ABSTRACT
OBJECTIVE: To isolate and identify the chemical constituents from Verbena officinalis. METHOD: The compounds were isolated by means of chromatography and the structures were determined on the basis of physical and spectral analysis. RESULT: Four compounds were isolated and identified as apigenin (I), 4'-hydroxywogonin (II), verbenalin (III) and hastatoside (IV). CONCLUSION: Compounds I and II were obtained from the genus for the first time.
Subject(s)
Apigenin/isolation & purification , Flavanones/isolation & purification , Plants, Medicinal/chemistry , Verbena/chemistry , Apigenin/chemistry , Flavanones/chemistry , Iridoid Glycosides , Iridoids/chemistry , Iridoids/isolation & purificationABSTRACT
OBJECTIVE: To establish a RP-HPLC method for determination of glycosides in Traditional Chinese Medicine Liu-wei Di-huang. METHOD: The samples were analyzed on an ODS column at 30 degrees C, with mobile phase of methanol/water (33:67) at flow rate 1.0 mL.min and detection at wavelength of 236 nm. RESULT: Three major components reached base-line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4-60, 7.7-62 mg.L-1 and 8.5-68 mg.L-1, and the recoveries were 98.8%, 98.3%, 99.6%. CONCLUSION: The established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.
Subject(s)
Benzoates/analysis , Bridged-Ring Compounds/analysis , Drugs, Chinese Herbal/analysis , Drugs, Chinese Herbal/chemistry , Glucosides/analysis , Iridoids/analysis , Plants, Medicinal/chemistry , Chromatography, High Pressure Liquid/methods , Cornus/chemistry , Dioscorea/chemistry , Drug Combinations , Monoterpenes , Quality Control , Rehmannia/chemistryABSTRACT
OBJECTIVE: To study the possible mechanism of aqueous extract of detoxified cottonseeds (CTN-W). METHOD AND RESULT: CTN-W 0.01, 0.03, 0.10, 0.30 mg.mL-1 was incubated directly with the synaptic membrane extracted from the cerebral cortex in rats, and adenylyl cyclase (AC) activity was detected by using radio-immunoassay. RESULT: Showed that CTN-W could activate AC in a dose-dependend manner. After incubation with PC12 cells in the presence of corticosterone 2 x 10(-4)mol.L-1 for 48 h, CTN-W 0.08, 0.4, 2 mg.mL-1 protected PC12 cells from the lesion induced by corticosterone. CONCLUSION: Antidepressant and anxiolytic effects of CTN-W are related with the activation of AC-cAMP pathway in signal transduction system, thus protecting neurons from the lesion. These two aspects maybe partly form the mechanism of CTN-W's action.
