Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
3.
J Med Microbiol ; 56(Pt 1): 52-55, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17172517

ABSTRACT

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla(CTX-M) genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on beta-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum beta-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla(CTX-M) genes in 21 of 28 ESBL-producing isolates.


Subject(s)
Enterobacteriaceae Infections/microbiology , Enterobacteriaceae/genetics , Polymerase Chain Reaction/methods , beta-Lactamases/genetics , Ampicillin/pharmacology , Anti-Bacterial Agents/pharmacology , Cephalexin/pharmacology , DNA, Bacterial/chemistry , DNA, Bacterial/isolation & purification , Enterobacteriaceae/drug effects , Enterobacteriaceae/enzymology , Enterobacteriaceae Infections/blood , Enterobacteriaceae Infections/urine , Escherichia coli Proteins/genetics , Genotype , Humans , Microbial Sensitivity Tests , Reproducibility of Results , Sequence Analysis, DNA , beta-Lactam Resistance/genetics , beta-Lactamases/classification , beta-Lactamases/metabolism , beta-Lactams/pharmacology
SELECTION OF CITATIONS
SEARCH DETAIL
...