[The immunological characteristics and enzymatic activity of lymphocytes from the peripheral blood of the children presenting with pharyngeal tonsillar hypertrophy]. / Immunologicheskie pokazateli i énzimaticheskaia aktivnost' limfotsitov perifericheskoi krovi u detei s gipertrofiei glotochnoi mindaliny.

The objective of the present work was to study the immunological characteristics and activity of NAD(P)-dependent dehydrogenases in peripheral blood lymphocytes in the young children presenting with pharyngeal tonsil hypertrophy (PTH). A total of 57 children at the age from 1 to 3 years with PTH were available for the examination. The control group was comprised of 35 age-matched practically healthy children. The amount of CD3+, CD4+, CD8+, CD16+/56-, and CD19+ cells in the peripheral blood was determined with the use of the cytoflowmetric technique. The activity of NAD(P)-dependent dehydrogenases in the peripheral blood lymphocytes was quantified by the method of A.A. Savchenko and co-workers ([14]. Serum IgA, IgM, and IgG levels were measured as described by G. Mancini and co-workers [12], and the levels of the circulating immune complexes (CIC) by the method of Haskova and co-workers [13]. The children presenting with pharyngeal tonsil hypertrophy were found to undergo changes in the immune-phenotypic spectrum of peripheral bloods lymphocytes, the decrease of serum IgA concentration, and the increase in the serum CIC level. The activity of riboso-5-phpsphate- and NADH-dependent reactions of the macromolecular synthesis was increased whereas the role of the malate-aspartate shunt in the cellular energy metabolism and activity of glycolysis decreased. On the contrary, the substrate flow in the tricarbonic acid cycle was rather high while glutathione reductase activity was low. The present study has shown that the children presenting with pharyngeal tonsil hypertrophy underwent changes in the immune and phenotypic spectrum of peripheral bloods lymphocytes, the decrease of serum IgA concentration, and the increase in the serum CIC level.

[Changes of the Immunophenotypic Spectrum and the Enzymatic Profile of Peripheral Blood Lymphocytes in Infants with Hypertrophy of the Pharyngeal Tonsil].

OBJECTIVE: to study immunophenotype and NAD- and NAD(P)-dependent dehydrogenase of blood lymphocytes activity indicators in children with hypertrophy of the pharyngeal tonsils (HPT). METHODS: 57 children aged 1-3 years with HPT were examined. The focus group included 35 healthy children of the similar age. The number of CD3⁺-, CD4⁺-, CD8⁺-, CD16⁺/56⁺-, CD19⁺-cells in the blood was determined by flow cytometry. The activity of NAD(P)-dependent dehydrogenase was studied by the method of A. Savchenko and coauth. (1989). RESULTS: The changes of immunophenotypic spectrum of peripheral blood lymphocytes in infants with HPT have been revealed. The increase of ribose-5-phosphate and NADN-dependent reactions of macromolecular synthesis, the reduction of malataspartat shunt role in cell energy, the reduction of anaerobic lactate dehydrogenase reaction, the compensatory increase in the glycerol-3-phosphate dehydrogenase activity, the high substrate flow of the citric acid cycle and the reduced level of glutathione have been fixed. The correlation analysis has showed increase in the number of correlations between indicators of investigated oxidoreductase activity in blood lymphocytes in children with HPT and the high level of correlation between the metabolic reactions of the mitochondrial compartment. CONCLUSION: the change of immunophenotype, enzymatic activity, correlation pattern of connection between intracellular enzymes of peripheral blood lymphocytes have been revealed in children aged 1-3 years with HPT.