ABSTRACT
BACKGROUND AND PURPOSE: Centrally acting histamine H(3) receptor ligands are proposed as potential treatments for obesity, although the value of inverse agonists at these receptors is still debated. Functional inhibition of H(3) autoreceptors activates neurones in a hypothalamic 'satiety' centre. The H(3) receptor antagonist, proxyfan was used as a tool to assess the action of histaminergic compounds in this model. EXPERIMENTAL APPROACH: We compared the actions of histamine on feeding with those of an H(3) receptor agonist (imetit) and inverse agonist (thioperamide) in rats and mice. Sites of action were identified by immunohistochemistry and the hypothalamic ventromedial nucleus (VMN) was investigated using electrophysiological techniques. KEY RESULTS: Central histamine or thioperamide decreased fast-induced feeding, whereas imetit increased feeding. Systemic thioperamide entered the brain to activate hypothalamic feeding centres and to reduce feeding without causing any adverse behaviours. Thioperamide activated neurones in the VMN through an action on histamine autoreceptors, whilst imetit had the opposite effect. Proxyfan administered alone did not affect either feeding or electrical activity. However, it blocked the actions of both thioperamide and imetit, acting as a neutral antagonist in this system. CONCLUSIONS AND IMPLICATIONS: The H(3) receptor inverse agonist, thioperamide, potently reduced appetite without adverse behavioural effects. This action was blocked by proxyfan, acting as a neutral antagonist in this model and, therefore, this compound is useful in determining the selectivity of H(3) receptor-directed drugs. A major action of thioperamide is through presynaptic autoreceptors, inducing stimulation by endogenous histamine of postsynaptic H(1 ) receptors on anorectic hypothalamic neurones.
Subject(s)
Eating/drug effects , Histamine Antagonists/pharmacology , Imidazoles/pharmacology , Receptors, Histamine H3/physiology , Ventromedial Hypothalamic Nucleus/physiology , Animals , Eating/physiology , Histamine/pharmacology , Histamine Agonists/pharmacology , Male , Mice , Piperidines/pharmacology , Rats , Rats, Sprague-Dawley , Thiourea/analogs & derivatives , Thiourea/pharmacologyABSTRACT
Endocannabinoids have a variety of effects by acting through cannabinoid 1 (CB1) receptors located throughout the brain. However, since CB1 receptors are located presynaptically, and because the strength of downstream coupling varies with brain region, expression studies alone do not provide a firm basis for interpreting sites of action. Likewise, to date most functional studies have used high doses of drugs, which can bias results toward non-relevant adverse effects, and which mask more behaviourally-relevant actions. Here we use a low, orexigenic dose of the full CB1 agonist, CP55940, to map responsive brain regions using the complementary techniques of pharmacological-challenge functional magnetic resonance imaging (phMRI) and immediate-early gene activity. Areas of interest demonstrate a drug interaction when the CB1 receptor inverse agonist, rimonabant, is co-administered. This analysis highlights the corticostriatal-hypothalamic pathway, which is central to the motivational drive to eat.
Subject(s)
Brain/physiology , Cannabinoids/metabolism , Eating/physiology , Receptor, Cannabinoid, CB1/metabolism , Animals , Brain/drug effects , Brain Mapping , Central Nervous System Agents/pharmacology , Cyclohexanols/pharmacology , Drug Interactions , Genes, Immediate-Early/physiology , Hyperphagia/chemically induced , Hyperphagia/drug therapy , Hyperphagia/metabolism , Immunohistochemistry , Magnetic Resonance Imaging , Male , Oxygen/blood , Piperidines/pharmacology , Proto-Oncogene Proteins c-fos/metabolism , Pyrazoles/pharmacology , Rats , Rats, Sprague-Dawley , Receptor, Cannabinoid, CB1/agonists , RimonabantABSTRACT
Potassium chloride ion cotransporters (KCCs) are part of a family of transporters classically described as being involved in cell volume regulation. Recently, KCC2 has been shown to have a role in the development of the inhibitory actions of amine transmitters, whereas KCC3 also plays a fundamental role in the development and function of the central and peripheral nervous system. We have re-assessed the expression of each of the known KCCs in the rat forebrain using RT-PCR and in situ hybridisation histochemistry. As well as confirming the widespread expression of KCC1 and KCC2 throughout the brain, we now show a more restricted expression of KCC3a in the hippocampus, choroid plexus and piriform cortex, as well as KCC4 in the choroid plexus and the suprachiasmatic nucleus of the hypothalamus. The expression of KCC4 in the latter and KCC2 in the lateral hypothalamic and ventromedial hypothalamic nuclei suggests that these cotransporters may have selective roles in neuroendocrine or homeostatic functions. Finally, we demonstrate the existence of a truncated splice variation of KCC3a in the rat that appears to be expressed exclusively in neurons (as is KCC2), whereas the native form of KCC3a and KCC4 appears to be expressed in glial cells.
Subject(s)
Gene Expression/physiology , Prosencephalon/metabolism , Symporters/metabolism , Animals , Animals, Newborn , Blotting, Northern/methods , Cells, Cultured , Coculture Techniques/methods , Embryo, Mammalian , Immunohistochemistry/methods , In Situ Hybridization/methods , Male , Mice , Mice, Inbred C57BL , Neuroglia/chemistry , Neuroglia/metabolism , Neurons/metabolism , Phosphopyruvate Hydratase/metabolism , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Reverse Transcriptase Polymerase Chain Reaction/methods , Symporters/geneticsABSTRACT
Systemic injection of peptide YY3-36 reduces food intake in rodents and humans, although some groups have reported a lack of response. PYY3-36 is thought to act via the Y2 receptor to presynaptically inhibit the release of neuropeptide Y and GABA from hypothalamic arcuate neurones. Due to the controversy surrounding its action in rodents, we tested the peptide intravenously on feeding behaviour in rats and attempted to block its actions with the Y2 receptor antagonist BIIE0246. PYY3-36 significantly decreased food intake during the first hour in male Sprague-Dawley rats fasted overnight and then re-fed. BIIE0246 had no effect alone on re-feeding, but completely blocked the action of PYY3-36. In a second experiment of similar design, the behavioural satiety sequence (BSS) was studied. Normal rats eat, drink, explore and groom before entering rest. PYY3-36 significantly reduced food eaten maintaining the normal BSS, although shifting it to the left as expected for a natural satiety factor. The latency to rest occurred earlier for animals given PYY3-36 alone and PYY3-36 tended to increase the total time in rest compared with controls. These behavioural effects of PYY3-36 were blocked by BIIE0246, and BIIE0246 alone did not have an effect on the BSS. These results support the role of PYY3-36 as a natural satiety factor acting through Y2 receptors.
Subject(s)
Arginine/analogs & derivatives , Feeding Behavior/drug effects , Feeding Behavior/physiology , Peptide YY/pharmacology , Receptors, Neuropeptide Y/antagonists & inhibitors , Animals , Arginine/pharmacology , Benzazepines/pharmacology , Eating/drug effects , Eating/physiology , Fasting , Injections, Intravenous , Male , Peptide Fragments , Rats , Rats, Sprague-Dawley , Satiety Response/drug effects , Satiety Response/physiologyABSTRACT
Signal transducers and activators of transcription (STATs) are a family of transcription factors linked to class I cytokine receptors. In the present study, we investigated whether their distribution in the hypothalamus reflects the feedback regulation by growth hormone and what role they might play in the functioning of target neurones. We demonstrate that each of the seven known STATs has a distinct distribution in the hypothalamus. Notably, the STAT5 proteins, that are important in growth hormone (GH) and prolactin signalling in peripheral tissues, were expressed in somatostatin neurones of the periventricular nucleus and dopamine neurones of the arcuate nucleus. Because somatostatin neurones are regulated by feedback from circulating GH, we investigated the importance of STAT5 in these neurones. We demonstrate that STAT5b protein expression, similar to somatostatin mRNA, is sexually dimorphic in the periventricular nucleus of rats and mice. Furthermore, chronic infusion of male dwarf rats with GH increased the expression of STAT5b, while a single injection of GH into similar rats induced the phosphorylation of STAT5 proteins. The cellular abundance of somatostatin mRNA in STAT5b-deficient mice was significantly reduced in the periventricular nucleus, effectively reducing the sexually dimorphic expression. These results are consistent with the hypothesis that STAT5 proteins are involved in the feedback regulation of somatostatin neurones by GH, and that these neurones may respond to patterned GH secretion to reinforce sexual dimorphism in the GH axis.
Subject(s)
DNA-Binding Proteins/physiology , Growth Hormone/physiology , Hypothalamus/metabolism , Neurons/metabolism , Somatostatin/metabolism , Trans-Activators/physiology , Animals , DNA-Binding Proteins/deficiency , DNA-Binding Proteins/genetics , Disease Models, Animal , Dwarfism, Pituitary/metabolism , Feedback, Physiological/physiology , Female , Growth Hormone/deficiency , Hypothalamus/cytology , Male , Mice , Mice, Knockout , Midline Thalamic Nuclei/cytology , Midline Thalamic Nuclei/metabolism , Milk Proteins/genetics , Rats , Rats, Mutant Strains , STAT5 Transcription Factor , Sex Characteristics , Signal Transduction/genetics , Signal Transduction/physiology , Trans-Activators/deficiency , Trans-Activators/geneticsABSTRACT
Centrally administered neuromedin U (NMU) has profound effects on food intake and energy expenditure. In the rat, central expression of NMU mRNA is confined to the brainstem and the hypothalamus/pituitary, while mRNA for the receptor NMU2R is expressed in the hypothalamus and hippocampus, as well as in the lining of the ventricular system, but not in the brainstem. We demonstrate that a subpopulation of catecholaminergic neurones in the brainstem nucleus of the tractus solitarius contain NMU and are activated by the gut-derived peptide, cholecystokinin. This is consistent with NMU neurones having an anorectic action, probably via their interaction with other neurones in the paraventricular hypothalamus.
Subject(s)
Cholecystokinin/physiology , Neurons/metabolism , Neuropeptides/metabolism , Receptors, Adrenergic, alpha-2/metabolism , Solitary Nucleus/metabolism , Animals , Brain Stem/cytology , Brain Stem/metabolism , Catecholamines/metabolism , Feeding Behavior/physiology , In Situ Hybridization , Male , Nerve Net/physiology , Rats , Satiety Response/physiology , Solitary Nucleus/cytology , Tissue DistributionABSTRACT
Galanin-like peptide (GALP) is a recently identified neuropeptide that shares sequence homology with the orexigenic neuropeptide, galanin. In contrast to galanin, GALP is reported to bind preferentially to the galanin receptor 2 subtype (GalR2) compared to GalR1. The aim of this study was to determine the effect of GALP on feeding, body weight and core body temperature after central administration in rats compared to the effects of galanin. Intracerebroventricular (i.c.v.) injection of GALP (1 micro g-10 micro g) significantly stimulated feeding at 1 h in both satiated and fasted Sprague-Dawley rats. However, 24 h after GALP injection, body weight gain was significantly reduced and food intake was also usually decreased. In addition, i.c.v. GALP caused a dose-related increase in core body temperature, which lasted until 6-8 h after injection, and was reduced by peripheral administration of the cyclooxygenase inhibitor, flurbiprofen (1 mg/kg). Similar to GALP, i.c.v. injection of galanin (5 micro g) significantly increased feeding at 1 h in satiated rats. However, there was no difference in food intake and body weight at 24 h, and galanin only caused a transient rise in body temperature. Thus, similar to galanin, GALP has an acute orexigenic effect on feeding. However, GALP also has an anorectic action, which is apparent at a later time. Therefore, GALP has complex opposing actions on energy homeostasis.
Subject(s)
Eating/drug effects , Nerve Tissue Proteins/administration & dosage , Animals , Cyclooxygenase Inhibitors/pharmacology , Dose-Response Relationship, Drug , Fasting/physiology , Flurbiprofen/pharmacology , Galanin/administration & dosage , Galanin/pharmacology , Galanin-Like Peptide , Injections, Intraventricular , Male , Nerve Tissue Proteins/pharmacology , Rats , Rats, Sprague-Dawley , Satiation/physiologyABSTRACT
Prolactin (PRL) has been implicated in central actions including those that result in its own regulation and/or the suppression of gonadotropin secretion. It is not clear, however, which neuronal systems may mediate the central effects of PRL. Here, using dual immunohistochemistry for c-Fos and either tyrosine hydroxylase (TH) or proopiomelanocortin (POMC), we have assessed neuronal activation, following centrally administered PRL, within two neuronal networks that have been shown to participate in the inhibitory regulation of reproductive function. Male rats received one intracerebroventricular injection of either PRL (5 microg) or saline (vehicle control) 5 days after cannulae were inserted into the lateral ventricles. Ninety minutes after treatment, animals were perfused with 4% paraformaldehyde, the brains were removed and 30-microm frozen sections were cut throughout the entire hypothalamic region. Parallel sets of sections were processed for both c-Fos immunoreactivity (ir) and either TH-ir or POMC-ir. PRL increased the mean number of c-Fos-ir neurons within the rostral arcuate nucleus (9.3 +/- 2.0 vs. 5.0 +/- 1.2 cells/section, for PRL and control rats, respectively; p < 0.05). Within the TH-ir neurones, PRL induced a significant increase in c-Fos in the dorsomedial portion of the mid-arcuate nucleus (p < 0.05). In contrast, there was no significant increase in the expression of c-Fos within the POMC neurones of the arcuate nucleus. PRL also induced c-Fos expression in the supraoptic nucleus (SON) (11.7 +/- 3.2 vs. 3.0 +/- 1.4 cells/section for PRL and control rats, respectively; p < 0.05), but not in the medial preoptic nucleus, ventromedial nucleus or the dorsomedial nucleus, areas reported to either contain gonadotropin-releasing hormone neurones or express PRL receptors. The results from this study show immediate early gene activation within both the arcuate nucleus and the SON of the hypothalamus following acute PRL administration. While the role of PRL-responsive neurones in the SON remains to be elucidated, these findings support the notion that the central actions of PRL could be mediated via the TH neurones of the dorsomedial arcuate nucleus and/or by a population of neurones in the rostral arcuate nucleus that contain neither TH nor POMC.
Subject(s)
Arcuate Nucleus of Hypothalamus/metabolism , Neurons/metabolism , Pro-Opiomelanocortin/metabolism , Prolactin/pharmacology , Proto-Oncogene Proteins c-fos/metabolism , Tyrosine 3-Monooxygenase/metabolism , Animals , Arcuate Nucleus of Hypothalamus/cytology , Hypothalamus/physiology , Immunohistochemistry , Injections, Intraventricular , Male , Prolactin/administration & dosage , Prolactin/physiology , Rats , Rats, Sprague-Dawley , Tissue DistributionABSTRACT
Not surprisingly, since we have a need to live, grow and reproduce, evolution has equipped us with a powerful drive to seek food and devour it. If that is not enough, our hedonistic emotions have made eating and drinking a pleasurable experience. So, what tells us to stop eating? Have we evolved systems to do so effectively? The ever increasing problems of excess weight and obesity would suggest not.
Subject(s)
Appetite/physiology , Health , Biological Evolution , Body Weight , Diet , Humans , Obesity/physiopathologyABSTRACT
The hypothalamo-neurohypophysial system (HNS) is the major peptidergic neurosecretory system through which the brain controls peripheral physiology. The hormones vasopressin and oxytocin released from the HNS at the neurohypophysis serve homeostatic functions of water balance and reproduction. From a physiological viewpoint, the core question on the HNS has always been, "How is the rate of hormone production controlled?" Despite a clear description of the physiology, anatomy, cell biology, and biochemistry of the HNS gained over the last 100 years, this question has remained largely unanswered. However, recently, significant progress has been made through studies of gene identity and gene expression in the magnocellular neurons (MCNs) that constitute the HNS. These are keys to mechanisms and events that exist in the HNS. This review is an inventory of what we know about genes expressed in the HNS, about the regulation of their expression in response to physiological stimuli, and about their function. Genes relevant to the central question include receptors and signal transduction components that receive and process the message that the organism is in demand of a neurohypophysial hormone. The key players in gene regulatory events, the transcription factors, deserve special attention. They do not only control rates of hormone production at the level of the gene, but also determine the molecular make-up of the cell essential for appropriate development and physiological functioning. Finally, the HNS neurons are equipped with a machinery to produce and secrete hormones in a regulated manner. With the availability of several gene transfer approaches applicable to the HNS, it is anticipated that new insights will be obtained on how the HNS is able to respond to the physiological demands for its hormones.
Subject(s)
Gene Expression Regulation/physiology , Hypothalamo-Hypophyseal System/physiology , Animals , Humans , Promoter Regions, Genetic , Transcription Factors/physiologyABSTRACT
The neuropeptide neurotensin and two classes of its receptors, the neurotensin receptor-1 and 2, are present in the suprachiasmatic nucleus of the mammalian hypothalamus. The suprachiasmatic nucleus houses the mammalian central circadian pacemaker, but the effects of neurotensin on cellular activity in this circadian pacemaker are unknown. In this study, we examined the effects of neurotensin on the spontaneous discharge rate of rat SCN cells in an in vitro slice preparation. Neurotensin (1-10 microM) increased cell firing rate in approximately 50% of cells tested, while approximately 10% of suprachiasmatic cells tested showed a decrease in firing rate in response to neurotensin. These effects of neurotensin were not altered by the GABA receptor antagonist bicuculline (20 microM) or the glutamate receptor antagonists, D-aminophosphopentanoic acid (50 microM) and 6-cyano-7-nitroquinoxaline-2,3-dione (20 microM). The neurotensin receptor selective antagonists SR48692 and SR142948a (10 microM) failed to antagonise neurotensin responses in the majority of cells examined. Compounds that function as agonists selective for the neurotensin-receptor subtypes 1 and 2, JMV-510 and JMV-431 respectively, elicited neurotensin-like responses in approximately 90% of cells tested. Six out of seven cells tested responded to both JMV-510 and JMV-431. Neuropeptide Y (100nM) treatment of suprachiasmatic nucleus slices was found to elicit profound suppression of neuronal firing rate. Co-application of neurotensin with neuropeptide Y significantly (P<0.05) reduced the duration of the response, as compared to that elicited with neuropeptide Y alone. Together, these results demonstrate for the first time the actions of neurotensin in the suprachiasmatic nucleus and raise the possibility that this neuropeptide may play a role in modulating circadian pacemaker function.
Subject(s)
Neurons/drug effects , Neurons/physiology , Neurotensin/analogs & derivatives , Neurotensin/pharmacology , Peptide Fragments/pharmacology , Suprachiasmatic Nucleus/drug effects , Suprachiasmatic Nucleus/physiology , Animals , Drug Interactions , Electrophysiology , In Vitro Techniques , Male , Neurotransmitter Agents/physiology , Protein Isoforms/agonists , Rats , Rats, Wistar , Receptors, Neurotensin/agonists , Suprachiasmatic Nucleus/cytologyABSTRACT
Prolactin-releasing peptide (PrRP) is a peptide ligand for the human orphan G-protein-coupled receptor hGR3/GPR10 and causes the secretion of prolactin from anterior pituitary cells. However, the lack of immunoreactive staining for PrRP in the external layer of the median eminence seems to rule out this peptide as a classical hypophysiotropic hormone and, furthermore, PrRP is less effective than another inducer of prolactin secretion, thyrotropin-releasing hormone, both in vitro and in vivo. Here we show a reduction in the expression of PrRP mRNA during lactation and fasting and an acute effect of PrRP on food intake and body weight, supporting the hypothesis of an alternative role for the peptide.
Subject(s)
Eating/physiology , Hypothalamic Hormones/pharmacology , Hypothalamic Hormones/physiology , Neuropeptides/pharmacology , Neuropeptides/physiology , Receptors, Neuropeptide/physiology , Animals , Cerebral Ventricles/drug effects , Cerebral Ventricles/physiology , Fasting/physiology , Female , Humans , Hypothalamic Hormones/genetics , Injections, Intraventricular , Lactation/physiology , Neuropeptides/genetics , Prolactin-Releasing Hormone , RNA, Messenger/genetics , Rats , Receptors, Neuropeptide/genetics , Transcription, GeneticABSTRACT
Both leptin and growth hormone secretagogues are believed to have stimulatory effects on the hypothalamic growth hormone pulse generator, though whether these are achieved through the same pathway is unknown. Systemic administration of a normally maximal effective dose of the growth hormone secretagogue GHRP-6 to male rats causes the induction of c-Fos protein in the ventromedial aspect of the hypothalamic arcuate nucleus. The effect of the same dose of GHRP-6 is, however, much greater in animals that have been fasted for 48 h, suggesting that in the food-replete rat, arcuate neurons either show reduced sensitivity to endogenous growth hormone secretagogues or they are under the tonic inhibitory influences of other factors. The major populations of arcuate neurons activated by GHRP-6 have been shown to contain neuropeptide Y or growth hormone-releasing factor, while leptin is thought to be inhibitory to neuropeptide Y neurons. Leptin did not alter the response of the rats to GHRP-6. However, it was able by itself to induce c-Fos protein immunoreactivity in the ventral, including the ventrolateral, arcuate nucleus of fasted rats. This is a clear demonstration of the acute activation of arcuate neurons in the rat following systemic leptin injection and suggests that GHRP-6 and leptin act on the growth hormone axis via different pathways.
Subject(s)
Arcuate Nucleus of Hypothalamus/physiology , Fasting/physiology , Growth Hormone-Releasing Hormone/pharmacology , Hormones/pharmacology , Leptin/pharmacology , Neurons/drug effects , Oligopeptides/pharmacology , Animals , Arcuate Nucleus of Hypothalamus/cytology , Arcuate Nucleus of Hypothalamus/drug effects , Gene Expression/drug effects , Genes, fos , Immunohistochemistry , Male , Neuropeptide Y/metabolism , RatsABSTRACT
Induction of the prodynorphin gene occurs in a tissue-specific manner following different physiological stimuli. Using electrophoretic mobility shift assays, we studied the relative activity of the five major regulatory sites in the rat prodynorphin promoter. Prodynorphin cyclic AMP-responsive element 2 (DynCRE2), DynCRE3, and the noncanonical prodynorphin AP-1 (ncDynAP-1) regulatory sites control, in a coordinated manner, prodynorphin induction in the spinal cord after noxious stimulation, whereas prodynorphin up-regulation in supraoptic neurons is regulated predominantly by the ncDynAP-1. Conversely, prodynorphin transactivation in the ovaries upon gonadotropin stimulation is controlled by DynCRE1 and DynCRE3. Our results support the idea that stimulus-specific changes in nuclear proteins establish a functional hierarchy among regulatory sites in the prodynorphin promoter and provide further insight in the molecular mechanisms that govern prodynorphin gene regulation.
Subject(s)
Enhancer Elements, Genetic/genetics , Enkephalins/genetics , Promoter Regions, Genetic/genetics , Protein Precursors/genetics , Animals , Cell Nucleus/chemistry , Cyclic AMP/physiology , Cyclic AMP Response Element-Binding Protein/genetics , Electrophoresis/methods , Female , Gene Expression Regulation/drug effects , Gene Expression Regulation/genetics , Gonadotropins/pharmacology , Osmotic Pressure , Ovary/chemistry , Pain/physiopathology , Rats , Rats, Wistar , Spinal Cord/chemistry , Stimulation, Chemical , Supraoptic Nucleus/chemistry , Transcription Factor AP-1/genetics , Transcription, Genetic/physiologySubject(s)
Neurons/physiology , Nitric Oxide Synthase/metabolism , Nitric Oxide/physiology , Prosencephalon/physiology , Water-Electrolyte Balance/physiology , Animals , Axonal Transport , Cyclic GMP/metabolism , Endothelium, Vascular/metabolism , Male , Neuroglia/metabolism , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase Type I , Oxytocin/physiology , Preoptic Area/physiology , Prosencephalon/blood supply , Prosencephalon/enzymology , Proto-Oncogene Proteins c-fos/genetics , Rats , Subfornical Organ/physiology , Supraoptic Nucleus/physiology , Transcription, GeneticABSTRACT
1. Previous studies have shown that histaminergic neurones of the tuberomammillary nucleus project directly to hypothalamic magnocellular nuclei and that intracerebroventricular administration of histamine increases the synthetic activity of magnocellular oxytocin neurones. 2. Histaminergic neurones of the dorsomedial tuberomammillary nucleus that project to the magnocellular region of the paraventricular nucleus are activated during late pregnancy and lactation, as measured by an increase in mRNA for the synthetic enzyme histidine decarboxylase. 3. There is a concomitant increase in oxytocin mRNA in magnocellular neurones of the paraventricular nucleus. This increase in mRNA contributes to an accumulation of oxytocin before birth and to continued oxytocin synthesis during lactation. 4. Intracerebroventricular administration of mepyramine, a specific antagonist of the H1 histamine receptor, causes a delay in the birth of subsequent pups if given to the mother during parturition. Vehicle or the H2 receptor antagonist cimetidine has no effect. Thus, histamine acts centrally, via H1 receptors, during parturition and may have an excitatory effect on oxytocin release. 5. These results suggest that afferent histaminergic neurones show increased activity during pregnancy and may be responsible for the increase of synthesis in magnocellular oxytocin neurones at this time. If, as previously reported, these histamine neurones can reduce the electrical activity of oxytocin neurones via H2 receptors, then they may have a dual effect, increasing the synthesis of oxytocin while inhibiting its premature release. At term, any inhibitory effects of histamine are overcome to allow oxytocin secretion.
Subject(s)
Histamine/physiology , Labor, Obstetric/physiology , Neurons/physiology , Oxytocin/physiology , Pregnancy, Animal/physiology , Afferent Pathways/physiology , Animals , Cimetidine/pharmacology , Female , Histamine Antagonists/pharmacology , Histidine Decarboxylase/genetics , In Situ Hybridization , Labor, Obstetric/drug effects , Lactation/physiology , Mammillary Bodies/physiology , Oxytocin/genetics , Oxytocin/metabolism , Paraventricular Hypothalamic Nucleus/physiology , Pregnancy , Pregnancy, Animal/drug effects , Pyrilamine/pharmacology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, WistarABSTRACT
The hypothalamo-neurohypophysial system contains high levels of neuronal nitric oxide synthase and this increases further during times of neurohormone demand, such as that following osmotic stimulation. Using double in situ hybridization, we demonstrate here an increase in the expression of nitric oxide synthase messenger RNA by oxytocin neurons, but not vasopressin neurons, of the supraoptic nucleus at the time of lactation, when oxytocin is in demand due to another neuroendocrine stimulus, the milk-ejection reflex. In addition, using immunocytochemical retrograde tracing, we show that neurons of the subfornical organ, median preoptic nucleus and organum vasculosum of the lamina terminalis, which project to the supraoptic nucleus, contain nitric oxide synthase. These three structures of the lamina terminalis, together with the hypothalamo-neurohypophysial system, make up the forebrain osmoresponsive circuit that controls osmotically-stimulated release of oxytocin in the rat. The expression of nitric oxide synthase messenger RNA in the lamina terminalis was also shown to increase during lactation. The increases in nitric oxide synthase messenger RNA were not apparent during pregnancy. These results provide evidence for an integrated nitric oxide synthase-containing neural network involved in the regulation of the hypothalamo-neurohypophysial axis. The expression of nitric oxide synthase messenger RNA increases in this circuit during lactation and correlates with a reduction in the sensitivity of the circuit to osmotic stimuli also present in lactation but not pregnancy. As nitric oxide is believed to attenuate neurohormone release, it seems that the increased nitric oxide synthase messenger RNA expression detected here during lactation at a time of high oxytocin demand may be involved in reducing the sensitivity of the whole forebrain circuit to osmotic stimuli.
Subject(s)
Nitric Oxide Synthase/genetics , Oxytocin/metabolism , Paraventricular Hypothalamic Nucleus/enzymology , Supraoptic Nucleus/enzymology , Animals , Female , Fluorescent Dyes , Gene Expression Regulation, Enzymologic/physiology , Hypothalamo-Hypophyseal System/enzymology , Hypothalamo-Hypophyseal System/metabolism , In Situ Hybridization , Lactation/physiology , Microinjections , Microspheres , Neurons/chemistry , Neurons/enzymology , Neurons/metabolism , Oxytocin/analysis , Paraventricular Hypothalamic Nucleus/metabolism , Pregnancy , Prosencephalon/enzymology , Prosencephalon/metabolism , RNA, Messenger/metabolism , Rats , Rats, Wistar , Supraoptic Nucleus/metabolism , Vasopressins/analysis , Vasopressins/physiology , Water-Electrolyte Balance/physiologyABSTRACT
In this study we investigated the neurochemical identity of the arcuate cells activated following GH-releasing peptide-6 (GHRP-6) injection by comparing, on consecutive sections, the distribution c-fos messenger RNA (mRNA) with that of mRNAs for peptides synthesized in arcuate cells, including neuropeptide Y (NPY), GH-releasing factor (GRF), tyrosine hydroxylase, POMC, and somatostatin. Rats bearing chronically implanted jugular catheters were injected with either 50 micrograms GHRP-6 or vehicle. Thirty minutes later they were terminally anesthetized and perfused with fixative. Paraffin-embedded sections of 7 microns thickness were processed using in situ hybridization for either c-fos mRNA or mRNAs for the neurochemical markers. In GHRP-6-treated rats the mean (+/-SEM) number of cells expressing c-fos mRNA in the arcuate nucleus (23 +/- 2 cells/section per rat; n = 5) was significantly higher than for vehicle-treated controls (2 +/- 1 cells/section per rat; n = 5; P < 0.001, Mann-Whitney U test). Superimposed camera lucida maps indicated that, in GHRP-6-injected rats, neurochemically identifiable cells expressing c-fos mRNA also express NPY mRNA (51 +/- 4%), GRF mRNA (23 +/- 1%) tyrosine hydroxylase mRNA (11 +/- 3%), POMC mRNA (11 +/- 2%), or somatostatin mRNA (4 +/- 1%). Thus, the majority of cells expressing c-fos mRNA following GHRP-6 injection are NPY and GRF-containing cells.
Subject(s)
Arcuate Nucleus of Hypothalamus/metabolism , Genes, fos/genetics , Growth Hormone-Releasing Hormone/metabolism , Neuropeptide Y/metabolism , Oligopeptides/pharmacology , RNA, Messenger/biosynthesis , Animals , Gene Expression/drug effects , In Situ Hybridization , Male , Neurons/metabolism , Pro-Opiomelanocortin/genetics , Rats , Rats, Wistar , Tyrosine 3-Monooxygenase/geneticsABSTRACT
Hypothalamic magnocellular neurons and their afferent inputs provide a model system in which to study the regulation of inducible transcription factors in the brain in vivo. Osmotic stimulation of rats produced by graded infusions of saline at different tonicities was found to lead to the induction of c-fos, nur77 and egr1 mRNAs in magnocellular neurons, as well as in putative afferent neurons, including those in structures of the forebrain (subfornical organ, median preoptic nucleus and organum vasculosum of the lamina terminalis). The results presented suggest that stronger levels of osmotic stimulation recruit additional afferents from the forebrain and brainstem that can act on magnocellular neurons via alternative receptors. A single systemic injection of the peptide cholecystokinin produced robust induction of c-fos and nur77 mRNAs in afferent neurons of the brainstem nucleus tractus solitarii and in magnocellular neurons. Despite the fact that these two neuronal populations are clearly electrically active, egr1 was not induced by this stimulus, providing examples of cell- and stimulus-specificity of its expression. This study re-emphasizes that the induction of transcription factors is largely dependent on the nature of the afferent input and does not correlate necessarily to the electrical activity of the neuron.
Subject(s)
DNA-Binding Proteins/biosynthesis , Hypothalamus/metabolism , Immediate-Early Proteins , Neurons/metabolism , Proto-Oncogene Proteins c-fos/biosynthesis , RNA, Messenger/biosynthesis , Receptors, Steroid/biosynthesis , Transcription Factors/biosynthesis , Animals , Cell Count , Cholecystokinin/pharmacology , Early Growth Response Protein 1 , Hypothalamus/cytology , Immunohistochemistry , In Situ Hybridization , Male , Neural Pathways/cytology , Neural Pathways/physiology , Neurons, Afferent/metabolism , Nuclear Receptor Subfamily 4, Group A, Member 1 , Rats , Rats, Wistar , Receptors, Cytoplasmic and Nuclear , Saline Solution, Hypertonic , Solitary Nucleus/cytology , Solitary Nucleus/metabolismABSTRACT
Osmotically stimulated vasopressin and oxytocin release were measured in pinealectomized and sham operated male rats infused with hypertonic sodium chloride. Neuronal activation in the hypothalamic regions associated with oxytocin and vasopressin release was investigated by quantitative assessment of Fos protein production. The osmotically stimulated release of both vasopressin and oxytocin was significantly lower in pinealectomized animals as compared to sham operated controls. The slope of regression lines between plasma osmolality and hormone concentrations in the sham animals showed a 1.0 +/- 0.1 pmol per mosm/kg rise in vasopressin and 2.0 +/- 0.4 pmol per mosm/kg rise in oxytocin whilst in the pinealectomized animals these values were significantly lower at 0.4 +/- 0.1 pmol vasopressin per mosm/kg and 0.8 +/- 0.2pmol oxytocin per mosm/kg. The osmotic thresholds for hormone release were unaffected by pinealectomy. Fos production was also significantly lower in the supraoptic nucleus and organ vasculosum of the lamina terminalis in the pinealectomized rat at 62 +/- 20 and 59 +/- 9 Fos immunoreactive cells/section as compared to corresponding values of 202 +/- 31 and 123 +/- 20 Fos immunoreactive cells/section in the shams. These observations suggest that reduced hormone release in the pinealectomized animal is due to lowered responsiveness of central osmoregulatory mechanisms and that melatonin may therefore influence the activation of the magnocellular system.