ABSTRACT
Agar gel precipitation test with cross-adsorbed immune sera was used for the antigenic differentiation of strains of tick-borne encephalitis virus (TBEV). Fifty strains of the Far East TBEV serotype and 46 strains of the Siberian (Aina) TBEV serotype were isolated from Ixodes persulcatus, which is the main vector of the above TBEV subtypes in the Asian and European parts of Russia. The fragment of the envelope protein gene was sequenced for TBEV strains. Sequences of new-group strains of the Siberian subtypes isolated from 3 patients with chronic TBE and from brain tissues of 4 deceased patients were determined. Lethal TBE outcomes were registered in Siberia (Irkutsk Region and Krasnoyarsk Territory) and in Russia's European part (Yaroslavl Region).
Subject(s)
Encephalitis Viruses, Tick-Borne/genetics , Encephalitis, Tick-Borne/virology , Amino Acid Sequence , Animals , Antigens, Viral/immunology , Birds/virology , Brain/virology , Chronic Disease , Encephalitis Viruses, Tick-Borne/classification , Encephalitis Viruses, Tick-Borne/isolation & purification , Encephalitis, Tick-Borne/mortality , Genes, Viral , Humans , Molecular Sequence Data , Rodentia/virology , Russia/epidemiology , Sequence Alignment , Serotyping , Ticks/virology , Viral Envelope Proteins/geneticsABSTRACT
The third outbreak of an acute infection accompanied in 3 cases by inflammation of the uveal membrane (uveitis) was recorded among infants in Krasnoyarsk in 1986. From the infants with the eye involvement as well as from those in contact with the former, 36 strains of ECHO-11 virus were isolated, and seroconversion to this virus was demonstrated. All the infants in contact with the eye patients were given gamma-globulin which seemed to limit the spread of the infection. The third outbreak of uveitis, 1986, was similar to the 1st (1980-1981) and 2nd (1982) outbreaks of uveitis in Krasnoyarsk in such aspects as the clinical eye disease (anterior uveitis-iridocyclitis), symptoms of general infection, age of the infants (3-8 months), the nosocomial pattern of infection, enterovirus etiology, marked ophthalmotropism of ECHO-11 virus isolates in monkeys.
