Catheter-Related Bloodstream Infections in Patients on Emergent Hemodialysis.

OBJECTIVE: This study had 2 objectives: (1) to describe the epidemiology of catheter-related bloodstream infections (CRBSI) in patients with end-stage renal disease (ESRD) who have no access to scheduled dialysis and (2) to evaluate whether a positive culture of the heparin-lock solution is associated with subsequent development of bacteremia. DESIGN: Retrospective observational cohort design for objective 1; and prospective cohort design for objective 2. SETTING AND PARTICIPANTS: The study was conducted in a 770-bed public academic tertiary hospital in Dallas, Texas. The participants were patients with ESRD undergoing scheduled or emergent hemodialysis. METHODS: We reviewed the records of 147 patients who received hemodialysis between January 2011 and May 2011 and evaluated the rate of CRBSI in the previous 5 years. For the prospective study, we cultured the catheter heparin-lock solution in 62 consecutive patients between June 2012 and August 2012 and evaluated the incidence of CRBSI at 6 months. RESULTS: Of the 147 patients on emergent hemodialysis, 125 had a tunneled catheter, with a CRBSI rate of 2.61 per 1,000 catheter days. The predominant organisms were Gram-negative rods (GNR). In the prospective study, we found that the dialysis catheter was colonized more frequently in patients on emergent hemodialysis than in those on scheduled hemodialysis. Colonization with GNR or Staphylococcus aureus was associated with subsequent CRBSI at 6 months follow-up. CONCLUSIONS: Patients undergoing emergent hemodialysis via tunneled catheter are predisposed to Gram-negative CRBSI. Culturing the heparin-lock solution may predict subsequent episodes of CRBSI if it shows colonization with GNR or Staphylococcus aureus. Prevention approaches in this population need to be studied further.

The 2012 West Nile encephalitis epidemic in Dallas, Texas.

IMPORTANCE: After progressive declines over recent years, in 2012 West Nile virus epidemics resurged nationwide, with the greatest number of cases centered in Dallas County, Texas. OBJECTIVE: To analyze the epidemiologic, meteorologic, and geospatial features of the 2012 Dallas West Nile virus epidemic to guide future prevention efforts. DESIGN, SETTING, AND PATIENTS: Public health surveillance of Dallas County, an area of 2257 km2 and population of 2.4 million. Surveillance data included numbers of residents diagnosed with West Nile virus infection between May 30, 2012, and December 3, 2012; mosquito trap results; weather data; and syndromic surveillance from area emergency departments. MAIN OUTCOMES AND MEASURES: Incidence and age-adjusted incidence rates of West Nile neuroinvasive disease (WNND), daily prevalence of emergency department visits for asthma and skin rash, and Culex quinquefasciatus species-specific vector index (an estimate of the average number of West Nile virus-infected mosquitoes per trap-night). RESULTS: The investigation identified 173 cases of WNND, 225 of West Nile fever, 17 West Nile virus-positive blood donors, and 19 deaths in 2012. The incidence rate for WNND was 7.30 per 100,000 residents in 2012, compared with 2.91 per 100,000 in 2006, the largest previous Dallas County outbreak. An unusually rapid and early escalation of large numbers of human cases closely followed increasing infection trends in mosquitoes. The Cx quinquefasciatus species-specific vector index predicted the onset of symptoms among WNND cases 1 to 2 weeks later (count regression ß = 2.97 [95% CI, 2.34 to 3.60]; P < .001). Although initially widely distributed, WNND cases soon clustered in neighborhoods with high housing density in the north central area of the county, reflecting higher vector indices and following geospatial patterns of West Nile virus in prior years. During the 11 years since West Nile virus was first identified in Dallas, the log-transformed annual prevalence of WNND was inversely associated with the number of days with low temperatures below 28°F (-2.2°C) in December through February (ß = -0.29 [95% CI, -0.36 to -0.21]; P < .001). Aerial insecticide spraying was not associated with increases in emergency department visits for respiratory symptoms (ß = -4.03 [95% CI, -13.76 to 5.70]; P = .42) or skin rash (ß = -1.00 [95% CI, -6.92 to 4.92]; P = .74). CONCLUSIONS AND RELEVANCE: Large West Nile virus epidemics in Dallas County begin early after unusually warm winters, revisit similar geographical distributions, and are strongly predicted by the mosquito vector index. Consideration of weather patterns and historical geographical hot spots and acting on the vector index may help prevent West Nile virus-associated illness.

Presentation of seasonal influenza A in pregnancy: 2003-2004 influenza season.

OBJECTIVE: To describe the clinical course of influenza in pregnant women followed at our institution during the 2003-2004 influenza season. METHODS: This was a prospective evaluation of all pregnant women diagnosed with influenza A between October 22, 2003, and January 18, 2004. Pregnant women presenting with a flu-like illness were evaluated using a rapid diagnostic test, culture, or both. Patients were admitted in accordance with prospectively developed clinical protocols. Women with a laboratory-confirmed diagnosis were treated with Centers for Disease Control-recommended antivirals. RESULTS: Influenza A was confirmed in 107 patients. All viral isolates obtained were of the H3N2 strain. Influenza was most commonly diagnosed in the third trimester (45%). Cough was the most commonly reported symptom (93%), followed by myalgias (61%), nausea or vomiting (60%), and rhinorrhea (56%). Eighty-four percent of the women had no comorbid conditions; however, 62% required admission. Twenty-one percent of patients had a maximum heart rate higher than 130 beats per minute. Complications of influenza A included pneumonia (12%), meningitis (1%), and myocarditis (1%). There were no maternal deaths. Eighty-one (76%) of the women delivered at our institution. When compared with our general obstetric population, there was no significant difference in obstetric or neonatal complications. CONCLUSION: Influenza A in pregnancy is characterized by cough, myalgia, nausea or vomiting, and rhinorrhea. Profound tachycardia disproportionate to maternal fever uniquely affected the majority of the women in our cohort. Pneumonia complicated one in eight cases; however, the anticipated excess maternal morbidities and mortality did not occur.

Detection and diagnosis of herpes simplex virus infection in adults with acute liver failure.

Disseminated herpes simplex virus (HSV) infection may lead to acute liver failure (ALF) and the need for emergency liver transplantation (LT). The primary aim of this study was to determine the utility of HSV serological testing and HSV DNA testing by polymerase chain reaction (PCR) in the diagnosis and management of indeterminate, pregnancy-related, and known HSV-related ALF. Stored sera obtained on study day 1 or 2 from patients enrolled in the United States ALF Study Group with indeterminate (n = 51), pregnancy-related (n = 12), and HSV-related (n = 4) ALF were screened for HSV DNA by PCR and serology. While 7 of the indeterminate and pregnant patients had positive anti-HSV immunoglobulin M, none had detectable HSV DNA. The 4 known HSV cases all had high-titer HSV DNA on presentation (range: 3.5 to 36 x 10(8) copies/mL). Two HSV patients underwent LT but developed posttransplant extrahepatic HSV infection despite suppression of HSV DNA with acyclovir treatment, and one of them eventually died. The 2 other fulminant HSV patients died within 48 hours of presentation. In conclusion, serum HSV DNA indicative of occult HSV infection was not detected in 51 indeterminate and 12 pregnancy-related ALF patients. The 4 patients with known HSV-related ALF all had high HSV DNA levels at presentation, and despite the rapid use of antiviral therapy and emergency LT, substantial morbidity and mortality were encountered, highlighting the poor prognosis with severe disseminated HSV infection.

Pandemic influenza: a primer.

Influenza maintains a special position in human medicine as the cause of the greatest pandemic of disease in all of human history as well as a continuous and significant source of worldwide morbidity and mortality every year. The very nature of the influenza virus allows it to evade and adapt to the human population. We currently have useful tools such as vaccination and antiviral medications to limit this burden; however, appropriate vaccines take time to prepare, and antiviral drug resistance has emerged as a significant problem. Continued vigilance and pandemic planning are essential, given the continued threat of novel strains that can arise and rapidly cause disease across the world.

Recurrent exposure to Histoplasma capsulatum in modern air-conditioned buildings.

BACKGROUND: Between 1989 and 1996, an epidemic of histoplasmosis occurred on a medical school campus. There had been numerous construction projects on the campus that involved previously wooded land and were adjacent to a large bird sanctuary. METHODS: We investigated the epidemic with active surveillance to detect cases, a histoplasmin skin-test survey, inspection of the air-filtration systems of the involved buildings, and cultures of soil samples. The investigation also included a simulation of entry into air-intakes of the buildings from spore sources by means of a wind-tunnel analysis of a model of the campus that used inert gas. After control procedures were instituted, sentinel population groups had follow-up with yearly serological tests. RESULTS: From 1989 through 1996, there were 29 cases of histoplasmosis that occurred among school employees. All cases with a defined onset began during periods of ongoing campus construction. Positivity rates for histoplasmin skin testing were higher among on-campus personnel (47%) than among off-campus employee control subjects (28%) (P<.001); the rates were highest in employees who worked on the upper floors of 2 research buildings. The air-handling units on the roofs of these buildings were not designed to exclude Histoplasma spores. The wind-tunnel experiment indicated that spores aerosolized in the bird sanctuary were not taken into campus buildings. CONCLUSIONS: The major sources of employee exposure to H. capsulatum spores were the construction sites. Low-level, recurrent exposures occurred over several years inside modern research buildings. This phenomenon, which has not been previously described, may play a role in the epidemiology of spore-transmitted diseases in urban settings.

Fatal type 3 adenoviral pneumonia in immunocompetent adult identical twins.

We describe adult twin sisters who developed severe adenoviral pneumonia with relative leukopenia, progressive focal infiltrates, shock, and hypoxia. Potential determinants of severe adenoviral disease are discussed.