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1.
Occup Med (Lond) ; 71(4-5): 189-195, 2021 08 20.
Article in English | MEDLINE | ID: mdl-33963871

ABSTRACT

BACKGROUND: Presenteeism among nurses is the common behaviour of being physically present at work when one should not be due to personal health and well-being, a stressful work environment, lack of work-life balance, or a sense of professional identity or obligation. AIMS: To explore nurses' decision-making process related to presenteeism in a large Hong Kong public hospital. METHODS: As part of a larger study on nurse presenteeism in Hong Kong, we conducted focus groups with nurses to understand their considerations related to working sick. RESULTS: Eleven major themes emerged from the focus groups, which we grouped into three organizational domains reflecting nurses' decision-making process around presenteeism: consideration for colleagues, organizational factors and professional identity. CONCLUSIONS: Presenteeism was a familiar behaviour among Hong Kong nurses in our study. We found both overlap and divergence with prior presenteeism research in the domains and themes identified. Loyalty to colleagues, written and unwritten rules and professional identity as nurses all seemed to encourage presenteeism among Hong Kong nurses. Organizations seeking to discourage presenteeism should evaluate policies such as sick leave certificates, ensure appropriate coverage for all nurse ranks and address subcultural norms encouraging presenteeism.


Subject(s)
Nurses , Presenteeism , Hong Kong , Hospitals, Public , Humans , Workplace
2.
Horm Metab Res ; 48(11): 737-744, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27589347

ABSTRACT

Nutritional excess of vitamin A, a precursor for retinoic acid (RA), causes premature epiphyseal fusion, craniosynostosis, and light-dependent retinopathy. Similarly, homozygous loss-of-function mutations in CYP26B1, one of the major RA-metabolizing enzymes, cause advanced bone age, premature epiphyseal fusion, and craniosynostosis. In this paper, a patient with markedly accelerated skeletal and dental development, retinal scarring, and autism-spectrum disease is presented and the role of retinoic acid in longitudinal bone growth and skeletal maturation is reviewed. Genetic studies were carried out using SNP array and exome sequencing. RA isomers were measured in the patient, family members, and in 18 age-matched healthy children using high-performance liquid chromatography coupled to tandem mass spectrometry. A genomic SNP array identified a novel 8.3 megabase microdeletion on chromosome 10q23.2-23.33. The 79 deleted genes included CYP26A1 and C1, both major RA-metabolizing enzymes. Exome sequencing did not detect any variants that were predicted to be deleterious in the remaining alleles of these genes or other known retinoic acid-metabolizing enzymes. The patient exhibited elevated plasma total RA (16.5 vs. 12.6±1.5 nM, mean±SD, subject vs. controls) and 13-cisRA (10.7 nM vs. 6.1±1.1). The findings support the hypothesis that elevated RA concentrations accelerate bone and dental maturation in humans. CYP26A1 and C1 haploinsufficiency may contribute to the elevated retinoic acid concentrations and clinical findings of the patient, although this phenotype has not been reported in other patients with similar deletions, suggesting that other unknown genetic or environmental factors may also contribute.


Subject(s)
Bone Diseases, Developmental/pathology , Cytochrome P450 Family 26/genetics , Retinoic Acid 4-Hydroxylase/genetics , Tretinoin/metabolism , Bone Diseases, Developmental/genetics , Child , Chromosomes, Human, Pair 10/genetics , Gene Expression Regulation, Enzymologic , Humans , Male , Phenotype , Polymorphism, Single Nucleotide/genetics
3.
J Psychiatr Ment Health Nurs ; 23(5): 303-11, 2016 Jun.
Article in English | MEDLINE | ID: mdl-27307261

ABSTRACT

WHAT IS KNOWN ON THE SUBJECT?: Frailty and multimorbidity are common in later life. A higher level of frailty is associated with a higher risk of adverse physical and psychological health situations. Older adults with pain have been reported to be lonelier and more depressed, as well as less happy and less satisfied with their life as compared to those without pain. In view of the high prevalence of pain among older adults and the reversibility of frailty, it is important to explore the relationship between pain, frailty and psychological parameters in order to devise patient-centred interventions. WHAT THIS PAPER ADDS TO EXISTING KNOWLEDGE?: Frailty index is positively correlated with the presence of pain, and associated with gender, functional mobility and loneliness. Among these significant variables, loneliness was the factor that contributed the most to the frailty index. WHAT ARE THE IMPLICATIONS FOR PRACTICE?: It is essential to put the focus of healthcare on both the physical and psychological aspects of well-being. All nurses are advised to improve the management of pain in older people in order to lower the levels of pain, frailty and psychological distress among this population. Nursing care should address the loneliness level especially the problem of social loneliness among older adults particularly those living in nursing homes. ABSTRACT: Introduction In view of the high prevalence of pain among older adults and the reversibility of frailty, it is important to explore the relationship between pain, frailty and psychological parameters in order to devise patient-centred interventions. Aim To examine the levels of frailty, pain and psychological parameters among older adults living in Hong Kong nursing homes, and the cross-sectional relationships among these items. Methods A cross-sectional study was conducted among 178 residents from six nursing homes. Frailty, pain, mobility, happiness, loneliness and life satisfaction of participants were assessed using validated questionnaires. Results A multiple linear regression (R(2)  = 0.338, P < 0.05) showed that the frailty index was associated with loneliness, functional mobility and gender. Among these significant variables, loneliness was the factor that contributed the most to the frailty index. Discussion It is essential to put the focus of healthcare on both the physical and psychological aspects of well-being. Findings suggest that apart from improving mobility and reducing pain, loneliness could be a target of psychosocial interventions to reduce frailty and improve quality of life. Implications for practice It is advised that nursing care should address loneliness, especially the problem of social loneliness among older adults particularly those living in nursing homes.


Subject(s)
Comorbidity , Frail Elderly/psychology , Homes for the Aged , Loneliness/psychology , Nursing Homes , Pain/psychology , Aged , Aged, 80 and over , Cross-Sectional Studies , Female , Hong Kong , Humans , Male
4.
Spinal Cord ; 53(4): 252-64, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25582713

ABSTRACT

STUDY DESIGN: Systematic review. OBJECTIVES: To systematically review the literature on chemodenervation with botulinum toxin (BoNT) or phenol/alcohol for treatment of limb spasticity following spinal cord injury (SCI). SETTING: British Columbia, Canada. METHODS: EMBASE, MEDLINE, CINAHL, Cochrane Database of Systematic Reviews and Cochrane Central Register of Controlled Trials were searched for English language studies published up until March 2014. Studies were assessed for eligibility and quality by two independent reviewers. RESULTS: No controlled trials were identified. A total of 19 studies were included: 9 involving BoNT and 10 involving phenol/alcohol. Owing to the clinically diverse nature of the studies, meta-analysis was deemed inappropriate. The studies produced level 4 and level 5 evidence that chemodenervation with BoNT or alcohol/phenol can lead to improvement in outcome measurements classified in the body structure and function, as well as activity domains of the International Classification of Functioning, Disability and Health framework. The Modified Ashworth Scale (MAS) was the most commonly used outcome measure. All six studies on BoNT and three of the four studies on phenol/alcohol measuring MAS reported a decrease in at least one point. An improvement in MAS was not always associated with improvement in function. The effect of phenol/alcohol has the potential to last beyond 6 months; study follow-up did not occur beyond this time point. CONCLUSION: Chemodenervation with BoNT or phenol/alcohol may improve spasticity and function in individuals with SCI. However, there is a lack of high-quality evidence and further research is needed to confirm the efficacy of these interventions.


Subject(s)
Muscle Spasticity/drug therapy , Muscle Spasticity/physiopathology , Nerve Block , Spinal Cord Injuries/physiopathology , Anesthetics/adverse effects , Anesthetics/therapeutic use , Botulinum Toxins, Type A/adverse effects , Botulinum Toxins, Type A/therapeutic use , Clinical Studies as Topic , Ethanol/adverse effects , Ethanol/therapeutic use , Humans , Muscle Spasticity/etiology , Nerve Block/adverse effects , Nerve Block/methods , Phenol/adverse effects , Phenol/therapeutic use , Spinal Cord Injuries/complications
5.
Oncogene ; 34(38): 4964-74, 2015 Sep 17.
Article in English | MEDLINE | ID: mdl-25531327

ABSTRACT

Melanoma is a highly aggressive disease that is difficult to treat owing to rapid tumor growth, apoptotic resistance and high metastatic potential. The MET proto-oncogene (MET) tyrosine kinase receptor promotes many of these cellular processes, but while MET is often overexpressed in melanoma, the mechanism driving this overexpression is unknown. As the MET gene is rarely mutated or amplified in melanoma, MET overexpression may be driven to increased activation through promoter elements. In this report, we find that transcription factors PAX3 and ETS1 directly interact to synergistically activate MET expression. Inhibition of PAX3 and ETS1 expression in melanoma cells leads to a significant reduction of MET receptor levels. The 300-bp 5' proximal MET promoter contains a PAX3 response element and two ETS1 consensus motifs. Although ETS1 can moderately activate both of these sites without cofactors, robust MET promoter activation of the first site is PAX dependent and requires the presence of PAX3, whereas the second site is PAX independent. The induction of MET by ETS1 via this second site is enhanced by hepatocyte growth factor-dependent ETS1 activation, thereby MET indirectly promotes its own expression. We further find that expression of a dominant-negative ETS1 reduces the ability of melanoma cells to grow both in culture and in vivo. Thus, we discover a pathway where ETS1 advances melanoma through the expression of MET via PAX-dependent and -independent mechanisms.


Subject(s)
Melanoma/metabolism , Paired Box Transcription Factors/metabolism , Proto-Oncogene Protein c-ets-1/metabolism , Proto-Oncogene Proteins c-met/metabolism , Skin Neoplasms/metabolism , Animals , Cell Line , Cell Line, Tumor , Hepatocyte Growth Factor/metabolism , Humans , Melanoma/pathology , Mice , PAX3 Transcription Factor , Promoter Regions, Genetic , Proto-Oncogene Mas , Proto-Oncogene Proteins c-met/genetics , Skin Neoplasms/pathology
6.
Eur J Dent Educ ; 18(1): 51-7, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24423176

ABSTRACT

Training dentists today is challenging as they are expected to provide a wide range of dental care. In the provision of good dental care, soft skills are equally important as clinical skills. Therefore in dental education the development of soft skills are of prime concern. This study sought to identify the development of soft skills when dental students are paired in their clinical training. In this perception study, four open-ended items were used to elicit students' feedback on the appropriateness of using clinical pairing as an instructional strategy to promote soft skills. The most frequently cited soft skills were teamwork (70%) and communication (25%) skills. However, both negative and positive behaviours were reported. As for critical thinking and problem solving skills, more positive behaviours were reported for abilities such as to explain, analyze, find ideas and alternative solutions, and make decisions. Leadership among peers was not evident as leading without legitimate authority could be a hindrance to its development. If clinical pairing is to be used as an effective instructional strategy to promote soft skills amongst students, clear guidelines need to be developed to prepare students to work in a dental team and the use of appropriate assessment tools can facilitate the development of these soft skills.


Subject(s)
Clinical Competence , Education, Dental/methods , Communication , Decision Making , Ethics, Dental , Female , Humans , Leadership , Male , Morals , Patient Care Team , Problem Solving , Surveys and Questionnaires , Thinking
7.
Comput Math Methods Med ; 2013: 624683, 2013.
Article in English | MEDLINE | ID: mdl-23606904

ABSTRACT

A segmentation algorithm to isolate areas of ventilation from hyperpolarized helium-3 magnetic resonance imaging (HP (3)He MRI) is described. The algorithm was tested with HP (3)He MRI data from four healthy and six asthmatic subjects. Ventilated lung volume (VLV) measured using our semiautomated technique was compared to that obtained from manual outlining of ventilated lung regions and to standard spirometric measurements. VLVs from both approaches were highly correlated (R = 0.99; P < 0.0001) with a mean difference of 3.8 mL and 95% agreement indices of -30.8 mL and 38.4 mL. There was no significant difference between the VLVs obtained through the semiautomatic approach and the manual approach. A Dice coefficient which quantified the intersection of the two datasets was calculated and ranged from 0.95 to 0.97 with a mean of 0.96 ± 0.01 (mean ± SD). VLVs obtained through the semiautomatic algorithm were also highly correlated with measurements of forced expiratory volume in one second (FEV1) (R = 0.82; P = 0.0035) and forced vital capacity (FVC) (R = 0.95; P < 0.0001). The technique may open new pathways toward advancing more quantitative characterization of ventilation for routine clinical assessment for asthma severity as well as a number of other respiratory diseases.


Subject(s)
Asthma/physiopathology , Magnetic Resonance Imaging/methods , Adult , Algorithms , Case-Control Studies , Computational Biology , Female , Forced Expiratory Volume , Helium , Humans , Image Interpretation, Computer-Assisted , Isotopes , Lung/physiopathology , Magnetic Resonance Imaging/statistics & numerical data , Male , Respiratory Function Tests/methods , Respiratory Function Tests/statistics & numerical data , Spirometry , Young Adult
8.
World Rev Nutr Diet ; 106: 100-4, 2013.
Article in English | MEDLINE | ID: mdl-23428687

ABSTRACT

In mammals, after a period of growth inhibition, body growth often does not just return to a normal rate but actually exceeds the normal rate, resulting in catch-up growth. Recent evidence suggests that catch-up growth occurs because growth-inhibiting conditions delay progression of the physiological mechanisms that normally cause body growth to slow and cease with age. As a result, following the period of growth inhibition, tissues retain a greater proliferative capacity than normal, and therefore grow more rapidly than normal for age. There is evidence that this mechanism contributes both to catch-up growth in terms of body length, which involves proliferation in the growth plate, and to catch-up growth in terms of organ mass, which involves proliferation in multiple nonskeletal tissues.


Subject(s)
Growth Plate/cytology , Growth Plate/growth & development , Animals , Body Height , Cell Proliferation , Chondrocytes/cytology , Chondrocytes/metabolism , Humans , Models, Animal
9.
Mol Psychiatry ; 18(8): 875-81, 2013 Aug.
Article in English | MEDLINE | ID: mdl-22889922

ABSTRACT

Previous studies suggest physical activity improves cognition and lowers Alzheimer's disease (AD) risk. However, key AD pathogenic factors that are thought to be influenced by physical activity, particularly plasma amyloid-ß (Aß) and Aß brain load, have yet to be thoroughly investigated. The objective of this study was to determine if plasma Aß and amyloid brain deposition are associated with physical activity levels, and whether these associations differed between carriers and non-carriers of the apolipoprotein E (APOE) ε4 allele. Five-hundred and forty six cognitively intact participants (aged 60-95 years) from the Australian Imaging, Biomarkers and Lifestyle Study of Ageing (AIBL) were included in these analyses. Habitual physical activity levels were measured using the International Physical Activity Questionnaire (IPAQ). Serum insulin, glucose, cholesterol and plasma Aß levels were measured in fasting blood samples. A subgroup (n=116) underwent (11)C-Pittsburgh compound B (PiB) positron emission tomography (PET) scanning to quantify brain amyloid load. Higher levels of physical activity were associated with higher high density lipoprotein (HDL) (P=0.037), and lower insulin (P<0.001), triglycerides (P=0.019) and Aß1-42/1-40 ratio (P=0.001). After stratification of the cohort based on APOE ε4 allele carriage, it was evident that only non-carriers received the benefit of reduced plasma Aß from physical activity. Conversely, lower levels of PiB SUVR (standardised uptake value ratio) were observed in higher exercising APOE ε4 carriers. Lower plasma Aß1-42/1-40 and brain amyloid was observed in those reporting higher levels of physical activity, consistent with the hypothesis that physical activity may be involved in the modulation of pathogenic changes associated with AD.


Subject(s)
Aging/metabolism , Amyloid beta-Peptides/metabolism , Brain/metabolism , Motor Activity , Aged , Aged, 80 and over , Aging/genetics , Alleles , Amyloid beta-Peptides/blood , Apolipoprotein E4/genetics , Biomarkers/blood , Biomarkers/metabolism , Blood Glucose , Cholesterol/blood , Female , Functional Neuroimaging , Humans , Insulin/blood , Life Style , Male , Middle Aged
10.
J Dent ; 41 Suppl 3: e53-61, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23103847

ABSTRACT

OBJECTIVES: This study was to compare the effect of three different one-step polishing systems on the color stability of three different types of nanocomposites after immersion in coffee for one day and seven days and determine which nanocomposite material has the best color stability following polishing with each of the one-step polishing system. METHODS: The nanocomposites tested were Tetric EvoCeram, Grandio and Herculite Précis. A total of 120 discs (40/nanocomposite, 8mm×2mm) were fabricated. Ten specimens for each nanocomposite cured under Mylar strips served as the control. The other specimens were polished with OptraPol, OneGloss and Occlubrush immersed in coffee (Nescafé) up to seven days. Color measurements were made with a spectrophotometer at baseline and after one and seven days. Two way repeated measure ANOVA, two way ANOVA and Bonferroni tests were used for statistical analyses (P<0.05). RESULTS: The immersion time was a significant factor in the discoloration of the nanocomposites. The effect of three one-step polishing systems on the color stability was also significant. The color change values of the materials cured against Mylar strips were the greatest. The lowest mean color change values were from the Occlubrush polished groups. The effect of the three different types of nanocomposite on the color change was significant. The highest color change values were with Tetric EvoCeram groups. The lowest color change values were with Herculite Précis groups. CONCLUSION: The color change of nanocomposite resins is affected by the type of composite, polishing procedure and the period of immersion in the staining agent.


Subject(s)
Composite Resins/chemistry , Dental Materials/chemistry , Dental Polishing/methods , Nanocomposites/chemistry , Aluminum Oxide/chemistry , Bisphenol A-Glycidyl Methacrylate/chemistry , Carbon Compounds, Inorganic/chemistry , Coffee , Color , Dental Polishing/instrumentation , Hemiterpenes/chemistry , Humans , Immersion , Latex/chemistry , Materials Testing , Polyethylene Glycols/chemistry , Polymerization , Polymethacrylic Acids/chemistry , Polyvinyls/chemistry , Silicon Compounds/chemistry , Silicon Dioxide/chemistry , Siloxanes/chemistry , Spectrophotometry/instrumentation , Surface Properties , Time Factors , Titanium/chemistry
11.
J Perinatol ; 32(10): 791-6, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22222549

ABSTRACT

OBJECTIVE: We previously reported that preterm mothers' milk production can exceed levels of term mothers by using early hand expression and hands-on pumping (HOP) with the highest production (955 ml per day) in frequent users of hand expression. In this study, we compared milk composition between mothers stratified by early hand expression frequency. STUDY DESIGN: A total of 67 mothers of infants <31 weeks gestation were instructed on hand expression and HOP. Subjects submitted expression records and 1-ml samples from each pumping session over 24 h once weekly for 8 weeks. RESULT: 78% (52/67) of mothers completed the study. But for Week 1, no compositional differences (despite production differences) were noted between the three groups. Protein and lactose tracked reported norms, but fat and energy of mature milk (Weeks 2-8) exceeded norms, 62.5 g l(-1) per fat and 892.7 cal l(-1) (26.4 cal oz(-1)), respectively. CONCLUSION: Mothers combining manual techniques with pumping express high levels of fat-rich, calorie-dense milk, unrelated to production differences.


Subject(s)
Breast Milk Expression/methods , Infant, Premature , Milk, Human/chemistry , Energy Intake , Female , Humans , Infant, Newborn , Mothers
12.
Genet Mol Res ; 10(4): 2860-6, 2011 Oct 31.
Article in English | MEDLINE | ID: mdl-22057997

ABSTRACT

We evaluated genetic and environmental factors affecting age at first farrowing of sows in the Brazilian southeast. For this purpose, 466 observations regarding the age at first farrowing were made for Dalland-C40© animals belonging to two herds. The effects of the environmental factors on this trait were assessed by means of a model that included, as random effects, the influence of the sow's father and mother and, as fixed effects, the influence the year of birth, the herd and the birth season, along with the covariable litter size at birth. The variance components were estimated using the derivative-free restricted maximum likelihood method. The estimated mean was 354.8 ± 25.87 days, with a coefficient of variation of 7.29%. Significant effects on the trait were observed for the herd, the year and the season of birth; but a linear effect of litter size at birth on the age at first farrowing was not observed. The boar did not significantly contribute to the variation occurring among the sows, whereas the sow's mother caused significant variation. The heritability estimate for the age at first farrowing was 0.44 ± 0.15, which is considered high. We concluded that herd effect and year and season of birth should be taken into consideration for an accurate genetic comparison; consequently, the animals should be joined into contemporary groups.


Subject(s)
Parturition/genetics , Reproduction/genetics , Age Factors , Analysis of Variance , Animals , Brazil , Breeding , Climate , Female , Litter Size/genetics , Models, Statistical , Parity/genetics , Pregnancy , Seasons , Swine
13.
Genet Mol Res ; 10(4): 2692-701, 2011 Nov 04.
Article in English | MEDLINE | ID: mdl-22095595

ABSTRACT

We examined the effects of environmental and genetic factors on the weaning-to-estrus interval (WEI) in sows. In order to perform the analyses of the environmental factors, 8104 observations of the 1st to the 6th WEI were carried out, while 6548 observations of the 1st to the 3rd WEI were carried out for the analyses of genetic factors. The environmental model included as fixed effects, herd, genetic line, year and season of birth, as well as the covariates, age of sow at farrowing, litter size at birth and lactation length. Genetic analysis was performed by repeatability and multitrait models. The mean and coefficient of variation for WEI were 7.02 days and 100.6%, respectively. The linear effect of lactation length and the quadratic effect of the age of sow at farrowing affected the WEI. Herd, year and season of farrowing were significant sources of variation for WEI, and there was no influence of genetic line or of litter size at birth. Heritability estimated by the repeatability model was 0.04, while heritabilities obtained by the multitrait model were 0.07, 0.02 and 0.07 for the first three WEI, respectively. Estimates of genetic correlations among the different WEI were of moderate to low magnitude. It was concluded that environmental factors, such as year and season of farrowing, lactation length, age of sow at farrowing and herd, should be considered in the model for best estimation of genetic parameters for this trait. Although with only a small possible genetic gain, selection can be made based on the first WEI.


Subject(s)
Estrous Cycle/physiology , Gene-Environment Interaction , Models, Biological , Quantitative Trait Loci/physiology , Swine/physiology , Weaning , Animals , Female , Lactation/physiology
14.
Neurology ; 76(12): 1091-8, 2011 Mar 22.
Article in English | MEDLINE | ID: mdl-21422459

ABSTRACT

OBJECTIVE: There is mounting evidence for the contribution of apoE to the pathophysiology of Alzheimer disease (AD). Studies also indicate that plasma apoE levels may reflect disease status, suggesting that apoE is a potential AD biomarker. However, while some studies of apoE levels in plasma have presented correlations with AD pathology, others have not. Thus, there is a lack of consensus as to the suitability of plasma apoE as an AD biomarker. The major objective of this cross-sectional study was to investigate total plasma apoE as well as levels of the apoE4 form in a large, highly characterized cohort which included both healthy controls and participants with early-stage AD. METHODS: Total apoE and apoE4 were measured in 1,079 individuals drawn from the highly characterized Australian Imaging, Biomarkers and Lifestyle (AIBL) study. Total and isoform-specific plasma apoE levels were then compared with cerebral Aß load, as assessed by PET using Pittsburgh compound B (PiB). RESULTS: Total apoE and apoE4 levels were found to be significantly lower in patients with AD in the entire cohort, and decrease with Aß load in the PiB-PET subset. ApoE levels were significantly lower among ε4 homozygous individuals. In APOE ε3/ε4 heterozygote carriers, apoE4 levels decrease, indicating that apoE3 levels increase with disease. CONCLUSION: Analysis of cross-sectional data from the AIBL study indicates that plasma apoE levels are altered in AD and correlate with AD pathology level. The significance of these findings will be determined in the AIBL longitudinal study of aging.


Subject(s)
Aging/metabolism , Alzheimer Disease/diagnosis , Apolipoprotein E4/blood , Apolipoproteins E/blood , Brain/metabolism , Cognition Disorders/diagnosis , Positron-Emission Tomography/methods , Aged , Aging/blood , Aging/psychology , Alzheimer Disease/blood , Alzheimer Disease/complications , Alzheimer Disease/diagnostic imaging , Amyloid beta-Peptides/metabolism , Aniline Compounds , Biomarkers/blood , Brain/diagnostic imaging , Carbon Radioisotopes , Cognition Disorders/blood , Cognition Disorders/complications , Cognition Disorders/diagnostic imaging , Cross-Sectional Studies , Heterozygote , Homozygote , Humans , Risk Factors , Thiazoles
15.
J Appl Microbiol ; 110(6): 1571-83, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21447014

ABSTRACT

AIMS: Research into the relationship between pathogens, faecal indicator microbes and environmental factors in beach sand has been limited, yet vital to the understanding of the microbial relationship between sand and the water column and to the improvement of criteria for better human health protection at beaches. The objectives of this study were to evaluate the presence and distribution of pathogens in various zones of beach sand (subtidal, intertidal and supratidal) and to assess their relationship with environmental parameters and indicator microbes at a non-point source subtropical marine beach. METHODS AND RESULTS: In this exploratory study in subtropical Miami (Florida, USA), beach sand samples were collected and analysed over the course of 6 days for several pathogens, microbial source tracking markers and indicator microbes. An inverse correlation between moisture content and most indicator microbes was found. Significant associations were identified between some indicator microbes and pathogens (such as nematode larvae and yeasts in the genus Candida), which are from classes of microbes that are rarely evaluated in the context of recreational beach use. CONCLUSIONS: Results indicate that indicator microbes may predict the presence of some of the pathogens, in particular helminthes, yeasts and the bacterial pathogen Staphylococcus aureus including methicillin-resistant forms. Indicator microbes may thus be useful for monitoring beach sand and water quality at non-point source beaches. SIGNIFICANCE AND IMPACT OF THE STUDY: The presence of both indicator microbes and pathogens in beach sand provides one possible explanation for human health effects reported at non-point sources beaches.


Subject(s)
Bacteria/isolation & purification , Bathing Beaches , Environmental Monitoring/methods , Helminths/isolation & purification , Water Microbiology , Yeasts/isolation & purification , Animals , Colony Count, Microbial , Florida , Seawater/microbiology , Seawater/parasitology , Silicon Dioxide/analysis
16.
J Periodontal Res ; 46(1): 89-96, 2011 Feb.
Article in English | MEDLINE | ID: mdl-20860592

ABSTRACT

BACKGROUND AND OBJECTIVE: In recent years, there has been a growing interest in the use of dental lasers for treatment of periodontal diseases. The purpose of this short-term clinical trial was to evaluate the effects of a combination of photodynamic therapy with low-level laser therapy as an adjunct to nonsurgical treatment of chronic periodontitis. MATERIAL AND METHODS: Twenty-four nonsmoking adults with untreated chronic periodontitis were randomly assigned in a split-mouth design to receive scaling and root debridement with or without one course of adjunctive photodynamic therapy and low-level laser therapy within 5 d. Plaque, bleeding on probing, probing depth and gingival recession were recorded at baseline, 1 and 3 mo after the treatment. Gingival crevicular fluid was collected for assay of interleukin-1ß levels at baseline, 1 wk and 1 mo. RESULTS: The test teeth achieved greater reductions in the percentage of sites with bleeding on probing and in mean probing depth at 1 mo compared with the control teeth (p < 0.05). A significant decrease in gingival crevicular fluid volume was observed in both groups at 1 wk (p < 0.001), with a further decrease at 1 mo in the test sites (p < 0.05). The test sites showed a greater reduction of interleukin-1ß levels in gingival crevicular fluid at 1 wk than the control sites (p < 0.05). No significant differences in periodontal parameters were found between the test and control teeth at 3 mo. CONCLUSIONS: The present study suggests that a combined course of photodynamic therapy with low-level laser therapy could be a beneficial adjunct to nonsurgical treatment of chronic periodontitis on a short-term basis. Further studies are required to assess the long-term effectiveness of the combination of photodynamic therapy with low-level laser therapy as an adjunct in nonsurgical treatment of periodontitis.


Subject(s)
Chronic Periodontitis/drug therapy , Chronic Periodontitis/radiotherapy , Low-Level Light Therapy , Photochemotherapy , Adult , Combined Modality Therapy , Dental Scaling , Female , Gingival Crevicular Fluid/chemistry , Humans , Interleukin-1beta/analysis , Male , Middle Aged , Periodontal Debridement , Single-Blind Method , Treatment Outcome
17.
Clin Genet ; 75(3): 220-4, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19263514

ABSTRACT

It is often recommended that women who carry a mutation in the BRCA1 or BRCA2 gene have their ovaries and fallopian tubes removed to reduce their risk of gynecologic cancer. The aim of this study was to evaluate women's perception of their risk of breast and ovarian cancer before and after prophylactic salpingo-oophorectomy. We surveyed 127 women who carry a BRCA1 or BRCA2 mutation and who underwent prophylactic salpingo-oophorectomy at the University Health Network, Toronto. Subjects were asked to estimate their risks of breast and ovarian cancer before and after surgery. Their perceived risks of cancers were then compared with published risks, based on their mutation status. BRCA1 carriers estimated their risk of breast cancer risk to be, on average, 69% before surgery and 41% after surgery. They estimated their risk of ovarian cancer to be 55% before surgery and 11% after surgery. BRCA2 carriers estimated their risk of breast cancer to be 69% prior to surgery and 45% after surgery and their perceived risk of ovarian cancer to be 43% before surgery and 8% after surgery. Compared with published risk figures, the perceived risk of ovarian cancer before prophylactic salpingo-oophorectomy was overestimated by 47% of BRCA1 mutation carriers and by 61% of BRCA2 mutation carriers. Most women who have undergone genetic counseling and subsequently choose prophylactic salpingo-oophorectomy accurately perceive their risk of breast cancer. However, in this study, many women overestimated their risk of ovarian cancer, particularly women who carry a BRCA2 mutation.


Subject(s)
Breast Neoplasms/prevention & control , Genes, BRCA1 , Genes, BRCA2 , Genetic Counseling , Mutation , Ovarian Neoplasms/prevention & control , Adult , Aged , Breast Neoplasms/genetics , Breast Neoplasms/psychology , Female , Genetic Predisposition to Disease , Humans , Middle Aged , Ovarian Neoplasms/genetics , Ovarian Neoplasms/psychology , Ovariectomy , Perception , Risk Factors
18.
Arq. bras. med. vet. zootec ; 61(1): 280-285, fev. 2009.
Article in Portuguese | LILACS | ID: lil-513053

ABSTRACT

The effect of environmental and genetic effects on the farrowing interval in Dalland (C-40) sows in the Southeast of Brazil was studied. Data consisting of 1,013 farrowing intervals recorded in two herds were analyzed, using a model that included the sire and the dam as random effects and the year of farrowing, the herd and the farrowing season as fixed effects, plus the covariables sow's age at farrowing, litter size at birth, lactation length and weaning-estrus interval. For the farrowing interval first only, variance components were estimated by REML, with an animal model that included, as fixed effect, a contemporary group and, as random effects, the additive genetic variance and the error. The mean farrowing interval was 140.9+5.7 days, with a 4.0 percent coefficient of variation. Variance analysis showed no effect of either year, season of farrowing or herd on the farrowing interval. The sire effect was not important for the farrowing interval, but the dam represented an important source of variation. The total number of piglets born and the sow's age at farrowing had no influence on the farrowing interval. The length of lactation exerted an influence on the farrowing interval, accounting for 19.4 percent of the total variation of this trait. Likewise, the linear regression of the weaning-estrus interval in relation to the farrowing interval was highly significant, accounting for 51.7 percent of the total variation. The heritability estimate was 0.00, suggesting that no genetic gain can be obtained by selection for a shorter farrowing interval.


Subject(s)
Animals , Female , Genetics , Pregnancy, Animal , Reproduction , Swine
19.
Arq. bras. med. vet. zootec ; 60(2): 499-502, abr. 2008.
Article in Portuguese | LILACS | ID: lil-484682

ABSTRACT

The genetic and environmental factors that affect age at first conception (AFC) of gilts were evaluated, using 466 observations of Dalland animals (C-40). The software program MTDFREML was used to assess the genetic parameters on an animal model that included as fixed effect, contemporary group and, as random effects, the additive genetic, the common litter and the error. Mean value of AFC was 241.12±25.9 days, with CV of 8.5 percent. Variance analysis showed that year (P<0.01), season (P<0.01) and herd effects (P<0.01) were important sources of variation of AFC. The throat effect (P=0.34) was not important for the AFC, but the mother of the gilts (P<0.01) was an important source of variation. The heritability estimate was 0.44±0.14 that this trait would yield genetic gain as a response to the selection.


Subject(s)
Animals , Female , Fertilization , Reproduction , Swine
20.
Meat Sci ; 80(4): 1200-4, 2008 Dec.
Article in English | MEDLINE | ID: mdl-22063857

ABSTRACT

The aim of this study was to compare wild boar (chromosomal number 2n=36) to phenotypically similar animals of 2n=37 and 2n=38 chromosomes (crossbreeds) with respect to live weight, carcass yield, meat yield, fat and weight of inner organs. All animals were born and raised on the same farm and slaughtered at 39 weeks. The final live weight of wild boar 2n=36 was significantly lower (47.2kg) as compared to crossbreeds (80.0kg). Animals 2n=36 had more carcass yields (65.5%) than 2n=37 karyotype (64.9%) and 2n=38 (64.4%). Wild boar had the highest yields for the cuts with bones and boneless cuts compared to crossbreeds. Therefore, variations in karyotype are accompanied by differences in some carcass quantitative traits, i.e., 2n=36 grow and fatten slower than crossbreeds 2n=37 and 2n=38.

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