesium alloy components are successfully obtained. Meanwhile, to refine grains and obtain good mechanical properties, the effects of pulse frequency (1, 2, 5, 10, 100, and 500 Hz) on the macrostructure, microstructure and tensile properties are investigated. The results indicate that pulse frequency can result in the change of weld pool oscillations and cooling rate. This further leads to the change of the grain size, grain shape, as well as the tensile properties. Meanwhile, due to the resonance of the weld pool at 5 Hz and 10 Hz, the samples have poor geometry accuracy but contain finer equiaxed grains (21 µm) and exhibit higher ultimate tensile strength (260 MPa) and yield strength (102 MPa), which are similar to those of the forged AZ31 alloy. Moreover, the elongation of all samples is above 23%.

Performance of High Layer Thickness in Selective Laser Melting of Ti6Al4V.

To increase building rate and save cost, the selective laser melting (SLM) of Ti6Al4V with a high layer thickness (200 µm) and low cost coarse powders (53 µm-106 µm) at a laser power of 400 W is investigated in this preliminary study. A relatively large laser beam with a diameter of 200 µm is utilized to produce a stable melt pool at high layer thickness, and the appropriate scanning track, which has a smooth surface with a shallow contact angle, can be obtained at the scanning speeds from 40 mm/s to 80 mm/s. By adjusting the hatch spacings, the density of multi-layer samples can be up to 99.99%, which is much higher than that achieved in previous studies about high layer thickness selective laser melting. Meanwhile, the building rate can be up to 7.2 mm³/s, which is about 2 times-9 times that of the commercial equipment. Besides, two kinds of defects are observed: the large un-melted defects and the small spherical micropores. The formation of the un-melted defects is mainly attributed to the inappropriate overlap rates and the unstable scanning tracks, which can be eliminated by adjusting the processing parameters. Nevertheless, the micropores cannot be completely eliminated. It is worth noting that the high layer thickness plays a key role on surface roughness rather than tensile properties during the SLM process. Although a sample with a relatively coarse surface is generated, the average values of yield strength, ultimate tensile strength, and elongation are 1050 MPa, 1140 MPa, and 7.03%, respectively, which are not obviously different than those with the thin layer thickness used in previous research; this is due to the similar metallurgical bonding and microstructure.

[Research evolvement of postmortem diagnosis of early myocardial infarction in forensic pathology].

The cardiac sudden death occupies primacy among the cause of death in adult. Myocardial infarction is one of the main mechanisms of sudden death of coronary heart disease. Because of early myocardial infarction happened abruptly and might die in a short time, and the typical or special changes in morphology is absent, so it is the focus of medico legal research forensic fulfill all long. This paper reviewed the characters of myocardial infarction, applications and limits in forensic fields, and prospected feasibility of new clinical markers in forensic practice, and expected to enhance accuracy of postmortem diagnosis in MI.