[Methodological approaches to creating a liposomal form of human recombinant alpha 2-interferon]. / Metodichesie podkhody k sozdaniiu liposomal'noi formy chelovecheskogo rekombinantnogo al'fa 2-interferona.

The authors have obtained several variants of liposomal forms of human alpha 2-interferon. The forms intended to preserve antiviral activity with regard to the methods of liposomal formation were comparatively studied. It has been found that liposomal formation through stirring caused no decrease in antiviral activity.

[Biotinylated pH-sensitive liposomes--containers for guided administration of biologically active substances to the cells]. / Biotinilirovannye pH-chuvstvitel'nye liposomy--konteiner dlia napravlennoi dostavki biologicheski aktivnykh veshchestv k kletkam.

The technique is described for obtaining pH-sensitive biotinylated liposomes. The liposomes included phosphatidyl ethanolamine, oleic acid, cholesterol, biotin amidocaproyl phosphatidyl ethanolamine, with a molar ratio of 7:3:3:0.1 or 4:2:4:0.1. The fluorescein stain calcein at ++self-limited concentration, 32-P-dTTP, or DNA was incorporated into the internal liposome medium. The liposomes were stable in neutral or mildly alkaline medium but became leaky and showed agglutination at pH 5.7. Liposomes were agglutinated in the presence of avidin and bound specifically to the layer of avidin on the surface of immunological plates. The apparent liposome affinity to the avidin layer was up to 10(-11) M. The avidin-biotin system allows for specific binding of liposomes to human lymphocytes via biotinylated anti-CD5-antibodies. A simple, convenient, and rapid procedure has been elaborated for the binding of water-soluble substances (plasmid DNA included) to liposomes without DNA destruction.

[The effect of high energy irradiation on the membrane potential and impulse activity of neurons in the brain of Helix pomatia]. / Vliianie vysokointensivnogo oblucheniia na membrannyi potentsial i impul'snuiu aktivnost' neironov golovnogo mozga vinogradnoi ulitki Helix pomatia.

In electrophysiological experiments with a preparation of the isolated Helix pomatia brain, a study was made of the effect of pulsed irradiation with high-energy electrons (20 MeV) on membrane potentials and pulse activity of "silent", pacemaking and postsynaptic neurons. It was shown that after irradiation with 150 and 300 Gy (dose rate 5 Gy/s and pulse frequency 50 Hz) "silent" neurons retain their excitability. Pacemaking neurons responded to radiation by a drastic increase in spontaneous pulse activity followed by its transfer to a clipped then to an irregular one. At the same time, the discharge frequency increased in the postsynaptic neurons.

[Covalent binding of phospholipids to protein globule in a system of reverse micelles]. / Kovalentnoe sviazyvanie fosfolipidov s belkovoi globuloi v sisteme obrashchennykh mitsell.

Acylation of protein amino groups by N'-hydroxysuccinimide ester of N-succinyl phosphatidylethanolamine in reverse micelles of diisooctylsulphosuccinate in hexane was studied. The experiment in a model system (glycine solution in reverse micelles) showed rate of acylation of amino groups to be by over an order of magnitude higher than rate of hydrolysis. Water-soluble proteins (alpha-chymotrypsin and IgG), modified by means of this method, can effectively bind liposomes without disturbing the integrity of liposomal membrane.