ABSTRACT
OBJECTIVE: HPV-DNA integration is one factor for malignant transformation and can be identified using in situ hybridization (ISH), where a diffuse signal represents episomal HPV and punctate, integrated. The aim is to verify if a punctate pattern could be a marker of CIN1 that progresses. METHODS: 74 CIN1 biopsies were studied. In the follow up, a second biopsy was performed and 65% showed CIN1 or no lesion (group without progression) and 35% CIN2/3 (with progression). ISH was carried out with HR-HPV GenPoint in the first biopsy looking for the positive distribution in epithelium regions (basal, intermediate, superficial) and reaction pattern (diffuse and punctate). The Mann-Whitney and Fisher tests were used to compare the groups (pSubject(s)
Papillomaviridae/genetics
, Papillomavirus Infections/complications
, Papillomavirus Infections/virology
, Uterine Cervical Dysplasia/pathology
, Uterine Cervical Dysplasia/virology
, Uterine Cervical Neoplasms/pathology
, Uterine Cervical Neoplasms/virology
, Adolescent
, Adult
, Aged
, Biopsy
, Cell Transformation, Viral
, DNA, Viral/genetics
, Disease Progression
, Female
, Humans
, In Situ Hybridization
, Middle Aged
, Retrospective Studies
, Virus Integration
, Young Adult