Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Mucosal Immunol ; 9(5): 1151-62, 2016 09.
Article in English | MEDLINE | ID: mdl-26732677

ABSTRACT

A characteristic feature of gastrointestinal tract inflammatory disorders, such as inflammatory bowel disease, is polymorphonuclear neutrophil (PMN) transepithelial migration (TEM) and accumulation in the gut lumen. PMN accumulation within the intestinal mucosa contributes to tissue injury. Although epithelial infiltration by large numbers of PMNs results in mucosal injury, we found that PMN interactions with luminal epithelial membrane receptors may also play a role in wound healing. Intercellular adhesion molecule-1 (ICAM-1) is a PMN ligand that is upregulated on apical surfaces of intestinal epithelial cells under inflammatory conditions. In our study, increased expression of ICAM-1 resulted in enhanced PMN binding to the apical epithelium, which was associated with reduced PMN apoptosis. Following TEM, PMN adhesion to ICAM-1 resulted in activation of Akt and ß-catenin signaling, increased epithelial-cell proliferation, and wound healing. Such responses were ICAM-1 dependent as engagement of epithelial ICAM-1 by antibody-mediated cross-linking yielded similar results. Furthermore, using an in-vivo biopsy-based, colonic-mucosal-injury model, we demonstrated epithelial ICAM-1 has an important role in activation of epithelial Akt and ß-catenin signaling and wound healing. These findings suggest that post-migrated PMNs within the intestinal lumen can regulate epithelial homeostasis, thereby identifying ICAM-1 as a potential therapeutic target for promoting mucosal wound healing.


Subject(s)
Colon/immunology , Epithelial Cells/immunology , Immunity, Mucosal , Intercellular Adhesion Molecule-1/immunology , Intestinal Mucosa/immunology , Neutrophils/immunology , Wound Healing/immunology , Animals , Biopsy , Cell Communication/immunology , Cell Line , Cell Proliferation , Colon/injuries , Epithelial Cells/pathology , Gene Expression Regulation , Humans , Intercellular Adhesion Molecule-1/genetics , Intestinal Mucosa/injuries , Male , Mice , Mice, Inbred C57BL , Neutrophils/cytology , Proto-Oncogene Proteins c-akt/genetics , Proto-Oncogene Proteins c-akt/immunology , Signal Transduction , Tissue Culture Techniques , Transendothelial and Transepithelial Migration/immunology , beta Catenin/genetics , beta Catenin/immunology
SELECTION OF CITATIONS
SEARCH DETAIL
...