ABSTRACT
We report the evaluation of the microparticle enzyme immunoassay (MEIA) for estimating vitamin B12 and the ion capture (IC) assay for estimating folate in serum. The assays were performed with the Abbott IMx analyzer. Intra-assay coefficients of variation were from 3.4% to 8.0% for vitamin B12 and from 1.5% to 4.2% for folate, respectively. Interassay coefficients of variation ranged from 6.3% to 8.8% for vitamin B12 and from 3.9% to 11.3% for folate, respectively. Linearity was satisfactory, with an analytical recovery of 119 +/- 4% for vitamin B12 and 95 +/- 8% for folate. The detection limit was 56.2 pmol/l for vitamin B12 and 1.9 nmol/l for folate. Results of these assays correlated well with those from the cloned enzyme donor immunoassay (CEDIA), our current routine method: r = 0.97 for vitamin B12 (n = 47) and r = 0.97 for folate (n = 50). The MEIA and the IC assay were easy to perform with the IMx analyzer.
Subject(s)
Folic Acid/blood , Immunoenzyme Techniques , Vitamin B 12/blood , Humans , Paraproteins/analysis , Quality Control , Reference Values , Sensitivity and SpecificityABSTRACT
We report the evaluation of the cloned enzyme donor immunoassay (CEDIA) for the estimation of phenytoin and phenobarbital in serum. The assays were performed with a Hitachi 911 analyzer. Intra-assay coefficients of variation were from 1.5 to 4.4% for phenytoin and 1.6 to 5.5% for phenobarbital. Interassay coefficients of variation ranged from 1.8 to 5.3% for phenytoin and 2.9 to 4.8% for phenobarbital. Linearity was satisfactory, with a recovery of 103% at 11 mg/L and 110% at 22 mg/L phenytoin and 93% at 14 mg/L and 101% at 40 mg/L for phenobarbital. The detection limit was 1.2 mg/L for phenytoin and 0.6 mg/L for phenobarbital. Results of this assay correlated well with those of a conventional high-performance liquid chromatography (HPLC) method; r = 0.99 for phenytoin (n = 47) and r = 0.98 for phenobarbital (n = 48). The CEDIA was easy to handle and especially suitable for short turn-around time application.
