1.
J Microsc
; 242(3): 221-7, 2011 Jun.
Article
in English
| MEDLINE
| ID: mdl-21175615
ABSTRACT
Cryo-electron tomography allows three-dimensional visualization of frozen-hydrated, vitrified biological material at molecular resolution. Here, we summarize the most important sample preparation methods and technical aspects relevant for cryo-electron tomography, as well as its recent biological applications from isolated macromolecular complexes to entire cells and tissues.