ABSTRACT
Tobacco (Nicotiana tabacum L.) shows promise for remediating Cd-contaminated soil due to its significant Cd accumulation capabilities. Although various tobacco varieties exhibit distinct Cd bioaccumulation capacities, a comprehensive understanding of the underlying mechanisms is lacking. This study, conducted using hydroponics, explores differences in Cd accumulation and tolerance mechanisms between two tobacco varieties, Basma and Yunyan 87. The results showed that Cd stress reduced the dry weight, tolerance index, and root morphology for both varieties. Basma exhibited a relatively smaller decline in these indices compared to Yunyan 87. Moreover, Basma demonstrated a higher Cd bioconcentration factor (BCF), concentration, and accumulated content, signifying its superior tolerance and bioaccumulation capacity to Cd compared to Yunyan 87. The Carbonyl Cyanide3-ChloroPhenylhydrazone (CCCP) addition resulted in reduced Cd accumulation and BCFs in both tobacco species. This effect was more pronounced in Basma, suggesting that Basma relies more on an active transport process than Yunyan 87. This could potentially explain its enhanced bioaccumulation ability. Subcellular Cd distribution analysis revealed Basma's preference for distributing Cd in soluble fractions, while Yunyan 87 favoured the cell wall fractions. Transmission electron microscope showed that Basma's organelles were less damaged than Yunyan 87's under Cd stress, possibly contributing to the superior tolerance of Basma. Therefore, these results provided a theoretical foundation for development of Cd-contaminated soil tobacco remediation technology.
ABSTRACT
In this study, the mechanism by which mercapto-modified palygorskite (MPAL) mediates Cd and Mn absorption by wheat was elucidated. In the aqueous phase, MPAL can react with Cd to form Cd-thiol complexes and CdO and with Mn to form MnO. In the wheat-soil system, 0.1-0.3% MPAL application increased the biomass of wheat by 18.6-29.4% and decreased the Cd concentration in shoots and roots by 19.4-51.8% and 35.9-64%, respectively; however, MPAL application did not decrease the diethylenetriaminepentaacetic acid (DTPA)-extracted Cd concentration in soil, probably because the formed Cd-thiol complexes and CdO could not be taken up by plants but could be extracted by DTPA. MPAL appeared to increase the Mn concentration in plants and the DTPA-extracted Mn concentration in soil, possibly because of the reduction in soil Mn oxides to more soluble Mn(â ¡) by the thiol groups in MPAL. MPAL enriched plant growth-promoting rhizobacteria and Cd-immobilizing bacteria and strengthened the sulfate reduction metabolism in rhizosphere soil, which partly contributed to the improvement in plant growth and the reduction in Cd bioaccumulation in wheat. These findings highlight the importance of the thiol group in MPAL and the regulation of the rhizosphere bacterial community in mediating Cd and Mn bioaccumulation in wheat.
