[The effect of catalytic subunit of cAMP-dependent protein kinase on the activity of cytotoxic factor from natural killer cells]. / Vliianie kataliticheskoi sub''edinitsy cAMP-zavisimoi proteinkinazy na aktivnost' tsitotoksicheskogo faktora natural'nykh killerov.

The catalytic subunit of cAMP-dependent protein kinase modifies proteins of NKCF. Several proteins of the treated NKCF can be labelled with 32P. Treatment of NKCF with protein kinase enhance the cytotoxicity of NKCF.

[Detection of a perforin-like protein from the soluble cytotoxic factor of natural killer cells]. / Obnaruzhenie perforinopodobnogo belka v sostave rastvorimogo tsitotoksicheskogo faktora natural'nykh killerov.

The protein composition of the cytolytic factor (NKCF) secreted by natural killer cells was analyzed. Natural killer cells were isolated from spleens of nude rats and purified in the Percole gradient. SDS-PAAG analysis of NKCF revealed the presence of major proteins with an approximate molecular weight of 62-67 kDa. Biochemical properties of these proteins such as fractionation on a TSK-3000 SW gel filtration column, electrophoretical mobility in reduced conditions, enhanced cytolytical activity in the presence of Ca2+ demonstrate the similarity with the pore-forming protein--perforin. Apart from the major component, proteins with m. w. 42 kDa, 31-28 kDa, 17 kDa and 12-14 kDa were also found. It is possible that the Ca2+-independent cytolytical activity is bound to one of these minor protein components of NKCF.