ABSTRACT
Amphichorda has been previously accepted as a member of the Cordycipitaceae and currently it is considered a member of the Bionectriaceae. The substrates of Amphichorda were complex and varied, being mainly animal faeces. This study reports two new species of Amphichorda from Yunnan Province in south-western China. Based on the five-gene (nrSSU, nrLSU, tef-1α, rpb1 and rpb2) sequence and ITS data phylogenetic analysis, two new species, namely A.excrementa and A.kunmingensis, are proposed and a detailed description of the new species is provided. Amphichordaexcrementa and A.kunmingensis were isolated from animal faeces in the park. The morphological characteristics of two novel species and seven known species in Amphichorda are also compared.
ABSTRACT
OBJECTIVE: To improve the survival of mesenchymal stem cells (MSCs) for prefabrication of an osteo-musculo-cutaneous flap. METHODS: In a mouse model, the compound of MSCs and p(3HB-co-3HH) were embeded in the latissimus dorsi muscle as an experimental group and the muscle pocket of the buttock as the control. The examinations of the HE staining, hybridization in situ of osteonectin mRNA and Von kossa staining were used to evaluate the results. RESULTS: The expression of osteonectin mRNA and the Von kossa staining showed that the latissimus dorsi muscle group was superior to the control in 2 weeks and 4 weeks after the surgery in vivo. CONCLUSION: The results indicate that the above-mentioned technique may be a good alternative for the prefabrication of the osteo-musculo-cutaneous flap.
Subject(s)
Fascia/transplantation , Surgical Flaps/blood supply , Tissue Engineering/methods , Animals , Histocytochemistry/methods , In Situ Hybridization , Male , Mice , Mice, Nude , Models, Animal , Osteonectin/genetics , Osteonectin/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Staining and Labeling , Stem Cell Transplantation/methodsABSTRACT
OBJECTIVE: To investigate the biocompatibility of p(3HB-co-3HH) and marrow mesenchymal stell cells (MSCs). METHODS: MSCs were inoculated to p(3HB-co-3HH), and then cultured for 2-4 weeks in vitro and embedded for 2 weeks in vivo. The growth, proliferation, morphology and phenotype properties of MSCs were observed by use of phase contrast microscope, electron microscope, HE staining and staining of type I collagen. RESULTS: p(3HB-co-3HH) had good compatibility. The inoculated MSCs could be well-distributed, attached well and obtain the phenotype of MSCs in p(3HB-co-3HH). After osteogenic inducer were added, MSCs differentiated to osteoblasts and secreted matrix. Type I collagen was stained positively by immunohistochemical techenique. CONCLUSION: The above results demonstrate that there is satisfactory biocompatibility between p(3HB-co-3HH) and MSCs.
