ABSTRACT
A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
Subject(s)
Cucurbita/virology , Genome, Viral , Phylogeny , Potyvirus/genetics , RNA, Viral/genetics , Argentina , Base Sequence , High-Throughput Nucleotide Sequencing , Open Reading Frames , Plant Diseases/virology , Potyvirus/classification , Potyvirus/isolation & purification , Sequence Analysis, DNA , Sequence Homology, Nucleic AcidABSTRACT
The findings of Amblyomma tigrinum in continental Argentina north of 40 degrees S are presented according to phytogeographic domains (Andean-Patagonic, Amazonian and Chaco) and the seasonal distribution is depicted on a monthly basis. A total of 718 adult ticks and four nymphs were found. A. tigrinum ticks were present in all domains. Adult ticks were found all year round but they were more abundant during the summer. Most ticks were found on domestic dogs but the survey has a bias to domestic hosts. It is speculated that the cycle of immature is bound to shelters that mitigate extreme climatic conditions, thus explaining the finding of this tick species in contrasting ecological areas.
