ABSTRACT
To determine the role of biomarkers in the clinical management of respiratory complications (RC) in hematopoietic stem cell transplantation (HSCT) recipients, we have prospectively evaluated a cohort of 175 patients followed-up for 1 year after HSCT. To avoid misinterpretation, we have excluded both unidentified respiratory infections (RI) and mixed RI. A total of 64 RC were included. Plasma levels of C-reactive protein (CRP), procalcitonin (PCT) and proadrenomedullin (proADM) were measured at diagnosis and on day 3 and 7. Different cytokines were evaluated in serum on the first day. No HSCT recipients without RC were included as a control group. Compared with RI, non-infectious RC showed a significant increase in CRP, proADM and interleukin 6 on day 0 (P=0.005; P=0.03 and P=0.04, respectively). When only RI were considered, we observed that bacterial-fungal PI showed higher levels of CRP (P=0.02), PCT (P=0.04) and proADM (P<0.01). Persistent low levels of proADM biomarkers suggest viral infection (specificity and positive predictive value 100%). Patients dying of RC had PCT and proADM levels higher than survivors (P=0.002 and P=0.03, respectively). In HSCT recipients biomarkers increase in both infectious and non-infectious RC. They may have utility in the assessment of the severity of RC and in suspecting a viral etiology.
Subject(s)
Bacterial Infections , C-Reactive Protein/metabolism , Hematopoietic Stem Cell Transplantation , Interleukin-6/blood , Mycoses , Respiratory Tract Infections , Adult , Allografts , Bacterial Infections/blood , Bacterial Infections/etiology , Bacterial Infections/mortality , Biomarkers/blood , Disease-Free Survival , Female , Follow-Up Studies , Hematologic Neoplasms/blood , Hematologic Neoplasms/mortality , Hematologic Neoplasms/therapy , Humans , Male , Middle Aged , Mycoses/blood , Mycoses/etiology , Mycoses/mortality , Respiratory Tract Infections/blood , Respiratory Tract Infections/etiology , Respiratory Tract Infections/mortality , Survival RateABSTRACT
RESUMO No Nordeste do Brasil é bastante comum e variado o uso da coroa-de-frade (Melocactus spp.). Assim, o objetivo deste estudo foi realizar diversas análises em cladódios de coroa-de-frade [Melocactus zehntneri (Britton & Rose) Luetzelburg], para que possamos compreender as razões estes usos. Os cladódios foram coletados na cidade de Campina Grande e levados ao Laboratório de Engenharia de Alimentos da Universidade Federal de Campina Grande, onde foram feitas as seguintes análises: peso, altura, diâmetro, cor, umidade, acidez, pH, ácido ascórbico, carotenoides, flavonoides e compostos fenólicos, tanto na epiderme quanto no parênquima aquífero. Os resultados mostraram que a epiderme tem uma acidez elevada e mais ácido ascórbico, e um pH mais baixo em comparação com o parênquima aquífero. O conteúdo de carotenoides, flavonoides e compostos fenólicos, foram diferentes nas partes estudadas. A epiderme apresentou sempre maiores quantidades de carotenoides, flavonoides e compostos fenólicos em relação ao parênquima aquífero, nas três plantas estudadas, indicando que a maior parte da defesa da planta acontece neste tecido. Mais estudos são necessários com técnicas mais precisas, para identificar os componentes bioativos desta espécie.
ABSTRACT In Northeast Brazil, the use of melon cactus (Melocactus spp.) is quite common and varied. Thus, the present study aimed to perform various analyzes in cladodes of melon cactus [Melocactus zehntneri (Britton & Rose) Luetzelburg], to understand the reasons for the use of this species. The cladodes were collected in the city of Campina Grande and taken to the Food Engineering Laboratory of the Federal University of Campina Grande, where we performed the following analyses: mass, height, diameter, color, moisture, acidity, pH, ascorbic acid, carotenoids, flavonoids and phenolic compounds, both in the epidermis and in the aquifer parenchyma. The results showed that the epidermis has a higher acidity, ascorbic acid content and lower pH compared to the aquifer parenchyma. The contents of carotenoids, flavonoids and phenolic compounds, were different from the both parts analyzed. The epidermis always had greater quantities of carotenoids, flavonoids and phenolic compounds than the aquifer parenchyma for the three plants, showing that most of the plant defense takes place in this tissue. Further studies, with more accurate techniques, are needed to identify the bioactive components of this species.
Subject(s)
Cactaceae/classification , Ascorbic Acid/analysis , Carotenoids/analysis , Plant Stems/classification , Phenolic Compounds/analysisABSTRACT
Pulmonary complications are common and often lethal in hematopoietic SCT recipients. The objective of this prospective interventional study was to evaluate the etiology, diagnostic procedures, risk factors and outcome of pulmonary complications in a cohort of hematopoietic SCT recipients followed up for 1 year. For patients suffering from a pulmonary complication, a diagnostic algorithm that included non-invasive and bronchoscopic procedures was performed. We identified 73 pulmonary complications in 169 patients: 50 (68%) were pneumonias; 21 (29%) were non-infectious complications and 2 (3%) were undiagnosed. Viruses (particularly Rhinovirus) and bacteria (particularly P. aeruginosa) (28 and 26%, respectively) were the most common causes of pneumonia. A specific diagnosis was obtained in 83% of the cases. A non-invasive test gave a specific diagnosis in 59% of the episodes. The diagnostic yield of bronchoscopy was 67 and 78% in pulmonary infections. Early bronchoscopy (⩽5 days) had higher diagnostic yield than late bronchoscopy (78 vs 23%; P=0.02) for pulmonary infections. Overall mortality was 22 and 32% of all fatalities were due to pulmonary complications. Pulmonary complications are common and constitute an independent risk factor for mortality, stressing the importance of an appropriate clinical management.
Subject(s)
Hematopoietic Stem Cell Transplantation/adverse effects , Lung Diseases/etiology , Transplantation Conditioning/adverse effects , Cohort Studies , Female , Humans , Male , Middle Aged , Prospective StudiesABSTRACT
Objetivo: Describir las indicaciones, rentabilidad diagnóstica y complicaciones de la fibrobroncoscopia (FBS) en una unidad de vigilancia intensiva respiratoria (UVIR). Diseño: Estudio prospectivo observacional. Ámbito: UVIR de 6 camas en un hospital universitario de tercer nivel. Pacientes: Pacientes admitidos en una UVIR a los que se les realizó una FBS. Intervenciones: Ninguna. Variables de interés: Indicaciones y complicaciones de la FBS, técnicas endoscópicas realizadas y tiempo empleado en la FBS. Resultados: Se realizaron 107 (23%) FBS a 69 de los 297 pacientes admitidos en la UVIR. El 68% de las FBS se practicaron a pacientes con ventilación mecánica. La FBS se realizó con fines diagnósticos en 88 ocasiones (82%) y terapéuticos en 19 (18%). La indicación más frecuente para la FBS diagnóstica fue el estudio de infiltrados pulmonares (44 casos; 50%), particularmente en pacientes inmunodeprimidos (24 casos; 27%). Para esta indicación, la rentabilidad diagnóstica de la FBS fue significativamente mejor en los pacientes inmunodeprimidos, respecto a los inmunocompetentes (48% vs 30%; p<0,01). La FBS no causó complicaciones mayores; únicamente se observó un descenso significativo en la PaO2/FiO2 (182±74 vs 163±79; p<0,005) cuando se realizó un lavado broncoalveolar. La mortalidad global en la UVIR fue del 14%; del 25% en los pacientes que precisaron FBS y del 45% en aquellos que precisaron FBS adicionales. Conclusiones: La FBS es un procedimiento seguro y rápido que se utiliza con frecuencia en la UVIR y que contribuye significativamente al manejo clínico. Los pacientes de la UVIR que requieren FBS adicionales tienen una elevada mortalidad (AU)
Objective: To describe the indications, diagnostic performance and safety of fiberoptic bronchoscopy (FOB) performed in a respiratory intensive care unit (RICU). Design: A prospective, observational study was carried out. Setting: A 6-bed RICU in a tertiary university hospital. Patients: Patients admitted to RICU who required FOB. Interventions: None. Main measurements: FOB indications and complications, endoscopic procedures, time required to perform FOB. Results: Sixty-nine out (23%) of the 297 patients admitted to the RICU underwent a total of 107 FOB. Sixty-eight percent of FOB were performed in patients on mechanical ventilation. FOB was performed for diagnostic and therapeutic purposes in 88 (82%) and 19 cases (18%), respectively. The study of pulmonary infiltrates was the main indication for diagnostic FOB (44 cases; 50%), particularly in immunocompromised patients (24 cases; 27%). In immunocompromised patients the diagnostic performance of FOB was significantly higher than in immunocompetent subjects (48% vs 30%; p<0.01). No major complications were recorded. Only a significant drop in PaO2/FiO2 ratio was observed (182±74 vs 163±79; p<0.005) in patients undergoing bronchoalveolar lavage. Overall mortality in patients in the RICU was 14%. In patients requiring a single FOB procedure, mortality was 25%, versus 45% among those requiring more than one FOB procedure. Conclusions: These results show that FOB is used commonly in the RICU. It is a safe and fast procedure that contributes significantly to clinical management. Patients requiring additional FOB during admission to the RICU show high mortality (AU)
Subject(s)
Humans , Bronchoscopy/methods , Respiratory Tract Diseases/diagnosis , Respiration, Artificial/adverse effects , Intensive Care Units , Prospective Studies , Bronchoalveolar Lavage , Immunocompromised HostABSTRACT
OBJECTIVE: To describe the indications, diagnostic performance and safety of fiberoptic bronchoscopy (FOB) performed in a respiratory intensive care unit (RICU). DESIGN: A prospective, observational study was carried out. SETTING: A 6-bed RICU in a tertiary university hospital. PATIENTS: Patients admitted to RICU who required FOB. INTERVENTIONS: None. MAIN MEASUREMENTS: FOB indications and complications, endoscopic procedures, time required to perform FOB. RESULTS: Sixty-nine out (23%) of the 297 patients admitted to the RICU underwent a total of 107 FOB. Sixty-eight percent of FOB were performed in patients on mechanical ventilation. FOB was performed for diagnostic and therapeutic purposes in 88 (82%) and 19 cases (18%), respectively. The study of pulmonary infiltrates was the main indication for diagnostic FOB (44 cases; 50%), particularly in immunocompromised patients (24 cases; 27%). In immunocompromised patients the diagnostic performance of FOB was significantly higher than in immunocompetent subjects (48% vs 30%; p<0.01). No major complications were recorded. Only a significant drop in PaO(2)/FiO(2) ratio was observed (182 ± 74 vs 163 ± 79; p<0.005) in patients undergoing bronchoalveolar lavage. Overall mortality in patients in the RICU was 14%. In patients requiring a single FOB procedure, mortality was 25%, versus 45% among those requiring more than one FOB procedure. CONCLUSIONS: These results show that FOB is used commonly in the RICU. It is a safe and fast procedure that contributes significantly to clinical management. Patients requiring additional FOB during admission to the RICU show high mortality.
