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1.
Genetika ; 34(1): 32-7, 1998 Jan.
Article in Russian | MEDLINE | ID: mdl-9532450

ABSTRACT

The primary structure of the cDNA copy of the evolutionary conserved gene Nc73EF from the region 73EF of Drosophila melanogaster was determined. Gene Nc73EF was shown to encode a protein highly homologous to the E1 subunit of human 2-oxoglutarate dehydrogenase, which catalyzes one of the key reactions of the Krebs cycle.


Subject(s)
Conserved Sequence , Drosophila melanogaster/genetics , Genes, Insect , Ketoglutarate Dehydrogenase Complex/genetics , Amino Acid Sequence , Animals , Base Sequence , Biological Evolution , Catalysis , Citric Acid Cycle , Genetic Code , Humans , Molecular Sequence Data , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid
2.
Bioorg Khim ; 21(9): 691-4, 1995 Sep.
Article in Russian | MEDLINE | ID: mdl-8588814

ABSTRACT

On the basis of the known primary structure of the gene for murine pancreatic ribonuclease, two deoxyoligonucleotides were selected as primers for amplification of human pancreatic ribonuclease gene. The PCR amplified DNA fragment was subsequently cloned, and its nucleotide sequence was determined. Pancreatic ribonuclease gene was localized on human chromosome 14.


Subject(s)
Chromosomes, Human, Pair 14 , Ribonuclease, Pancreatic/genetics , Amino Acid Sequence , Base Sequence , Chromosome Mapping , Cloning, Molecular , DNA , DNA Primers , Humans , Molecular Sequence Data , Polymerase Chain Reaction
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