ABSTRACT
Epithelial differentiation is an early or predisposing step in epithelial ovarian carcinogeneses which occurs in pre-neoplastic lesions, benign tumors and normal ovarian surface epithelium (OSE) of women with a familial history of ovarian cancer. During neoplastic progression, OSE acquires a more epithelial aspect, including the expression of CA125 protein and other epithelial markers, whereas mesenchymal characteristics diminish. In this study, we investigated 26 primary cell cultures, including benign and malignant OSE neoplasms, obtained from women who underwent surgical removal of the ovaries at the university hospital of the State University of Campinas (Campinas, SP, Brazil). Cell morphology was assessed from the time of cell adhesion to the substrate up to the third of fourth passage. CA125 was detected byimmunohistochemistry at each passage. Serum CA125 levels were obtained from clinical records and heredograms were constructed using the information about the recurrence of familial cancer provided by the patients. Seventy-eight percent of the malignant OSE tumors analyzed showed an epithelial cell phenotype and 71 percent were positive for CA125. Benign and normal OSE cultures had a fibroblast-like cell phenotype, a negativa CA125 expression and an inexpressive history of recurrent familial cancer, compared to malignant OSE tumors. We concluded that the expression of an epithelial phenotype in vitro may serve as an important tumor marker in malignant OSE neoplasms. In certain cases, this marker may be more reliable than the determination of serum CA125 levels. However, the relationship between the expression if the epithelial phenotype in vitro and a familial predisposition to tumors development remains to be determined.
Subject(s)
Humans , Female , Adolescent , Adult , Middle Aged , Epithelial Cells/pathology , Neoplastic Syndromes, Hereditary , Ovarian NeoplasmsABSTRACT
Restrospectiva: A fertilizaçao in vitro (FIV) e a inseminaçao artificial intra-uterina (IA) sao procedimentos que acarretam em grande estresse para o casal e supostamente a FIV seria mais estressante do que a IA. Objetivos: Estudar a influência deste estresse sobre a qualidade do sêmen. Meterial e Metodos: Comparamos a concentraçao e motilidade dos espermatozóides da última amostra de sêmen obtida durante a propedêutica de esterilidade, teoricamente sem estresse, (grupo de controle) e os mesmos parâmetros da amostra obtida na primeira tentativa de FIV ou IA, teoricamente com estresse, (grupo de estudo). Logo, comparamos os mesmos parâmetros espermáticos entre o grupo de FIV e IA. A análise estatística foi feita mediante teste t de Student. Resultados: A comparaçao entre o grupo controle e o de estudo no grupo de FIV mostrou que a média da motilidade foi significativamente maior durante o procedimento e a concentraçao de espermatozóides foi significativamente menor durante o procedimento. A comparaçao no grupo que realizou IA nao mostrou diferenças significativas na qualidade do sêmen antes e durante o procedimento. A comparaçao entre os grupos de FIV e IA nao mostrou diferenças entre eles. Conclusoes: Nossos resultados mostraram que o estresse, presumidamente provocado pelas técnicas de fertilizaçao assistida, provocaram diferenças apenas ao acaso no sêmen quando comparadas com as amostras coletadas durante a propedêutica de esterilidade.
