1.
Nan Fang Yi Ke Da Xue Xue Bao
; 28(2): 252-4, 2008 Feb.
Article
in Chinese
| MEDLINE
| ID: mdl-18250055
ABSTRACT
OBJECTIVE: To construct the virus-like parcel expressing hepatitis B virus (HBV) C gene and identify its immunogenicity. METHODS: HBV C gene was cloned into the shuttle vector pSC11, and the resulted plasmid pSC11-C was transfected into modified vaccinia virus Ankara (MVA). RESULTS: pSC11-C was correctly constructed as verified by sequence analysis and PCR, and the recombinant virus-like parcel possessed good immunogenicity. CONCLUSION: The MVA-C expressing HBV C gene has been successfully constructed to provide important basis for gene therapy research of chronic HBV infection.
Subject(s)
Genetic Vectors , Hepatitis B Core Antigens/genetics , Vaccinia virus/genetics , Genes, Viral , Recombination, Genetic
2.
Zhonghua Jie He He Hu Xi Za Zhi
; 28(1): 44-6, 2005 Jan.
Article
in Chinese
| MEDLINE
| ID: mdl-15774192